The Potential of Lymphocytes from Patients with Leukaemia and Reticuloses to Transform under the Influence of Phytohaemagglutinin

PHA Response of Blood and Lymph Node Lymphocytes <i>in vitro</i> in Malignant Lymphomas

“…In blood cultures from cases of Hodgkin's disease, the percentage of PHA-transformed cells has been reported to be either decreased [16,17,21], normal [8,26], or varying from case to case [6,13,31]. In cases of lymphosarcoma or reticulum cell sarcoma, the PHA response of blood lymphocytes has been found to be as high as in normal subjects [31,33] or decreased [5,15,23].…”

“…In cases of lymphosarcoma or reticulum cell sarcoma, the PHA response of blood lymphocytes has been found to be as high as in normal subjects [31,33] or decreased [5,15,23]. On the other hand, in CLL the PHA response of peripheral blood lymphocytes has constantly been reported to be very low [4,5,8,14,23,27,31,33], Therefore, it seems that, with the exception of CLL, the peripheral blood cytology does not reflect exactly the pathological status of lymphoid cells at the sites primarily involved in lymphoproliférative disorders.…”

Quantitative Studies of Macrophages in Blood Cultures in Chronic Lymphocytic Leukaemia

“…On the other hand, macrophages are numerous in normal blood cultures without PHA [1, 2, 14], It has been suggested that, in addition to blood monocytes, lymphocytes not engaged in blastoid transformation could be the source of macrophages in culture [2,6,8,15], It is well known that in chronic lymphocytic leukaemia (CLL) the PHA blastic transformation of blood lymphocytes in vitro is low [3,4,7,10,11,13], the majority of lymphocytes being unresponsive to PHA. In the present paper we investigated whether a relationship exists be tween the extent of macrophage appearance in blood cultures without PHA and the percentage of blastic transformation in PHA cultures of lymphocytes from normal subjects and from patients with CLL.…”

Zur Darstellung der Transformationsfähigkeit von Lymphozytenkulturen bei verschiedenen Erkrankungen mittels Feulgen – Zytophotometrie

“…In der PHA-Kultur änderte sich in unseren Versuchen die Verteilung der DNS-Werte und der mittleren Kerndiameter kaum. Über gleiche Ergebnisse einer stark herabgesetzten Transformation wird von den meisten Untersuchern berichtet [12,13,22,26,34,36,37,38,39,44,46], sowohl nach unspezifischcr als auch spezifischer Stimulierung, wobei meist keine Mitosen auf traten.…”