Effects of Porcine Follicular Fluid and Oviduct-Conditioned Media on Maturation and Fertilization of Porcine Oocytes In Vitro1

Vatzias, Georgios; Hagen, D. R.

doi:10.1095/biolreprod60.1.42

Cited by 74 publications

(37 citation statements)

References 43 publications

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“…This difference may be at the basis of the observed discrepancy. In fact, Vatzias & Hagen (1999) postulated the possibility that follicular fluid could contain not yet completely identified factors exerting a positive effect on oocyte maturation, in the same time exerting a protective role against exogenous insults during IVM. To answer this question, we elicit to expose porcine COCs to bNF in the absence of follicular fluid.…”

Section: Discussionmentioning

confidence: 99%

Regulation of aryl hydrocarbon receptor activity in porcine cumulus–oocyte complexes in physiological and toxicological conditions: the role of follicular fluid

Nestler¹,

Risch²,

Fischer³

et al. 2007

Reproduction

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The arylhydrocarbon receptor (AhR) mediates the adverse effects of dioxin-like compounds. However, it has also been reported that the AhR may exert a role in ovarian physiology. In the present study, porcine cumulus-oocyte complexes (COCs) were matured in vitro in the presence of 10% follicular fluid. Expression of AhR and its partner, AhR nuclear translocator occurs in immature COCs. After in vitro maturation (IVM), an up-regulation of AhR and cytochrome P450 1A1 (CYP1A1; the main AhRtarget gene) was observed. To explore the role of the AhR during IVM, we exposed the COCs to 50 mM b-napthoflavone (bNF). The treatment induced a marked up-regulation of CYP1A1 mRNA, indicating both constitutive and inducible AhR activity. However, in contrast to what was observed in other cell types, no sign of toxicity was observed in COCs. To investigate if components of porcine follicular fluid may exert a protective role against AhR ligands, we exposed porcine COCs to bNF, in the absence of follicular fluid. In these conditions, a marked decrease in the percentage of matured oocytes, concomitant with an increase in oocyte degeneration, was observed. Furthermore, bNF increased apoptosis in cumulus cells in the absence of follicular fluid, whereas bNF has no effects when COCs were treated in the presence of porcine follicular fluid (pFF). In conclusion, these results suggest the presence of unknown endogenous AhR-ligand(s) during porcine IVM and that a dysregulation of this mechanism may result in ovotoxicity by inducing apoptosis in cumulus cells. However, this phenomenon is interrupted by the presence of follicular fluid, indicating a putative protective role for follicular fluid components against exogenous insults.

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Section: Discussionmentioning

confidence: 99%

Regulation of aryl hydrocarbon receptor activity in porcine cumulus–oocyte complexes in physiological and toxicological conditions: the role of follicular fluid

Nestler¹,

Risch²,

Fischer³

et al. 2007

Reproduction

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“…Moreover, recent findings in pigs [5,25] and horses [16] have shown that the co-culture with oviductal epithelial cells during in vitro maturation does not affect nuclear maturation rates but positively influences in vitro embryo development. Thus it is possible that co-culture with oviductal epithelial cells during maturation enhances cytoplasmic maturation in oocytes and thereby contributes to its potential for embryo development.…”

Section: Effect Of Co-culture On Ivm Of Canine Oocytesmentioning

confidence: 98%

Influence of co-culture with oviductal epithelial cells on in vitro maturation of canine oocytes

et al. 2002

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-The process of oocyte maturation in the canine species is unique among mammals: oocytes are immature at ovulation and the resumption and progression of meiotic maturation occur in the oviduct. This study was performed to investigate (i) the effect of co-culture with infundibulum and ampullar oviductal epithelial cells on the in vitro maturation of canine oocytes and (ii) the culture time necessary to reach full meiotic maturation. For this purpose the oocytes, collected from the ovaries of bitches undergoing ovariectomies, were divided into three groups and cultured for 48 and 72 h with the following systems: (A) TCM 199 + 10% oestrus bitch serum + FSH (0.1 IU . mL -1 ), LH (0.1 IU . mL -1 )+ progesterone (1 mg . mL -1 ) + oestradiol (1 mg.mL -1 ) + cysteamine (100 mM); (B) medium A plus infundibulum cells; (C) medium A plus ampullar cells. Infundibulum and ampullar cells were recovered from the oviducts of bitches at the oestrus stage of their cycle. The results showed that after 48 h of incubation, a significantly higher meiotic resumption (P < 0.01) was observed in the oocytes cultured with infundibulum (59%) and ampullar cells (60.0%), than in the control group (40.0%). There was also a significantly (P < 0.01) higher meiotic progression to the MII in systems B and C (15.6% and 16.7%) than in system A (4.0%). After 72 h of culture, the percentages of meiotic resumption and progression were unchanged. These results showed that both the infundibulum and the ampullar oviductal epithelial cells positively influence the meiotic resumption and progression of canine oocytes and that 48 h are sufficient for the completion of nuclear maturation. bitch / oocyte / in vitro maturation / oviductal cells / oestrous cycle Reprod. Nutr. Dev. 42 (2002) 265-273 265

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“…It seems counterintuitive to include follicular fluid to encourage oocyte maturation, as it contains oocyte maturation inhibitors, such as hypoxanthine, which arrest oocyte meiosis (30)(31)(32). Nevertheless, the beneficial effects of using follicular fluid during IVM in many studies (33)(34)(35)(36) provided clues that its content of growth factors could be beneficial for oocyte maturation. Here, we provide evidence that three cytokines, fibroblast growth factor 2 (FGF2), leukemia inhibitory factor (LIF), and insulin-like growth factor 1 (IGF1), each known to be present in follicular fluid (37)(38)(39)(40), when used in combination can provide much improved oocyte competence, most probably by influencing the timing of MAPK1/3 activation in the cumulus cells enveloping the oocyte.…”

Section: Significancementioning

confidence: 99%

Quadrupling efficiency in production of genetically modified pigs through improved oocyte maturation

Yuan

Spate

Redel

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

117

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Assisted reproductive technologies in all mammals are critically dependent on the quality of the oocytes used to produce embryos. For reasons not fully clear, oocytes matured in vitro tend to be much less competent to become fertilized, advance to the blastocyst stage, and give rise to live young than their in vivo-produced counterparts, particularly if they are derived from immature females. Here we show that a chemically defined maturation medium supplemented with three cytokines (FGF2, LIF, and IGF1) in combination, so-called "FLI medium," improves nuclear maturation of oocytes in cumulus-oocyte complexes derived from immature pig ovaries and provides a twofold increase in the efficiency of blastocyst production after in vitro fertilization. Transfer of such blastocysts to recipient females doubles mean litter size to about nine piglets per litter. Maturation of oocytes in FLI medium, therefore, effectively provides a fourfold increase in piglets born per oocyte collected. As they progress in culture, the FLI-matured cumulus-oocyte complexes display distinctly different kinetics of MAPK activation in the cumulus cells, much increased cumulus cell expansion, and an accelerated severance of cytoplasmic projections between the cumulus cells outside the zona pellucida and the oocyte within. These events likely underpin the improvement in oocyte quality achieved by using the FLI medium.cumulus cell | embryo development | genetic modification | in vitro fertilization | MAPK signaling

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Effects of Porcine Follicular Fluid and Oviduct-Conditioned Media on Maturation and Fertilization of Porcine Oocytes In Vitro1

Cited by 74 publications

References 43 publications

Regulation of aryl hydrocarbon receptor activity in porcine cumulus–oocyte complexes in physiological and toxicological conditions: the role of follicular fluid

Regulation of aryl hydrocarbon receptor activity in porcine cumulus–oocyte complexes in physiological and toxicological conditions: the role of follicular fluid

Influence of co-culture with oviductal epithelial cells on in vitro maturation of canine oocytes

Quadrupling efficiency in production of genetically modified pigs through improved oocyte maturation

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