Effects of Plant Growth Regulators and Saccharide on In Vitro Plant and Tuberous Root Regeneration of Cassava (Manihot esculenta Crantz)

Fan, Mingxia; Liu, Zaochang; Zhou, Liguo; Lin, Tong; Liu, Yunhua; Luo, Lijun

doi:10.1007/s00344-010-9163-y

Cited by 24 publications

(20 citation statements)

References 25 publications

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“…The use of NAA (1 to 1.5 mg/L) relatively produced higher number of roots as compared to BAP (0.05 to 0.1 mg/L). These results confirm the report of Fan et al (2011) that NAA (0 to 2.0 mg/L) proved to be effective on root development in cassava. Also, Kane (2005) similarly reported that NAA (0.01 to 10 mg/L) is the most widely used and effective plant growth regulators for root induction.…”

Section: Effect Of Hormones On In Vitro Root Number Per Plantlet Of Tsupporting

confidence: 92%

Development of in vitro propagation protocol for some recalcitrant cassava (Manihot esculenta Crantz) genotypes in Sierra Leone

Sesay¹,

Yamba²,

Sherman-Kamara³

et al. 2018

Afr. J. Biotechnol.

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A study on rapid propagation of cassava through tissue culture was conducted with three elite cassava genotypes: Slicass 6, Slicass 11 and Cocoa from Sierra-Leone. They showed slow growth in Murashige & Skoog (MS) basal medium which was proven to be optimal for a vast number of cassava accessions. Prior to mutation induction, a large population needs to be produced for mutagen susceptibility test and for mutant population development. The ultimate objective of this study was to investigate the effects of plant growth regulators on the shoot development of three cassava genotypes. In vivo shoot tips were sterilized and sub-cultured on MS media supplemented with six combinations of plant growth regulators (PGRs) at different concentrations. The results showed that from all media used, the MS medium with 1.0 mg/L α-naphthalene acetic acid (NAA) showed the best response for rooting (5.50), fresh weight (0.29 g), root number (10.00) and plantlet height (3.81 cm), while 0.1 mg/L 6-benzylaminopurine (BAP) was found to be more favourable to shoot development of leaves (6.38). The highest plant height and fresh weight were 3.81 cm and 0.29 g, respectively for Cocoa at 1.0 mg/L α-naphthalene acetic acid (NAA), 10.00 roots for Slicass 6 at 1.0 mg/L, 6.37 leaf numbers for Slicass 11 at 0.1 mg/L 6-benzylaminopurine (BAP) and 5.6 at 1.0 and 1.5 mg/L α-naphthalene acetic acid (NAA). These observations indicate that a supplement of 0.1 mg/L 6-benzylaminopurine (BAP) in MS medium can be useful in propagation of recalcitrant cassava and low concentration of α-naphthalene acetic acid (NAA) will be beneficial in root induction prior to acclimatization with promotion in recovery of the ex vitro plants before field assessment.

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Section: Effect Of Hormones On In Vitro Root Number Per Plantlet Of Tsupporting

confidence: 92%

Development of in vitro propagation protocol for some recalcitrant cassava (Manihot esculenta Crantz) genotypes in Sierra Leone

Sesay¹,

Yamba²,

Sherman-Kamara³

et al. 2018

Afr. J. Biotechnol.

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“…Combining auxins and cytokinins, during somatic embryo maturation, is essential during cassava regeneration, as these mediate whole plant recovery (Hankoua et al, 2005;Medina et al, 2007). For instance, Fan et al (2011) demonstrated that NAA regulated organ growth; while BA facilitated cell division and elongation in cassava tuberisation. A key factor in the use of these phytohormones, is their synergistic interaction in culture media at appropriate concentrations, which ensures optimal efficiencies of regeneration.…”

Section: Discussionmentioning

confidence: 99%

“…Also, it holds potential for production of industrial starch and bioethanol (Chetty et al, 2013). Despite these values, the production and productivity of the crop is constrained by cyanogenic glycosides, post-harvest among other factors, pests and diseases (Legg and Raya, 1998;Fan et al, 2011). It is estimated that over 77,502 ha of land in Kenya are currently under cassava cultivation (FAO, 2011), yet the crop is threatened by two major diseases: cassava mosaic disease, caused by single stranded DNA geminiviruses; and the cassava brown streak disease, caused by a single stranded RNA ipomivirus (Chetty et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Optimisation of a somatic embryogenesis and transformation protocol for farmer-preferred cassava cultivars in Kenya

Marigi¹,

Masanga²,

Munga³

et al. 2016

Afr. Crop Sci. J.

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“…Tuberous root development is a complex biological process that is controlled by many internal and external factors. Phytohormones, such as auxin [ 4 , 5 ], cytokinin (CTK) [ 4 – 6 ], abscisic acid (ABA) [ 6 ], and Jasmonic acid (JA) [ 7 ], are key regulators for tuberous formation and development. Auxin and CTK in combination are crucial for tuberous initiation and formation [ 4 – 5 ], while ABA and CTK are likely the main regulation hormones for the secondary thickening growth of tuberous roots [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

Integrative Analysis of mRNA and miRNA Expression Profiles of the Tuberous Root Development at Seedling Stages in Turnips

Ding

Wang

et al. 2015

PLoS ONE

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The tuberous root of Brassica rapa L. (turnip) is an important modified organ for nutrition storage. A better understanding of the molecular mechanisms involved in the process of tuberous root development is of great value in both economic and biological context. In this study, we analyzed the expression profiles of both mRNAs and miRNAs in tuberous roots at an early stage before cortex splitting (ES), cortex splitting stage (CSS), and secondary root thickening stage (RTS) in turnip based on high-throughput sequencing technology. A large number of differentially expressed genes (DEGs) and several differentially expressed miRNAs (DEMs) were identified. Based on the DEG analysis, we propose that metabolism is the dominant pathway in both tuberous root initiation and secondary thickening process. The plant hormone signal transduction pathway may play a predominant role in regulating tuberous root initiation, while the starch and sucrose metabolism may be more important for the secondary thickening process. These hypotheses were partially supported by sequential DEM analyses. Of all DEMs, miR156a, miR157a, and miR172a exhibited relatively high expression levels, and were differentially expressed in both tuberous root initiation and the secondary thickening process with the expression profiles negatively correlated with those of their target genes. Our results suggest that these miRNAs play important roles in tuberous root development in turnips.

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Effects of Plant Growth Regulators and Saccharide on In Vitro Plant and Tuberous Root Regeneration of Cassava (Manihot esculenta Crantz)

Cited by 24 publications

References 25 publications

Development of in vitro propagation protocol for some recalcitrant cassava (Manihot esculenta Crantz) genotypes in Sierra Leone

Development of in vitro propagation protocol for some recalcitrant cassava (Manihot esculenta Crantz) genotypes in Sierra Leone

Optimisation of a somatic embryogenesis and transformation protocol for farmer-preferred cassava cultivars in Kenya

Integrative Analysis of mRNA and miRNA Expression Profiles of the Tuberous Root Development at Seedling Stages in Turnips

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