Effects of phthalates on bovine primary testicular culture and spermatozoa

Dikmen, Begüm Yurdakök; Stelletta, Calogero; Tekin, Koray; Kuzukiran, Ozgur; Daşkın, Ali; Filazı, Ayhan

doi:10.1007/s10616-019-00336-z

Cited by 11 publications

(8 citation statements)

References 47 publications

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“…According to our previous study in bulls (Yurdakok-Dikmen et al 2019), the toxicity of the HMWP in average for the tested primary cultures (IC 50 average 1.79 ng/mL) was higher than the LMWP (IC 50 average 3.64 ng/mL); similar to the spermiotoxicity results (1.71 and 2.12 ng/mL, respectively). In accordance with the previous research in the bulls, our results were contradicted with the general hypothesis that the LMWP have adverse effects at lower concentrations (The Danish Environmental Protection Agency 2013).…”

Section: Discussionsupporting

confidence: 80%

“…Furthermore, the combined effects of phthalates can alter the semen quality more than the effect of a single substance, depending on the concentration ratio (Lea et al 2016). Compared to our previous study (Yurdakok-Dikmen et al 2019) regarding the effects of phthalates on bovine testicular parenchymal cells; dog cells were found less susceptible. Therefore, even though, various researchers agree on the opinion that testicular cell cultures are valuable tool regarding the clarification of the mechanism in testicular damage and screening compounds (Gray and Beamand 1984); these species-specific differences should be considered as a potential tool for comparative male reproductive toxicity.…”

Section: Discussioncontrasting

confidence: 71%

“…In the present study, DMP was determined as the least toxic phthalate with an IC 50 value of 169.1 nM, whereas DEHP has shown the most toxic effects, with an IC 50 value of 22.5 nM, on the primary cells of dog testicular parenchyma. In our previous study (Yurdakok-Dikmen et al 2019), the effects of seven phthalates (BBP, DBP, DIBP, DINP, DEHP, DEP and DMP) were tested on bovine primary testicular and epididymal cells in vitro. According to the obtained results, IC 50 values were ranged from 0.15 to 4.11 ng/ mL for the parenchymal cells whereas a range of 0.01-7.31 ng/mL was observed for mediastinum wherein the cytotoxic effects were more evident compared to parenchymal section.…”

Section: Discussionmentioning

confidence: 99%

“…The primary testis parenchymal cell cultures were made according to Yurdakok-Dikmen et al 2017 and under the laminar flow Class 2 (BioSafety Cabinet Telstar Bio II Advance 4, Terrassa, Spain). The cell cultures chemicals were obtained from Sigma Chemical.…”

Section: Primary Dog Testis Parenchymal Cell Culturementioning

confidence: 99%

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Companion animals get close to the toxic aspects of antropogenic world: cytotoxicity of phthalates and bisphenol A on dog testicular primary cells

et al. 2020

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Phthalates, which are among the most abundant plasticizers, have detrimental effects on the reproductive system. Similar to human, dogs are prominently exposed to phthalates in daily routines at low concentrations; while toys, training devices and commercial dog foods are considered as the primary sources of exposure. This study aimed to reveal and compare the cytotoxic effects of selected phthalates (Benzyl butyl phthalate (BBP), Diethyl phthalate (DEP), Bis (2-ethylhexyl) phthalate (DEHP), Di-'isobutyl' phthalate (DIBP), Di-'isodecyl' phthalate (-DIDP) Di-'isononyl' phthalate (DINP), Dimethyl phthalate (DMP), Di-n-octyl phthalate (DNOP)), and Bisphenol A (BPA) following 24 h exposure on primary testicular parenchymal cells of dog in vitro at concentrations between 0.001 and 2.5 nM. According to cytotoxicity results, DEHP was found to be the most toxic phthalate with IC 50 at 22.53 lM; while DMP was the least (169.17 nM). IC 50 of BPA was 161.81 nM, less than the average (61.95 nM) of phthalates. In addition, dog primary testicular cells were found more susceptible to the high molecular weight phthalates (DNOP, DEHP, DINP, DIDP) than low molecular weight phthalates (DMP, DEP, DIBP, BBP). Further studies should focus on morphological, physiological and molecular differences to comprehend the mechanisms involved as well as decreasing the risk for impaired spermatogenesis caused by environmental toxicants in companion animal medicine.

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Section: Discussionsupporting

confidence: 80%

Section: Discussioncontrasting

confidence: 71%

Section: Discussionmentioning

confidence: 99%

Section: Primary Dog Testis Parenchymal Cell Culturementioning

confidence: 99%

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Companion animals get close to the toxic aspects of antropogenic world: cytotoxicity of phthalates and bisphenol A on dog testicular primary cells

et al. 2020

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“…However, according to current scientific data, the toxic effects of TEB on the reproductive system of male livestock are still unclear. Primary testicular cultures and spermiotoxicity assays offer valid and valuable data in in vitro studies in which the species-specific effects of environmental pollutants on the reproductive system were screened (Yurdakok-Dikmen et al, 2019). Thus, the objective of the present study was to evaluate the effects of TEB on the male reproductive system in terms of physiological functions and cytotoxicity by the use of bovine testicular cell cultures and epididymal spermatozoa for evaluation.…”

Section: Introductionmentioning

confidence: 99%

Assessment of tebuconazole exposure on bovine testicular cells and epididymal spermatozoa

Kabakçi

Kaya

Yiğit

et al. 2021

AVet

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This study is the first to investigate the effects of tebuconazole (TEB) on the physiological functions of bovine testicular cells and epididymal spermatozoa. Motility and plasma membrane integrity of spermatozoa exposed to TEB (0.001–100 µM) were evaluated at different incubation times (0–6 h), while TEB-induced spermiotoxicity was assessed after 24 h in cell cultures. Testicular cells, obtained from the parenchyma of bovine testes, were seeded at 1.0 × 104 and 1.5 × 106 cells/well in 96- and 12-well culture plates and incubated for 48 h in culture media containing TEB (0.001–100 µM) to evaluate cytotoxicity and hormone release, respectively. TEB did not affect the motility and plasma membrane integrity. However, significant spermiotoxicity occurred at higher TEB (1–100 µM) concentrations (P < 0.05) compared to control and lower doses. Although no dose caused cytotoxicity in testicular cells (P > 0.05), 1 and 100 µM TEB caused a significant increase in testosterone secretion (P < 0.05). As a result, high doses of TEB (1–100 µM) had slightly suppressive effects on spermatozoa; however, these doses had stimulatory effects on testosterone secretion by testicular cells. It appears that the disruption of hormonal homeostasis of testicular cells after TEB exposure may result in metabolic and especially reproductive adverse effects in bulls.

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One Health challenges for pig reproduction

Peltoniemi

Tanskanen

Kareskoski

2023

Molecular Reproduction Devel

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The current state of the world challenges pig reproduction as an important part of One Health, which involves interrelationships between animal, human and environmental health. The One Health concept underlines a comparative aspect in reproductive physiology and disease occurrence, bridging knowledge from one species to another. Seasonal changes in the environment affect pig reproduction and climate change may further strengthen those effects. Endocrine‐disrupting chemicals (EDCs), and specifically phthalates and heavy metals, interfere with endocrine function, and thereby sexual behavior, fertilization capacity and steroidogenesis. Reproductive infections and extended semen storage are important indications for antimicrobial use. Innovative solutions are needed to explore alternatives to antimicrobials. Efforts to ensure reproductive efficiency have prolonged farrowing as litter size has doubled over the past three decades, compromising immune transfer and welfare. Physiological, metabolic and programming related events around parturition are key areas for future One Health research in pig reproduction. In conclusion, climate change challenges reproductive management and breeding. More resilient pigs that can tolerate harsh environment but maintain high reproductive performance are needed. EDCs continue to grow as an environmental challenge for reproductive management and alternatives to antibiotics will be required.

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Effects of phthalates on bovine primary testicular culture and spermatozoa

Cited by 11 publications

References 47 publications

Companion animals get close to the toxic aspects of antropogenic world: cytotoxicity of phthalates and bisphenol A on dog testicular primary cells

Companion animals get close to the toxic aspects of antropogenic world: cytotoxicity of phthalates and bisphenol A on dog testicular primary cells

Assessment of tebuconazole exposure on bovine testicular cells and epididymal spermatozoa

One Health challenges for pig reproduction

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