Effects of platelet-rich plasma and carbonated hydroxyapatite combination on cranial defect Bone Regeneration: An animal study

Oley, Maximillian Christian; Islam, Andi Asadul; Hatta, Mochammad; Hardjo, Marhaen; Nirmalasari, Lucia; Rendy, Leo; Ana, Ika Dewi; Bachtiar, Indra

doi:10.1016/j.wndm.2018.05.001

Cited by 10 publications

(6 citation statements)

References 7 publications

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“…The results of this study are in line with the research conducted by Oley et al (2018), who conducted an in vivo test to investigate the effects of exposure to CHA and platelet-rich plasma (PRP) on bone regeneration in the rat cranium. They found that CHA exposure did not affect bone regeneration.…”

Section: Discussionsupporting

confidence: 84%

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Effects of Carbonate Hydroxyapatite (CHA) on the Development of Heart and Cranium Cartilage of Zebrafish (Danio rerio Hamilton, 1882) Larvae

Pratama¹,

Retnoaji²,

Ana³

2022

CMUJNS

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One of the most commonly utilized materials for dental implants is carbonate hydroxyapatite (CHA). However, its usage must be free of tissue toxicity. Therefore, this study aimed to investigate how the use of CHA as a dental implant material affected the development of zebrafish (Danio rerio) embryos. CHA treatment was administered to 3–3.5 hours to 72 hours post-fertilization (hpf) embryos. Alterations in embryo and larva shape were all studied to determine toxicity. Several abnormalities that might arise during development were also examined by monitoring morphological alterations in embryos and larvae. Furthermore, heart morphology in larvae aged 72 hpf; heart rate in embryos aged 24, 48, and 72 hpf; and cardiac histology structure in larvae aged 30 dpf were all used to study heart development. Alizarin Red and Alcian Blue (ARAB) staining was employed to determine the anatomy of the cranial cartilage in larvae aged 6 dpf. Data were statistical analysis with SPSS ver. 21 and the significance was determined using one-way analysis of variance (ANOVA). The morphological examination results revealed that the embryo and larvae had no morphological abnormalities. Furthermore, the heart was developing normally, according to examinations of morphology, histology, and heart rate. The cranial cartilage had no flaws, the structure was complete, and the length and angle of the cranial cartilage did not change between the control and CHA treatments. Overall, CHA exposure did not affect the development of zebrafish (Danio rerio) embryos, according to the findings. Keywords: CHA, Cranium, Embryo, Heart, Toxicity, Zebrafish

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Section: Discussionsupporting

confidence: 84%

“…Such bioactivity related to its osteoconductivity allows the migration of osteoblasts to the surface of the material (Frayssinet et al, 1998) before directly binding to the bone. Due to these properties, CHA has been widely used in biomedical implants and bone and tooth regeneration applications (Ana et al, 2018;Oley et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Effects of Carbonate Hydroxyapatite (CHA) on the Development of Heart and Cranium Cartilage of Zebrafish (Danio rerio Hamilton, 1882) Larvae

Pratama¹,

Retnoaji²,

Ana³

2022

CMUJNS

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“…Here, further immunological features should be addressed in subsequent studies to understand the cellular background. Using a first generation PC (Platelet Rich Plasma, PRP), the combination of PC and carbonated hydroxyapatite tended to decrease pro-inflammatory cell inflammation and subsequently showed a histologically increased bone formation ( Oley et al, 2018 ).…”

Section: Discussionmentioning

confidence: 99%

Possible Implications for Improved Osteogenesis? The Combination of Platelet-Rich Fibrin With Different Bone Substitute Materials

Blatt

Thiem

Kyyak

et al. 2021

Front. Bioeng. Biotechnol.

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Bone substitute materials (BSM) are widely used in oral regeneration, but sufficient angiogenesis is crucial for osteogenesis. The combination of BSM with autologous thrombocyte concentrations such as platelet-rich fibrin (PRF) may represent a clinical approach to overcome this limitation. This study analyzes the early influence on osteoblast (HOB) in vitro. Here, four different BSM (allogeneic, alloplastic, and two of xenogeneic origin) were combined with PRF. After the incubation with osteoblasts for 24 h, cell viability, migration, and proliferation were assessed. Next, marker of proliferation, migration, and differentiation were evaluated on gene and protein levels in comparison to the native BSM and osteoblast alone. Addition of PRF increased viability for both the xenogeneic BSM (p = 0.0008, p = 0.032, respectively) in comparison to HOB and vs. native BSM (p = 0.008), and led to a tendency for increased cell proliferation and migration for all BSM (each p > 0.05). On gene basis, allogeneic and alloplastic BSM displayed a significantly increased RUNX2 expression (each p = 0.050). Expression of alkaline phosphatase for alloplastic (p = 0.050) and collagen-1 for xenogeneic BSM (p = 0.05) were significantly increased in combination with PRF. In addition, bone morphogenic protein was expressed significantly higher when xenogeneic material was combined with PRF in comparison to HOB alone (each p = 0.05). In summary, the combination of PRF with different BSM increases initial viability and may influence early proliferation and migration potential of osteoblast via RUNX2, alkaline phosphatase, collagen, and BMP2 especially in combination with alloplastic and xenogeneic BSM. Biofunctionalization of BSM using PRF might improve osteogenesis and extend the range of indications.

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“…Platelet-Rich Plasma (PRP) has been investigated in cranial bone regeneration [ 323 , 324 ]. PRP contains multiple growth factors, for instance, Platelet-Derived Growth Factor (PDGF) [ 315 , 321 ], TGF-β [ 287 , 292 ], Fibroblast Growth Factor (FGF) [ 292 , 323 ], Platelet-Derived Angiogenesis Factor (PDAF) [ 287 , 292 ], Vascular Endothelial Growth Factor (VEGF) [ 315 , 321 ], and Insulin-Like Growth Factor (IGF) [ 315 , 321 ]. PRP can be used alone, applied on a collagen sponge for sustained release [ 325 ] or encapsulated in hydrogel scaffolds, such as fibrin for craniofacial bone regeneration applications [ 305 , 306 ].…”

Section: Protein-based Therapy: Current Approaches and Potential Therapymentioning

confidence: 99%

“…Histological analysis by Xie et al show a combined treatment of PRP, bone fragments, and BMSCs show a larger area of newly formed bone tissue compared to each component used alone [ 293 ]. Similarly, Oley et al show higher lamellar bone growth when a large cranial bone defect is created in a rat animal model and scaffold with a hydroxyapatite combined with PRP is implanted [ 292 ]. These studies show efficacy of PRP as a potent growth factor regenerating bone defects.…”

Section: Protein-based Therapy: Current Approaches and Potential Therapymentioning

confidence: 99%

Craniofacial Bone Tissue Engineering: Current Approaches and Potential Therapy

Aghali

2021

Cells

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Craniofacial bone defects can result from various disorders, including congenital malformations, tumor resection, infection, severe trauma, and accidents. Successfully regenerating cranial defects is an integral step to restore craniofacial function. However, challenges managing and controlling new bone tissue formation remain. Current advances in tissue engineering and regenerative medicine use innovative techniques to address these challenges. The use of biomaterials, stromal cells, and growth factors have demonstrated promising outcomes in vitro and in vivo. Natural and synthetic bone grafts combined with Mesenchymal Stromal Cells (MSCs) and growth factors have shown encouraging results in regenerating critical-size cranial defects. One of prevalent growth factors is Bone Morphogenetic Protein-2 (BMP-2). BMP-2 is defined as a gold standard growth factor that enhances new bone formation in vitro and in vivo. Recently, emerging evidence suggested that Megakaryocytes (MKs), induced by Thrombopoietin (TPO), show an increase in osteoblast proliferation in vitro and bone mass in vivo. Furthermore, a co-culture study shows mature MKs enhance MSC survival rate while maintaining their phenotype. Therefore, MKs can provide an insight as a potential therapy offering a safe and effective approach to regenerating critical-size cranial defects.

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Effects of platelet-rich plasma and carbonated hydroxyapatite combination on cranial defect Bone Regeneration: An animal study

Cited by 10 publications

References 7 publications

Effects of Carbonate Hydroxyapatite (CHA) on the Development of Heart and Cranium Cartilage of Zebrafish (Danio rerio Hamilton, 1882) Larvae

Effects of Carbonate Hydroxyapatite (CHA) on the Development of Heart and Cranium Cartilage of Zebrafish (Danio rerio Hamilton, 1882) Larvae

Possible Implications for Improved Osteogenesis? The Combination of Platelet-Rich Fibrin With Different Bone Substitute Materials

Craniofacial Bone Tissue Engineering: Current Approaches and Potential Therapy

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