Effects of preparations from the skin of the Arabian Gulf catfish (Arius bilineatus Val.) on back pain and other related pain

Al-Hassan, Jassim M.; Ramadan, Abbas; Khan, Reyadh; Abdel-Razek, Mamdouh; Thomson, Martha; Ali, Muslim

doi:10.1096/fasebj.21.5.a241

Cited by 8 publications

(20 citation statements)

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“…Sheep red blood cells also showed variability similar to that noted for human type O cells. Several studies have convincingly established that the repeated application of preparations involving catfish epidermal secretions to wounds and persistent diabetic foot ulcers in human subjects had a positive effect on wound healing (Al‐Hassan, 1990; Al‐Hassan et al., 1991). The complex mixture of components contained in the epidermal secretion obviously induces a balanced stimulation of the early stages of wound healing.…”

Section: Discussionmentioning

confidence: 99%

“…Their functions are not well defined, but some protective roles have been suggested (Cameron and Endean, 1973; Al‐Hassan et al., 1985). Several studies also provided evidence that preparations from the skin secretions of A. bilineatus can stimulate the rate of wound healing in animals and healing of diabetic foot ulcers in humans (Al‐Hassan et al.,1983,1985,1987a,b, 1991; Al‐Hassan, 1990).…”

Section: Introductionmentioning

confidence: 99%

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Histochemical Analysis of Glycoconjugates in the Skin of a Catfish (Arius Tenuispinis, Day)

Al-Banaw

Kenngott

Al-Hassan

et al. 2010

Anat Histol Embryol

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Summary A histochemical study using conventional carbohydrate histochemistry (periodic‐acid staining including diastase controls, alcian blue staining at pH 1 and 2.5) as well as using a battery of 14 fluorescein isothiocyanate (FITC)‐labelled lectins to identify glycoconjugates present in 10 different areas of the skin of a catfish (Arius tenuispinis) was carried out. The lectins used were: mannose‐binding lectins (Con A, LCA and PSA), galactose‐binding lectins (PNA, RCA), N‐acetylgalactosamine‐binding lectins (DBA, SBA, SJA and GSL I), N‐acetylglucosamine‐binding lectins (WGA and WGAs), fucose‐binding lectins (UEA) and lectins which bind to complex carbohydrate configurations (PHA E, PHA L). Conventional glycoconjugate staining (PAS staining, alcian blue at pH 1 and 2.5) showed that the mucous goblet cells contain a considerable amount of glycoconjugates in all locations of the skin, whereas the other unicellular gland type, the club cells, lacked these glycoconjugates. The glycoproteins found in goblet cells are neutral and therefore stain magenta when subjected to PAS staining. Alcian blue staining indicating acid glycoproteins was distinctly positive at pH 1, but gave only a comparable staining at pH 2.5. The mucus of the goblet cells therefore also contains acid glycoproteins rich in sulphate groups. Using FITC‐labelled lectins, the carbohydrate composition of the glycoproteins of goblet cells could be more fully characterized. A distinct staining of the mucus of goblet cells was found with the mannose‐binding lectins LCA and PSA; the galactosamine‐binding lectins DBA, SBA and GLS I; the glucosamine‐binding lectin WGA; and PHA E which stains glycoproteins with complex carbohydrate configurations. No reaction occurred with the fucose‐binding lectin UEA and the sialic acid‐specific lectin SNA. In addition, the galactose‐binding lectins PNA and RCA showed only a weak or completely negative staining of the mucus in the goblet cells. The specificity of the lectin staining could be proved by inhibiting binding of the lectins by competitive inhibition with the corresponding sugars. From these data, we can conclude that the mucus produced by the epidermal goblet cells of A. tenuispinis is rich in mannose, N‐acetylgalactosamine and N‐acetylglucosamine residues.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Histochemical Analysis of Glycoconjugates in the Skin of a Catfish (Arius Tenuispinis, Day)

Al-Banaw

Kenngott

Al-Hassan

et al. 2010

Anat Histol Embryol

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“…Application of fish mucus on mammalian wounds may activate healing (Al‐Hassan et al . 1983; Al‐Hassan 1990; Al‐Hassan et al . 1991; Akunne et al .…”

Section: The Anatomy and Functions Of Fish Skinmentioning

confidence: 99%

Mechanical induced wounds in fish – a review on models and healing mechanisms

Sveen

Karlsen

Ytteborg

2020

Reviews in Aquaculture

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Skin wounds and injuries are frequently occurring in farmed fish, particularly in more intensive production systems. Any disruptions of the skin, such as mucus removal, scale loss or deeper incisions, are negatively correlated with barrier functions and disease resistance. In this review, the current knowledge on mechanically induced wounds in fish is dedicated to five areas of focus: (i) The anatomy and functions of fish skin, (ii) Techniques to inflict mechanical skin damage in farmed and laboratory fish for experimental purposes, (iii) Healing mechanisms of deep wounds, (iv) In vitro models for wound healing studies and (v) Wound care, with focus on factors that may enhance or delay skin regenerative processes. The aim of the review was to presents key points for a better understanding of skin resilience and fish robustness, with focus on Atlantic salmon, Salmo salar, in Norwegian coastal production environment.

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“…These fish that are often wounded by marine predators, developed the unique mechanism by which they are able to effectively heal their wounds (Al-Hassan et al, 1985). This curious observation initiated a series of studies directed by one of us [JA-H] (Al-Hassan et al, 1983;Al-Hassan et al, 1991) that established PCEGS preparations could accelerate wound healing (Al-Hassan et al, 1983;Al-Hassan et al, 1991), and were found effective in treating human chronic diabetic foot ulcers that were unresponsive to conventional therapy (Al-Hassan, 1990). Biochemical analysis has shown that the PCEGS preparations, used in the initial studies, contained several biologically active proteins, including growth factors (Al-Hassan et al, 1986;Al-Lahham et al, 1987;Thomson et al, 1989) and lipids (Al-Hassan et al, 1986;Plattner et al, 1988;Summers et al, 1991;Al-Hassan et al, 1998;Khan et al, 2018;Al-Hassan et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Potential Mechanism of Dermal Wound Treatment With Preparations From the Skin Gel of Arabian Gulf Catfish: A Unique Furan Fatty Acid (F6) and Cholesta-3,5-Diene (S5) Recruit Neutrophils and Fibroblasts to Promote Wound Healing

et al. 2020

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Preparations from Arabian Gulf catfish (Arius bilineatus, Val) epidermal gel secretion (PCEGS) effectively heal chronic wounds in diabetic patients. However, specific lipid components of PCEGS that are responsible for various aspects of wound healing are unknown. Here, we report for the first time that, i) a unique preparation containing only proteins and lipids (Fraction B, FB), derived from the PCEGS accelerated the healing of experimental dermal wounds in female rats (transdermal punch biopsy) in vivo. Histological analyses showed that topical treatment of these wounds with FB promoted the migration of fibroblasts, facilitated the production of extracellular matrix (collagen, fibronectin), induced capillary formation and recruitment of immune cells, and accelerated overall wound healing by day 4 (tested at 1, 2, 3, 4, and 10 days; n=15 for vehicle; n=15 for FB treatment), ii) the lipids responsible for different stages of wound healing were separated into a protein-free bioactive lipid fraction, Ft, which contained a few common long-chain fatty acids, a unique furan fatty acid (F6) and a cholesterol metabolite, cholesta-3,5-diene (S5). Ft (the partially purified lipid fraction of PCEGS), and F6 and S5 present in Ft, proved to be bioactive for wound healing in human dermal fibroblasts. Ft increased the

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Effects of preparations from the skin of the Arabian Gulf catfish (Arius bilineatus Val.) on back pain and other related pain

Cited by 8 publications

References 0 publications

Histochemical Analysis of Glycoconjugates in the Skin of a Catfish (Arius Tenuispinis, Day)

Histochemical Analysis of Glycoconjugates in the Skin of a Catfish (Arius Tenuispinis, Day)

Mechanical induced wounds in fish – a review on models and healing mechanisms

Potential Mechanism of Dermal Wound Treatment With Preparations From the Skin Gel of Arabian Gulf Catfish: A Unique Furan Fatty Acid (F6) and Cholesta-3,5-Diene (S5) Recruit Neutrophils and Fibroblasts to Promote Wound Healing

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