Currently in Europe, road freight transport is characterized by the most dynamic advancement. Year after year, we may observe an increase in the amount of transported goods. The paper presents the emissions of gaseous exhaust components such as CO, THC, and NOx as well as fuel consumption in freight transport. The emission analysis was performed for the entire transport cycle covering the handling of the goods with forklifts and carriage with a heavy-duty truck. The investigations were performed under actual conditions of operation using a Portable Emission Measurement System (PEMS). The fuel mileage was determined using the carbon balance method. The test routes were designed so as to reproduce the transport-logistic system typical of small towns. The setting for the tests was a town located in central Poland near the A2 motorway constituting part of the trans-European logistic network with multiple locations of logistic centers. In order to present the real emissions during handling, two test variants were considered: an outdoor variant (on a nearby lot) and inside a warehouse. The test run of the heavy-duty truck involved transporting 24,000 kg of load on urban and extra-urban (local and intercity) roads. The exhaust emissions and fuel mileage were determined for each of the stages as well as for the entire research cycle.