Effects of porcine granulocyte-macrophage colony-stimulating factor on porcine in vitro-fertilized embryos

Kwak, Seong-Sung; Jeung, S.H.; Biswas, Dibyendu; Jeon, Yubyeol; Hyun, Sang Hwan

doi:10.1016/j.theriogenology.2011.10.025

Cited by 40 publications

(34 citation statements)

References 49 publications

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“…In previous research, GM-CSF inhibited the cellular stress response and apoptosis pathways to facilitate embryo growth and survival [10]. In bovine [13] and porcine [12,26] embryos, GM-CSF also increases the percentage of embryos that develop to the blastocyst stage in vitro.…”

mentioning

confidence: 86%

“…Oocyte collection and in vitro maturation: Retrieval and IVM of porcine oocytes from local slaughterhouse-derived ovaries were performed as we described previously [4,26]. Briefly, cumulus oocyte complexes (COCs) were aspirated using an 18-gauge needle attached to a 10 ml disposable syringe from superficial follicles 3 to 6 mm in diameters, pooled in 15 ml conical tubes and allowed to settle down as sediment for 5 min at 37°C.…”

Section: Chemicalsmentioning

confidence: 99%

“…GM-CSF is expressed during early pregnancy in epithelial cells lining the oviduct and uterus in humans [9], rodents [37] and other mammalian species [15,32]. It enhances the development of preimplantation embryos in vitro and increases total cell numbers in the embryos of humans [41], mice [38], cows [13] and pigs [12,26]. Human embryos exposed to recombinant human GM-CSF in vitro blastulate earlier, have increased cell numbers in both the inner cell mass (ICM) and trophectoderm (TE) with reduced apoptosis [42] and show improved ability to hatch and attach to the culture dish [41].…”

mentioning

confidence: 99%

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Porcine Granulocyte-Macrophage Colony-Stimulating Factor Improves the <i>in vitro</i> Development of Cloned Porcine Embryos

Kwak

Cheong

Jeon

et al. 2012

The Journal of Veterinary Medical Science

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ABSTRACT. We examined the effects of porcine granulocyte-macrophage colony-stimulating factor (pGM-CSF) on the in vitro development of porcine embryos produced by somatic cell nuclear transfer (SCNT) for the first time. We evaluated the effects of pGM-CSF on SCNTderived blastocyst formation and investigated gene expression. A total of 522 cloned embryos in 6 replicates were treated with 10 ng/ml pGM-CSF during in vitro culture (IVC). This treatment significantly (P<0.05) increased blastocyst formation and total cell number in blastocysts compared with the control (12.3% and 41.4 vs. 9.0% and 34.7, respectively). However, there was no effect on cleavage rate. The numbers of cells in the inner cell mass and trophectoderm were significantly higher in the pGM-CSF treatment group (6.0 and 43.0, respectively) compared with the control (4.4 and 31.9, respectively). Treatment with 10 ng/ml pGM-CSF significantly increased POU5F1 and Cdx2 mRNA expression in blastocysts. In addition, Bcl-2, Dnmt1 and proliferating cell nuclear antigen (PCNA) mRNA expression were upregulated in blastocysts in the pGM-CSF supplemented group compared with the control. These results suggest that pGM-CSF improves the quality and developmental viability of porcine SCNT embryos by regulating transcription factor expression.

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mentioning

confidence: 86%

Section: Chemicalsmentioning

confidence: 99%

mentioning

confidence: 99%

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Porcine Granulocyte-Macrophage Colony-Stimulating Factor Improves the <i>in vitro</i> Development of Cloned Porcine Embryos

Kwak

Cheong

Jeon

et al. 2012

The Journal of Veterinary Medical Science

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“…Although advancement in in vitro culture medium has greatly improved the efficacy of assisted reproductive technologies in the treatment of infertility, there is still disparity between in vitro culture and in vivo conditions [1][2][3][4]. Culture conditions are important for the outcome of assisted reproductive technologies and have an impact on pre-and postimplantation development and possibly the future health of the offspring [1,[5][6][7][8].…”

Section: Introductionmentioning

confidence: 99%

“…An important optimizing protocol is a supplementation of some embryokines to the culture medium [1][2][3][4][11][12][13][14][15]. Among various embryokines, granulocyte-macrophage colony-stimulating factor (GM-CSF) has attracted much attention.…”

Section: Introductionmentioning

confidence: 99%

Effects of granulocyte-macrophage colony-stimulating factor supplementation in culture medium on embryo quality and pregnancy outcome of women aged over 35 years

Zhou

Chu

Sha

et al. 2015

J Assist Reprod Genet

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Purpose The purpose of this study is to explore whether a low concentration of granulocyte-macrophage colony-stimulating factor (GM-CSF) supplementation in culture medium is beneficial to infertile women aged over 35 years. Conclusion Although no benefit of GM-CSF on embryo quality was observed, the addition of this factor significantly decreased the occurrence of chemical pregnancy of women aged over 35 years, indicating the role of GM-CSF in improving implantation competence of embryos derived from elderly infertile women.

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Piglets produced from cloned blastocysts cultured in vitro with GM‐CSF

Lee

Redel

Spate

et al. 2013

Molecular Reproduction Devel

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SUMMARY In general, pig embryos established by somatic cell nuclear transfer (SCNT) are transferred at the one-cell stage because of suboptimal embryo culture conditions. Improvements in embryo culture can increase the practical application of late embryo transfer. The goal of this study was to evaluate embryos cultured with granulocyte-macrophage colony-stimulating factor (GM-CSF) in vitro, and to track the in vivo developmental competency of SCNT-derived blastocysts from these GM-CSF embryos. The receptor for GM-CSF was up-regulated in in vitro-produced embryos when compared to in vivo-produced cohorts, but the level decreased when GM-CSF was present. In vitro fertilized (IVF) embryos, supplemented with GM-CSF (2 or 10 ng/ml), showed a higher frequency of development to the blastocyst stage compared to controls. The total cell numbers of the blastocysts also increased with supplementation of GM-CSF. Molecular analysis demonstrates that IVF-derived blastocysts cultured with GM-CSF exhibit less apoptotic activity. Similarly, an increase in development to the blastocyst stage and an increase in the average total-cell number in the blastocysts were observed when SCNT-derived embryos were cultured with either concentration of GM-CSF (2 or 10 ng/ml). When SCNT-derived embryos, cultured with 10 ng/ml GM-CSF, were transferred into six surrogates at Day 6, five of the surrogates became pregnant and delivered healthy piglets. Our findings suggest that supplementation of GM-CSF can provide better culture conditions for IVF- and SCNT-derived embryos, and pig SCNT-derived embryos cultured with GM-CSF in vitro can successfully produce piglets when transferred into surrogates at the blastocyst stage. Thus, it may be practical to begin performing SCNT-derived embryo transfer at the blastocyst stage.

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Effects of porcine granulocyte-macrophage colony-stimulating factor on porcine in vitro-fertilized embryos

Cited by 40 publications

References 49 publications

Porcine Granulocyte-Macrophage Colony-Stimulating Factor Improves the <i>in vitro</i> Development of Cloned Porcine Embryos

Porcine Granulocyte-Macrophage Colony-Stimulating Factor Improves the <i>in vitro</i> Development of Cloned Porcine Embryos

Effects of granulocyte-macrophage colony-stimulating factor supplementation in culture medium on embryo quality and pregnancy outcome of women aged over 35 years

Piglets produced from cloned blastocysts cultured in vitro with GM‐CSF

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