Effects of Pigment-Protein Fraction from Nannocloropsis Oculata on TNFα and IL-6 which Act as an Anti-Inflammatory Against Viral Nervous Necrosis (VNN) Infection

Yanuhar, Uun

doi:10.1016/j.proche.2015.03.059

Cited by 10 publications

(10 citation statements)

References 4 publications

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“…As a proinflammatory mediators, Hsp can release both the necrotic and non-necrotic substance into the extracellular environment, and produce a variety of immune and inflammatory responses, including activation of immune system effector and cytokine release. Hsp70 in the brains of experimental animals infected antigen found decreased levels of TNF- and IL6 [13][14].…”

Section: Discussionmentioning

confidence: 98%

Heat Shock Protein (Hsp) Response Within RNA Viral Nervous Necrosis (VNN) that Infect of the Humpback grouper Cromileptes altivelis

Yanuhar¹,

Christiawan²,

Mahmudi³

et al. 2015

3rd International Conference on Chemical, Agricultural and Medical Sciences (CAMS-2015) Dec. 10-11, 2015 Singapore

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Abstract-Induction of Hsp can trigger by cellular stressorscaused by viral infections. The aim of this study is to find out of the expression of Hsp70 on C.altivelis exposured by infection of VNN. The methods were to isolate fractionate the protein of C.altivelis organs both normal and infected by of VNN by SDS-Page, and to detect the expression of Hsp70 qualitatively on tissue using Immunohistochemistry (IHC) methods. The result shows the IHC test was a positive Hsp70. The infection of VNN that have immunogenic protein on tissue organ of C.altivelis had been reacted by expression of function Hsp70. It means antigen determinant from the immunogenic protein of VNN has sensitivity highly on the reaction of Hsp70. Hsp70 can induce immune cell for the maintenance of Humpback grouper tissue receptors. The conclusion was the infection of VNN of C.altivelis was related to the expression of Hsp70.

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Section: Discussionmentioning

confidence: 98%

Heat Shock Protein (Hsp) Response Within RNA Viral Nervous Necrosis (VNN) that Infect of the Humpback grouper Cromileptes altivelis

Yanuhar¹,

Christiawan²,

Mahmudi³

et al. 2015

3rd International Conference on Chemical, Agricultural and Medical Sciences (CAMS-2015) Dec. 10-11, 2015 Singapore

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“…PPF application on humpback grouper specifically regulate TNF and IL-6 expression indicating an increase in fish immune. The expression of antivirus genes and cytosine is mainly due to β-actin effect (Yanuhar, 2015). β-actin has a vital role in remodeling chromatin group, RNA polymerase complex, and some ribonucleic protein (McDonald et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

“…oculata used in this study was acquired from BBAP Situbondo, filtered until 150 g wet paste was obtained. The isolation method referred to Yanuhar (2015), where the cells were homogenized in a mortar for one hour with liquid nitrogen addition. 8 mL from 50 mM glycine and 20 mM KCl (pH 7.5) were added then centrifuged at 12,000 rpm for 60 min at 4 °C temperature.…”

Section: Nannochloropsis Oculatamentioning

confidence: 99%

The application of pigment-protein fraction from Nannochloropsis oculata on β-actin response of Cromileptes altivelis infected with viral nervous necrosis

Yanuhar¹,

Khumaidi²

2017

Jurnal Akuakultur Indonesia

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ABSTRACTβ-actin is a prominent protein in the immune system. An outcome from gene transcription, the protein protects against pathogen infection, such as receptor clustering, antigen internalization, and regulating vesicle for antigen processing. ß -actin expression will determine the success of immune response of the organism. This study aimed to understand the role of pigment-protein fraction (PPF) from Nannochloropsis oculata in the increase of β-actin expression in humpback grouper Cromileptes altivelis infected by viral nervous necrosis (VNN). The experiment was performed with one negative control (A: normal fish) and three PPF treatments (B: fish + PPF, C: fish + VNN, and D: fish + PPF + VNN). PPF was applied through the sonde method and analyzed with immunohistochemistry technique and Immunoratio software. The results showed that PPF was able to increase β-actin expression on all treatments: A (34.9%), B (38.1%), C (39.1%), and D (51.6%). It demonstrated PPF ability to induce the increase of β-actin expression indicating an improved defense of humpback grouper C. altivelis against VNN infection.Keywords: β-actin, pigment-protein fraction, Nannochloropsis oculata, VNN ABSTRAK β-aktin adalah protein yang berfungsi penting sebagai salah satu sistem pertahanan tubuh. Protein ini merupakan hasil transkripsi dari gen yang memiliki fungsi penting dalam sistem pertahanan tubuh terhadap infeksi patogen, seperti kelompok reseptor, internalisasi antigen, dan mengatur keluar masuknya vesikel untuk pemrosesan antigen. Ekpresi β-aktin menjadi penentu berhasil atau tidaknya respons imun pada tubuh organisme. Penelitian ini bertujuan untuk mengetahui peran fraksi protein pigment (FPP) dari Nannochloropsis oculata dalam meningkatkan respons β-aktin pada ikan kerapu tikus Cromileptes altivelis yang terinfeksi viral nervous necrosis (VNN). Pengamatan dilakukan pada empat percobaan yaitu, satu kontrol (A : ikan normal) dan tiga perlakuan FPP (B : ikan + FPP, C: ikan + VNN, dan D: ikan + VNN + FPP). Pemberian FPP menggunakan metode stoned dan analisis hasil penelitian menggunakan teknik imunohistokimia dan Immunoratio. Hasil penelitian ini menunjukkan bahwa pengiduksian FPP mampu meningkatkan ekpresi β-aktin yaitu A (34,9%), B (38,1%), C (39,1%), dan D (51,6%). Hal tersebut menunjukan FPP mampu menjadi inducer yang baik untuk peningkatan ekpresi β-aktin. Meningkatnya ekspresi β-aktin bisa dijadikan sebagai indikator pertahanan tubuh ikan kerapu tikus C. altivelis terhadap infeksi VNN.Kata kunci: β-aktin, fraksi protein pigmen, Nannochloropsis oculata, VNN

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“…As a member of heat shock protein (HSP) family, HSP 70 plays an important role in the immune response by functioning as a chaperone in immune system [15]. Both interferon g (IFN g) and interleukin 6 (IL 6) are involved in immune and inflammatory responses in fishes [16]. In fishes, the insulin-like growth factor (IGF) system plays a crucial role in stimulating cell growth, differentiation, metabolism and reproduction, while insulin-like growth factor binding proteins (IGFBPs) are present in various biological fluids, regulating the availability of IGFs to the IGF receptors [17].…”

Section: Rna Extraction and Quantitation Of Gene Expressionsmentioning

confidence: 99%

Metabolite and gene expression responses in juvenile flounder Paralichthys olivaceus exposed to reduced salinities

Liu

et al. 2017

Fish & Shellfish Immunology

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a b s t r a c tSeawater salinity is one of the most important changeable environmental factors influencing the behavior, survival, growth and production of marine organisms. In this work, metabolite and gene expression profiles were used to elucidate the biological effects of reduced salinities in juvenile flounder Paralichthys olivaceus. Metabolic profiling indicated that both reduced salinities (23.3‰ and 15.6‰) enhanced proteolysis and disturbed osmotic regulation and energy metabolism in juvenile flounder P. olivaceus. Furthermore, the low salinity (15.6‰) enhanced anaerobic metabolism indicated by the elevated lactate in flounder tissue extracts. Gene expression profiles exhibited that reduced salinities could induce immune stress and oxidative stress and disturb energy metabolism in juvenile flounder P. olivaceus. In addition, reduced salinities might promote the growth and gonadal differentiation in juvenile flounder P. olivaceus.

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Effects of Pigment-Protein Fraction from Nannocloropsis Oculata on TNFα and IL-6 which Act as an Anti-Inflammatory Against Viral Nervous Necrosis (VNN) Infection

Cited by 10 publications

References 4 publications

Heat Shock Protein (Hsp) Response Within RNA Viral Nervous Necrosis (VNN) that Infect of the Humpback grouper Cromileptes altivelis

Heat Shock Protein (Hsp) Response Within RNA Viral Nervous Necrosis (VNN) that Infect of the Humpback grouper Cromileptes altivelis

The application of pigment-protein fraction from Nannochloropsis oculata on β-actin response of Cromileptes altivelis infected with viral nervous necrosis

Metabolite and gene expression responses in juvenile flounder Paralichthys olivaceus exposed to reduced salinities

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