Effects of preparative parameters on the properties of chitosan hydrogel beads containing Candida rugosa lipase

Alsarra, Ibrahim A.; Neau, Steven H.; Howard, Matthew A.

doi:10.1016/j.biomaterials.2003.09.051

Cited by 66 publications

(36 citation statements)

References 25 publications

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“…These values are comparable to the results obtained in some related studies concerning protein entrapment in hydrogels (Chen et al, 2005). On the other hand, the encapsulation efficiency achieved in the present work are higher than those previously reported in the literature for protein incorporated in various hydrogels based on natural polymers such as alginate (Nochos et al, 2008;Simi and Abraham, 2007) or chitosan (Alsarra et al, 2004).…”

Section: Lipase Loading In Hydrogelssupporting

confidence: 89%

Hydrogels of N-isopropylacrylamide copolymers with controlled release of a model protein

Milašinović¹,

Krušić²,

Knežević‐Jugović³

et al. 2010

International Journal of Pharmaceutics

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Section: Lipase Loading In Hydrogelssupporting

confidence: 89%

Hydrogels of N-isopropylacrylamide copolymers with controlled release of a model protein

Milašinović¹,

Krušić²,

Knežević‐Jugović³

et al. 2010

International Journal of Pharmaceutics

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“…One explanation is that high TPP mass at fixed QC concentration may cause a rise in solution pH value, with a consequential effect on increased overall negative surface charge carried by the protein molecules, which enhanced electrostatic interactions between QC and BSA molecules. [27] In fact, the pH value for QC-4 solutions (2 mg Á mL À1 ) slightly increased from 7.9 to 8.1 as the TPP concentration increased from 0.6 to 0.8 mg Á mL À1 , and the pH value for QC-6 solutions (2 mg Á mL À1 ) increased from 7.7 to 8.0 as the TPP concentration increased from 0.7 to 0.9 mg Á mL À1 , respectively. In summary, the incorporation of increase in amounts of BSA led to an enlargement of the QC nanoparticles, a decrease on their surface charge, an enhancement of their LE and LC.…”

Section: Resultsmentioning

confidence: 99%

Preparation and Characterization of Novel Quaternized Cellulose Nanoparticles as Protein Carriers

Song

Zhou

et al. 2009

Macromolecular Bioscience

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Quaternized cellulose (QC) nanoparticles were prepared in distilled water by ionic crosslinking of QC with sodium tripolyphosphate (TPP) for the first time. BSA as a model protein drug was used to investigate the loading and release features of the nanoparticles. The results indicated that QC nanoparticles had high loading efficiency and capacity for BSA. The in vitro BSA release of the QC nanoparticles displayed a burst effect in the first 2 h and then a slow continuous release. Nanoparticles with a higher DS of QC showed a decrease in particle size, an increase in zeta potential, a higher loading efficiency and a slower drug-release profile. These studies demonstrated that QC nanoparticles are potential protein carriers, and that their physicochemical properties and release profile could be easily adjusted.

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“…17 Since the candidates (including enzymes, peptides, and proteins) for such hydrogel systems can be very expensive, low entrapment efficiency would cause a waste and limit the use of such systems. The entrapment efficiencies of a number of enzymes in some hydrogel systems have been found to be 25-45%.…”

Section: Entrapment Efficiency Of Bsa In Gel Beadsmentioning

confidence: 99%

Calcium‐carboxymethyl chitosan hydrogel beads for protein drug delivery system

Liu

Jiao

Zhang

2006

J of Applied Polymer Sci

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In this study, carboxymethyl chitosan (CMC) hydrogel beads were prepared by crosslinking with Ca 2þ . The pH-sensitive characteristics of the beads were investigated by simulating gastrointestinal pH conditions. As a potential protein drug delivery system, the beads were loaded with a model protein (bovine serum albumin, BSA). To improve the entrapment efficiency of BSA, the beads were further coated with a chitosan/CMC polyelectrolyte complex (PEC) membrane by extruding a CMC/BSA solution into a CaCl 2 /chitosan gelation medium. Finally, the release studies of BSA-loaded beads were conducted. We found that, the maximum swelling ratios of the beads at pH 7.4 (17-21) were much higher than those at pH 1.2 (2-2.5). Higher entrapment efficiency (73.2%) was achieved in the chitosan-coated calcium-CMC beads, compared with that (44.4%) in the bare calcium-CMC beads. The PEC membrane limited the BSA release, while the final disintegration of beads at pH 7.4 still leaded to a full BSA release. Therefore, the chitosan-coated calcium-CMC hydrogel beads with higher entrapment efficiency and proper protein release properties were a promising protein drug carrier for the site-specific release in the intestine.

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Effects of preparative parameters on the properties of chitosan hydrogel beads containing Candida rugosa lipase

Cited by 66 publications

References 25 publications

Hydrogels of N-isopropylacrylamide copolymers with controlled release of a model protein

Hydrogels of N-isopropylacrylamide copolymers with controlled release of a model protein

Preparation and Characterization of Novel Quaternized Cellulose Nanoparticles as Protein Carriers

Calcium‐carboxymethyl chitosan hydrogel beads for protein drug delivery system

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