Effects of Phosphate on Streptomyces Griseus Chitinase Production

Smacker, Richard A.; Kim, Chi K.

doi:10.1016/b978-0-12-780950-2.50030-7

Cited by 11 publications

(3 citation statements)

References 16 publications

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“…In contrast, better chitinase production was observed in the marine bacteria, Aeromonas hydrophila SBK1 when shrimp shell powder was used rather than colloidal chitin 41 . Phosphate was reported to have a negative effect on the chitinase production in Streptomyces griseus 42 , but herein, it did have a modest positive effect on chitinase production (K 2 HPO 4 ).…”

Section: Screening Of Significant Variables By Plackett-burman Designcontrasting

confidence: 52%

Bioconversion of chitin and concomitant production of chitinase and N-acetylglucosamine by novel Achromobacter xylosoxidans isolated from shrimp waste disposal area

Shanmugam

Sadaiappan

Aruni

et al. 2020

Sci Rep

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Marine pollution is a significant issue in recent decades, with the increase in industries and their waste harming the environment and ecosystems. Notably, the rise in shellfish industries contributes to tons of shellfish waste composed of up to 58% chitin. Chitin, the second most ample polymer next to cellulose, is insoluble and resistant to degradation. It requires chemical-based treatment or enzymatic hydrolysis to cleave the chitin polymers. the chemical-based treatment can lead to environmental pollution, so to solve this problem, enzymatic hydrolysis is the best option. Moreover, the resulting biopolymer by-products can be used to boost the fish immune system and also as drug delivery agents. Many marine microbial strains have chitinase producing ability. Nevertheless, we still lack an economical and highly stable chitinase enzyme for use in the industrial sector. So we isolate a novel marine bacterial strain Achromobacter xylosoxidans from the shrimp waste disposal site using chitin minimal medium. Placket-Burman and central composite design statistical models for culture condition optimisation predicted a 464.2 U/ml of chitinase production. The culture conditions were optimised for maximum chitinase production recording up to 467 U/ml. This chitinase from the A. xylosoxidans was 100% active at an optimum temperature of 45 °C (withstand up to 55 °C) and pH 8 with 80% stability. The HPLC analysis of chitinase degraded shellfish waste reveals a major amino acid profile composition-arginine, lysine, aspartic acid, alanine, threonine and low levels of isoleucine and

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Section: Screening Of Significant Variables By Plackett-burman Designcontrasting

confidence: 52%

Bioconversion of chitin and concomitant production of chitinase and N-acetylglucosamine by novel Achromobacter xylosoxidans isolated from shrimp waste disposal area

Shanmugam

Sadaiappan

Aruni

et al. 2020

Sci Rep

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“…Excess concentrations of phosphate will suppress the growth of some strains such as Streptomyces viridochromogenes [28]. Increased phosphate was reported to be inhibitory to chitinase production and morphogenesis in Streptomyces [15,29]. The above results indicated that the Plackett-Burman design is power tool for identifying factors, which have significant influence on chitinase activity.…”

Section: Screening Of Important Medium Componentsmentioning

confidence: 92%

Statistical optimization of medium components to improve the chitinase activity of Streptomyces sp. Da11 associated with the South China Sea sponge Craniella australiensis

Han

Miao

et al. 2008

Process Biochemistry

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“…[37,38] Chitinase production from Streptomyces was inhibited by increased phosphate. [39] The above results showed that the PlackettÀBurman experimental design is a powerful tool for screening factors, which have significant effects on chitinase activity. [40] Optimization of medium components by CCD CCD was used to determine the optimal level of the three selected variables (crab shell powder, CaCl 2 ¢2H 2 O and yeast extract) for the chitinase production.…”

Section: Selection Of Important Medium Components Using Plackettàburmmentioning

confidence: 99%

Statistical optimization of medium components for chitinase production byPseudomonas fluorescensstrain HN1205: role of chitinase on egg hatching inhibition of root-knot nematode

Lee

Kim

2015

Biotechnology & Biotechnological Equipment

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In the present research, statistical PlackettÀBurman design (PBD) and central composite design (CCD) were used to optimize the medium's components in order to enhance the chitinase activity of Pseudomonas fluorescens strain HN1205. Among the seven nutritional elements that were studied, crab shell powder, CaCl 2 ¢2H 2 O and yeast extract were proved using PBD to have significant effect on chitinase activity. An optimal medium was obtained by applying a three-factor central composite design, which consisted of crab shell powder (1.0 g/L), CaCl 2 (0.5 g/L) and yeast extract (0.5 g/L), with the highest chitinase activity of 1.03 U/mL. This value was 2.87-fold higher than the activity obtained in the lowest productive medium in the design matrix. The chitinase produced by the HN1205 strain was partially purified with 80% ammonium sulphate and anion-exchange chromatography and assessed for inhibition of hatching of nematode eggs. The partially purified chitinase significantly reduced the hatch of Meloidogyne incognita eggs (8.1%), as compared to the effect of 10 mmol/L Tris-HCl buffer (pH 8.0) (49.4%) and boiled chitinase (54.7%). This study demonstrated the role of chitinolytic enzymes produced by P. fluorescens strain HN1205. The obtained optimal activity proved that the enzymes could be potential biological control agents.

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Effects of Phosphate on Streptomyces Griseus Chitinase Production

Cited by 11 publications

References 16 publications

Bioconversion of chitin and concomitant production of chitinase and N-acetylglucosamine by novel Achromobacter xylosoxidans isolated from shrimp waste disposal area

Bioconversion of chitin and concomitant production of chitinase and N-acetylglucosamine by novel Achromobacter xylosoxidans isolated from shrimp waste disposal area

Statistical optimization of medium components to improve the chitinase activity of Streptomyces sp. Da11 associated with the South China Sea sponge Craniella australiensis

Statistical optimization of medium components for chitinase production byPseudomonas fluorescensstrain HN1205: role of chitinase on egg hatching inhibition of root-knot nematode

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