Effects of phosphatidylserine‐containing supported lipid bilayers on the polarization of macrophages

Abstract

Placement of dental implants initiates inflammatory foreign body response, in which macrophages play a central role and affect the subsequent tissue healing process such as bone formation. The purpose of this study was to fabricate phosphatidylserine (PS)-containing supported lipid bilayers (SLBs) on a titanium surface to regulate the polarization of macrophages, a critical factor that affects following tissue healing and regeneration. The fluorescent recovery after photobleaching images showed that the percentage of PS had a critical influence on the fluidity, and 20% PS had the highest fluidity. Furthermore, more expanded and elongated cells were observed in the SLB-coated groups. transforming growth factor-β1 and vascular endothelial growth factor, the key cytokine markers of M2 macrophage polarization, were increased in the SLB-coated groups, especially in the 20% PS group. Consistently, cells cultured on the SLB-coated titanium exhibited the distribution of CD206 , which is a M2 macrophage specific maker. The results of this study demonstrated M2 polarization of macrophages on PS-SLB-coated titanium discs, which suggests the application of PS-SLB as an immune-regulating coating material to improve tissue reactions to dental implants. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 2625-2633, 2018.

Nanotubular TiO₂ regulates macrophage M2 polarization and increases macrophage secretion of VEGF to accelerate endothelialization via the ERK1/2 and PI3K/AKT pathways

“…40 Furthermore, other studies have confirmed that elongated macrophages on the surface of modified biomaterials exhibit the M2 phenotype. [41][42][43] However, some other studies have demonstrated opposing results. 44 Therefore, future research must investigate the detailed mechanisms through which TiO 2 nanotube surfaces regulate macrophage polarization.…”

Dopamine-Mediated Zwitterionic Polyelectrolyte-Coated Polypropylene Hernia Mesh with Synergistic Anti-inflammation Effects

“…When macrophages are activated into proinflammatory phenotype, that is M1, the cell morphology tends to be polygonal shaped with extended pseudopods. ,, As shown in Figure c, macrophages adhered to rPP transformed into irregular or polygonal shape with clear and long extended pseudopods, indicating the typical morphology of M1. , On PDA-PP, macrophages also spread but did not have the same long extended pseudopods as on rPP, which suggests decreased adhesion and moderate activation of macrophages. In contrast, macrophages adhered to coated meshes extended poorly and had a round-like shape.…”

Modulation of Macrophage Polarization by Phospholipids on the Surface of Titanium

“…A PS-containing supported phospholipid bilayer was bound to induce the M2 phenotype of RAW 264.7 cells on a Ti surface [20]. It was theorized that PS polarizes the macrophage-like cells by binding to the PS receptors, mimicking the interaction between apoptotic cells and macrophages.…”

“…Therefore, Ti surfaces coated with PS are also expected to induce M2 macrophage polarization and influence osseointegration. In fact, a supported lipid bilayer containing PS induced M2-like polarization of the murine macrophage cell line, RAW 264.7, on Ti surfaces [20]. Besides the formation of the lipid bilayer, Ti has been coated with PS-containing phospholipids through the evaporation of an organic solvent and the deposition of phospholipids on the Ti surface [18,21].…”