Effects of Polyvalent Cobalt Salts on Human Cells in Tissue Culture

Lazzarini, Abel A.; Weissmann, Gerald

doi:10.1126/science.131.3415.1736

Cited by 9 publications

(1 citation statement)

References 4 publications

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“…The precipitate was washed 3 times each with absolute ethanol and ethyl ether and dried in vacuo over CaCI2. Judged by its metachromasia with toluidine blue, and precipitability by polyvalent cobalt salts (13), this product was presumed to contain water soluble acid mucopolysaccharide-protein complexes. Chemical analysis of 10 samples of this fraction (Fraction A, Figure 1) (Figure 1) yielded upon chemical analysis the following, as per cent: hexosamine 2.4 + 0.6, nitrogen 13.1 + 0.8, hydroxyproline 6.9 + 0.5.…”

Section: Methodsmentioning

confidence: 99%

X-Ray Diffraction Studies of Human Aortic Elastin Residues*

Weissmann

Weissmann²

1960

J. Clin. Invest.

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Section: Methodsmentioning

confidence: 99%

X-Ray Diffraction Studies of Human Aortic Elastin Residues*

Weissmann

Weissmann²

1960

J. Clin. Invest.

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The biologic activity of polyanions: Past history and new prospectives

Regelson

1979

J. polym. sci., C Polym. symp.

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SYNOPSISThe toxicology of synthetic polyanions relates to molecular weight. This developed from experience with pyran copolymer (divinyl ether maleic anhydride copolymer), which was tested clinically as an antitumor agent and found to be too toxic for continued clinical trial. Synthetic polyanions of low molecular weight are potent stimuli to macrophage phagocytic function and possess antitumor activity. Increased host resistance to pathogens including bacteria, fungi, viruses, and trypanosomes is seen. In contrast to lower-molecular-weight fractions, higher-molecular-weight samples inhibit macrophage function, and apparently increased molecular weight > 15,000 is necessary for antiviral and immunologic stimulating capacity. One possible mechanism for the action of polyanions may relate to redistribution of calcium ions within the cell and increasing cyclic GMP activity. This is a redefinition for the role of polyanions in sol -gel alteration in cytoplasm. The killing effect of the polyanion-activated macrophage on tumor cells resembles that of the polyanion on the nucleus. Polyanions may be potent controllers of the stability of DNA within the nucleus and can act to derepress cells to turn on protein synthesis or, alternatively, inhibit enzymes involved in virus synthesis and control of RNA or DNA polymerase. Thus, they are cogent tools in an understanding of the physiology of nuclear interaction. Biological use for these agents can be developed not only in clinical approaches to inhibition of tumor or viral infection, but to eliminate plutonium, and provide selective enzyme inhibition and protein interaction which allows for isolation, neutralization, and characterization of blood and tissue protein and glycoprotein fractions vital for a wide range of pathophysiology.(1,000,ooO M.W.) Sulf. Chitoran Sulf. Pectic Acid AmidsSulf. Oxid. Cellulose Hydroxornic Acid Laminorin Sulfate aAll animals showed 10% body weight loss. Legend: +, % inhibition 50% or greater; (+), 30-49%. b leukemia [48, 59-621, C3H mammary tumor transplants [62], methylcholanthrene derived transplants [63], and dimethylbenzanthracene (DMBA) induced tumors [64], the LSTRA sarcoma [58], Madison lung carcinoma [65] and prostatic adenocarcinoma in the rat [66], and the M109 lung carcinoma and colon tumor [67]. Pyran prevents polyoma induced tumors and Rauscher leukemia in thymectomized mice [57]. Pyran induces interferon in both humans and mice [16 -18, 52, 681, but antitumor and antiviral activity do not correlate with interferon induction [25, 54, 691. Pyran can both stimulate and inhibit host immune response [l, 2, 25, 70 -761 and alter in vitro lymphoblastic response to lipopolysaccharide (LPS) or phytohemagglutinin (PHA) [70 -741. Pyran inhibits the early cation-dependent steps of complement activity (G. S. White, personal communication, 1976) [77, 781, but can activate the C3 bypass. Pyran can prevent adjuvant induced arthritis [76], and its effects on skin graft survival are controversial. Toxicologically, the clinically tested pyran fraction...

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Diarthrodial joints, an essay

Hamerman¹,

Schubert²

1962

The American Journal of Medicine

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Effects of Polyvalent Cobalt Salts on Human Cells in Tissue Culture

Cited by 9 publications

References 4 publications

X-Ray Diffraction Studies of Human Aortic Elastin Residues*

X-Ray Diffraction Studies of Human Aortic Elastin Residues*

The biologic activity of polyanions: Past history and new prospectives

Diarthrodial joints, an essay

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