Effects of Photodynamic Therapy Using Yellow LED-light with Concomitant Hypocrellin B on Apoptotic Signaling in Keloid Fibroblasts

Hu, Yongqing; Zhang, Chunmin; Li, Shengli; Yang, Jiao; Qi, Tonggang; Guo, Wei; Han, Gangwen

doi:10.7150/ijbs.17920

Cited by 23 publications

(12 citation statements)

References 32 publications

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“…Some of the preceding studies suggest that PDT induces apoptotic cell death. 20,22–25 By contrast, the study by Liu et al 21 suggests that PDT may induce autophagic cell death that is dependent on superoxide anions. Thus, when cultured fibroblasts isolated from the excised keloids of six patients were treated with ALA-PDT, the fibroblasts died.…”

Section: Resultsmentioning

confidence: 99%

“…Several in vitro studies have also examined the effect on keloid fibroblasts of PDT employing new photosensitisers. Hu et al 24 found that PDT with the natural perylenequinone photosensitiser hypocrellin B and yellow light significantly reduced keloid fibroblast viability by inducing apoptosis (as shown by upregulated BAX expression and caspase-3 activation and downregulated BCL-2 expression). Moreover, Zheng et al 25 explored the role of RUNX3 in the response of keloid fibroblasts to pheophorbide a-based PDT (Pa-PDT).…”

Section: Resultsmentioning

confidence: 99%

“…38 The cell death may be via apoptosis, necrosis and/or autophagy. 24 These changes may alter growth factor and cytokine expression in the lesion, thereby modulating collagen production and extracellular matrix organisation. This is supported by the fact that ALA-PDT on cultured normal fibroblasts significantly reduces their type I collagen synthesis and upregulates their collagenolytic enzymes.…”

Section: Discussionmentioning

confidence: 99%

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Photodynamic therapy for keloids and hypertrophic scars: a review

Tosa

Ogawa

2020

Scars, Burns & Healing

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Purpose: Keloid is a poorly understood disease that is unique to humans. Hypertrophic scars are similar to keloids and may transform into keloids over time. The standard treatments for these scars are limited by inconsistent efficacy and long treatment/follow-up times. Therefore, a new treatment that is effective for all abnormal scar cases is needed. One option may be photodynamic therapy (PDT). This review assesses the current evidence regarding the safety and efficacy of PDT for keloids and hypertrophic scars. Methods: PubMed, Medline and Web of Science were searched from 1900 onwards for the following terms: ‘keloid and photodynamic therapy (PDT)’; ‘hypertrophic scar and photodynamic therapy (PDT)’; and ‘scar and photodynamic therapy (PDT)’. Articles were included if they reported using topical PDT to treat keloids or hypertrophic scars, the patient(s) had one or more keloids and/or hypertrophic scars, and the effect of PDT on these abnormal scars was described. Results: In total, 538 articles were identified. Thirteen fulfilled all inclusion criteria. Eight were laboratory studies on keloid/hypertrophic scar explants, fibroblasts or tissue-engineered skin models and five were clinical studies/case reports. The clinical results of PDT on keloids and hypertrophic scars are encouraging. Conclusion: PDT appears to play a promising role in keloid and hypertrophic scar therapy but additional clinical studies, particularly randomised clinical trials, are needed.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Photodynamic therapy for keloids and hypertrophic scars: a review

Tosa

Ogawa

2020

Scars, Burns & Healing

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“…Secondary antibodies were conjugated to either FITC or Dylight 549 for 1 h and the nucleus was counterstained with DAPI. Furthermore, Bcl‐2 and Bax protein expression was examined using flow cytometry intracellular staining as previously described …”

Section: Methodsmentioning

confidence: 99%

Detection and Evaluation of Anti‐Cancer Efficiency of Astragalus Polysaccharide Via a Tissue Engineered Tumor Model

Wang

et al. 2018

Macromolecular Bioscience

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Polysaccharides have been known to display their anti-cancer activity via immunomodulation. The immunomodulation of RAW 246.7 macrophages by astragalus polysaccharide (APS) is herein studied in human breast cancer cells. Apart from traditional 2D culture, a novel tissue-engineered tumor model is prepared based on decellularized porcine lung scaffold. Decellularized lung scaffolds exhibit preferable biocompatibility that promote the formation and enlargement of tumor spheroids. The conditioned medium (CM, the supernatant liquid of APS-treated RAW264.7 macrophages) shows anti-cancer activity by inhibiting cell proliferation, demonstrated by a 39.25 ± 5.04% decrease in tumoroid size and 20.96 ± 2.43% reduction in cell viability, and promoting cell apoptosis by the nuclear fragmentation and increasing apoptotic-stage proportion. The cytotoxicity of CM is associated with the upregulated production of nitric oxide and tumor necrosis factor-α from RAW 246.7 cells stimulated by APS. Overall, by using this 3D tumor model to study CM, there is convincing evidence that APS can modulate macrophage function to further mediate anti-cancer activity.

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“…In contrast to many other PDT for deep-seated tumors, PDT for skin lesions do not restrict to limited well-penetrated near-infrared light. Studies reported that yellow LED-light, blue of argon lasers and other invisible light sources were chosen for superficial lesions, not confined to abnormal scar in PDT, and resulting in good therapeutic effects ( Hu et al, 2017 ; Tosa and Ogawa, 2020 ). Furthermore, because of less painful, more convenient, daylight photodynamic therapy (dPDT) by using sunlight as a light source became an effective alternative therapy for many superficial skin lesions ( Lee C. N et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Visible light-driven photodynamic therapy for hypertrophic scars with MOF armored microneedles patch

et al. 2023

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Photodynamic therapy (PDT) is widely used for the treatment of hypertrophic scars in clinical practice. However, the low transdermal delivery of photosensitizers in scar tissue and protective autophagy induced by Photodynamic therapy greatly reduces the therapeutic efficiency. Therefore, it is necessary to deal with these difficulties for overcoming obstacles in Photodynamic therapy treatment. In this study, a photosensitizer with photocatalytic performance was designed and synthesized using innovative MOFs (metal-organic frameworks). Additionally, the MOFs, together with an autophagy inhibitor chloroquine (CQ), was loaded in a high mechanical strength microneedle patch (MNP) for transdermal delivery. With these functionalized MNP, photosensitizers and chloroquine were delivered deep inside hypertrophic scars. Inhibition of autophagy increases the levels of reactive oxygen species (ROS) under high-intensity visible-light irradiation. Multiprong approaches have been used to remove obstacles in Photodynamic therapy and successfully enhance its anti-scarring effect. In vitro experiments indicated that the combined treatment increased the toxicity of hypertrophic scar fibroblasts (HSFs), downregulated the level of collagen type I expression as well as transforming growth factor-β1 (TGF-β1)expression, decreased the autophagy marker protein LC3II/I ratio, increased the expression of P62. In vivo experiments showed that the MNP had good puncture performance, and significant therapeutic effects were observed in the rabbit ear scar model. These results indicate that functionalized MNP has high potential clinical value.

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Effects of Photodynamic Therapy Using Yellow LED-light with Concomitant Hypocrellin B on Apoptotic Signaling in Keloid Fibroblasts

Cited by 23 publications

References 32 publications

Photodynamic therapy for keloids and hypertrophic scars: a review

Photodynamic therapy for keloids and hypertrophic scars: a review

Detection and Evaluation of Anti‐Cancer Efficiency of Astragalus Polysaccharide Via a Tissue Engineered Tumor Model

Visible light-driven photodynamic therapy for hypertrophic scars with MOF armored microneedles patch

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