Effects of Phytohæmagglutinin on Blood-Cultures of Chronic Lymphocytic Leukæmias

The Potential of Lymphocytes from Patients with Leukaemia and Reticuloses to Transform under the Influence of Phytohaemagglutinin

“…In our series we have noticed that five of the 'good responders' had total lymphocyte counts of less than 18 000, whereas all of the 'poor responders' had high total lymphocyte counts. Such a cor relation with lymphocyte content of the peripheral blood has been noted previous (3,16). One possible explanation for these findings may lie in the recent studies of lymphocyte life-span.…”

“…In an earlier report we described such a normal degree of transformation (7), and more recently we have also described a low degree of transformation in a group of patients with this disease who had the complication of hypogammaglobulinaemia (8). Others have regularly found a decreased degree of transformation in cultures of lymphocytes from patients with this type of leukaemia (1,2,3,16,19,21). In this paper we de scribe the transformation of lymphocytes from a group of patients with chronic lymphatic leukaemia, and also the effect of normal plasma on these cells, and of leukaemic plasma on normal cells.…”

Quantitative Studies of Macrophages in Blood Cultures in Chronic Lymphocytic Leukaemia

“…On the other hand, macrophages are numerous in normal blood cultures without PHA [1, 2, 14], It has been suggested that, in addition to blood monocytes, lymphocytes not engaged in blastoid transformation could be the source of macrophages in culture [2,6,8,15], It is well known that in chronic lymphocytic leukaemia (CLL) the PHA blastic transformation of blood lymphocytes in vitro is low [3,4,7,10,11,13], the majority of lymphocytes being unresponsive to PHA. In the present paper we investigated whether a relationship exists be tween the extent of macrophage appearance in blood cultures without PHA and the percentage of blastic transformation in PHA cultures of lymphocytes from normal subjects and from patients with CLL.…”

Zur Darstellung der Transformationsfähigkeit von Lymphozytenkulturen bei verschiedenen Erkrankungen mittels Feulgen – Zytophotometrie

“…zwischen dem Krankheitsbeginn [6] und der Transformationsfähigkeit an. Bernard [7] beobachtete die tiefste bei hohen Leukozytenwerten und vermutete zunächst, dass neben den pathologischen Zellen noch normale Lymphozyten vorliegen könnten, auf die die Umwandlungsfahigkeit beschränkt sei. Indessen sind bei der CLL auch transformierte Zellen mit abnormalen Chromosomenverhältnissen gefunden worden [17].…”