Effects of PPAR-γ Agonist Treatment on LPS-Induced Mastitis in Rats

Abstract

PPAR-γ, a member of the nuclear receptor superfamily, plays an important role in lipid metabolism and inflammation. The aim of this study was to investigate the preventive effects of synthetic PPAR-γ agonist rosiglitazone on lipopolysaccharide (LPS)-induced mastitis in rats. The mouse model of mastitis was induced by the injection of LPS through the duct of the mammary gland. Rosiglitazone was injected 1 h before the induction of LPS intraperitoneally. The results showed that rosiglitazone attenuated the infiltration of inflammatory cells, the activity of myeloperoxidase (MPO), and the production of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and interleukin-1β (IL-1β) in a dose-dependent manner. Additionally, Western blotting showed that rosiglitazone inhibited the phosphorylation of IκB-α and NF-κB p65. These results indicated that rosiglitazone has a protective effect on mastitis, and the anti-inflammatory mechanism of rosiglitazone on LPS-induced mastitis in rats may be due to its ability to inhibit NF-κB signaling pathways. PPAR-γ may be a potential therapeutic target against mastitis.

1,8-Cineole Ameliorates LPS-Induced Vascular Endothelium Dysfunction in Mice via PPAR-γ Dependent Regulation of NF-κB

“…It is also known that PPAR-γ activation leads to the repression of pro-inflammatory genes such as cyclooxygenase 2, inducible nitric oxide synthase and interleukin (IL)-6, and the reduction of cytokines and acute phase proteins by inhibiting the NF-κB pathway (Yang et al, 2014). Ultimately, inhibition of NF-κB by PPAR-γ agonists were reported to reduce the generation of pro-inflammatory mediators/responses (Mingfeng et al, 2014; Marcone et al, 2015; Su et al, 2017).…”

Lactoferrin suppresses lipopolysaccharide-induced endometritis in mice via down-regulation of the NF-κB pathway

“…Gram-negative bacteria, such as Escherichia coli, Arcanobacterium pyogenes, Fusobacterium necrophorum and Prevotella melaninogenicus, are recognized as major pathogens associated with uterine endometrial lesions [5,6]. LPS, a main component of the outer membrane of gram-negative bacteria, is an important factor in induced inflammatory responses in cultured cells and live animals [7,8]. Currently, the main drugs of treatment for endometritis involve antibiotics, disinfectants, preservatives and hormones.…”

Ameliorative effect of rosiglitazone, a peroxisome proliferator gamma agonist on adriamycin‐induced cardio toxicity via suppressing oxidative stress and apoptosis

“…Rosiglitazone (RSG) is an antidiabetic agent that belongs to the thiazolidinediones, and is a nuclear hormone receptor peroxisome proliferator gamma (PPARγ) agonist. 6,7 These receptors express most abundantly in fat tissue where they assume the main role in metabolism of lipid and adipogenesis. 8,9 Through actuating nuclear receptors PPARγ, RSG can control the glucose production and transportation, and furthermore direct the translation of the insulin response genes.…”

“…Rosiglitazone (RSG) is an antidiabetic agent that belongs to the thiazolidinediones, and is a nuclear hormone receptor peroxisome proliferator gamma (PPARγ) agonist . These receptors express most abundantly in fat tissue where they assume the main role in metabolism of lipid and adipogenesis .…”