Effects of pro-inflammatory cytokines on chondrogenesis of equine mesenchymal stromal cells derived from bone marrow or synovial fluid

Zayed, Mohammed; Schumacher, Jim; Misk, N. A.; Dhar, Madhu

doi:10.1016/j.tvjl.2016.05.014

Cited by 29 publications

(33 citation statements)

References 53 publications

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“…Besides, the cells carry limited potential to produce glycosaminoglycans (Zayed, Schumacher, Misk, & Dhar, 2016). Thus, to avoid such adverse effects of the inflammation, drugs to reduce the same may be used.…”

Section: Osteoarthritismentioning

confidence: 99%

Mesenchymal stem cell basic research and applications in dog medicine

Gugjoo

Amarpal

Sharma

2019

Journal Cellular Physiology

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The stem cells, owing to their special characteristics like self‐renewal, multiplication, homing, immunomodulation, anti‐inflammatory, and dedifferentiation are considered to carry an “all‐in‐one‐solution” for diverse clinical problems. However, the limited understanding of cellular physiology currently limits their definitive therapeutic use. Among various stem cell types, currently mesenchymal stem cells are extensively studied for dog clinical applications owing to their readily available sources, easy harvesting, and ability to differentiate both into mesodermal, as well as extramesodermal tissues. The isolated, culture expanded, and characterized cells have been applied both at preclinical as well as clinical settings in dogs with variable but mostly positive results. The results, though positive, are currently inconclusive and demands further intensive research on the properties and their dependence on the applications. Further, numerous clinical conditions of dog resemble to that of human counterparts and thus, if proved rewarding in the former may act as basis of therapy for the latter. The current review throws some light on dog mesenchymal stem cell properties and their potential therapeutic applications.

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Section: Osteoarthritismentioning

confidence: 99%

Mesenchymal stem cell basic research and applications in dog medicine

Gugjoo

Amarpal

Sharma

2019

Journal Cellular Physiology

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“…anti-microbial properties [59]), but might at the same time be involved in catabolic pathways [60]. Moreover, the leukocyte fraction in L-PRF has been reported to be accountable for the overproduction of several growth factors, including VEGF and inflammatory cytokines [59,61], which have been described to negatively impact chondrogenesis in vitro [31,[62][63][64][65][66][67]. In contrast, many other growth factors present in L-PRF ex and L-PRF CM are reported to have beneficial influences on MSC chondrogenesis [31,34,68,69].…”

Section: Discussionmentioning

confidence: 99%

Therapeutic Potential of Dental Pulp Stem Cells and Leukocyte- and Platelet-Rich Fibrin for Osteoarthritis

Monaco

Gervois

Beaumont

et al. 2020

Cells

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Osteoarthritis (OA) is a degenerative and inflammatory joint disorder with cartilage loss. Dental pulp stem cells (DPSCs) can undergo chondrogenic differentiation and secrete growth factors associated with tissue repair and immunomodulation. Leukocyte-and platelet-rich fibrin (L-PRF) emerges in regenerative medicine because of its growth factor content and fibrin matrix. This study evaluates the therapeutic application of DPSCs and L-PRF in OA via immunomodulation and cartilage regeneration. Chondrogenic differentiation of DPSCs, with or without L-PRF exudate (ex) and conditioned medium (CM), and of bone marrow-mesenchymal stem cells was compared. These cells showed differential chondrogenesis. L-PRF was unable to increase cartilage-associated components. Immature murine articular chondrocytes (iMACs) were cultured with L-PRF ex, L-PRF CM, or DPSC CM. L-PRF CM had pro-survival and proliferative effects on unstimulated and cytokine-stimulated iMACs. L-PRF CM stimulated the release of IL-6 and PGE2, and increased MMP-13, TIMP-1 and IL-6 mRNA levels in cytokine-stimulated iMACs. DPSC CM increased the survival and proliferation of unstimulated iMACs. In cytokine-stimulated iMACs, DPSC CM increased TIMP-1 gene expression, whereas it inhibited nitrite release in 3D culture. We showed promising effects of DPSCs in an in vitro OA model, as they undergo chondrogenesis in vitro, stimulate the survival of chondrocytes and have immunomodulatory effects.

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“…However, the use of xenogenic MSCs is currently not recommended in clinical trials. MSCs have anti-inflammatory properties but high-end inflammation usually reduces their ability for chondrogenic differentiation without affecting their phenotypic characteristics and proliferation potential (Ando et al 2012;Zayed et al 2016). In equine OA, the limited efficacy of their MSCs may be explained by an increased expression of the adhesion molecule, a decrease in the migration of related genes (Barrachina et al 2016;Reesink et al 2017), and the production of glycosaminoglycans (Zayed et al 2016).…”

Section: In Vivo Preclinical Experimental Models/ Clinical Studiesmentioning

confidence: 99%

“…MSCs have anti-inflammatory properties but high-end inflammation usually reduces their ability for chondrogenic differentiation without affecting their phenotypic characteristics and proliferation potential (Ando et al 2012;Zayed et al 2016). In equine OA, the limited efficacy of their MSCs may be explained by an increased expression of the adhesion molecule, a decrease in the migration of related genes (Barrachina et al 2016;Reesink et al 2017), and the production of glycosaminoglycans (Zayed et al 2016). Pro-inflammatory cytokines like IL-1b, IL-17, and TNF-a decrease the expression levels of cartilage-specific genes like SOX-9 and TGF-b1, and those encoding aggrecan, collagen II (Kondo et al 2013;Zayed et al 2016), and galactin (Reesink et al 2017) in the MSCs.…”

Section: In Vivo Preclinical Experimental Models/ Clinical Studiesmentioning

confidence: 99%

Animal mesenchymal stem cell research in cartilage regenerative medicine – a review

Gugjoo

Amarpal

Fazili

et al. 2019

Veterinary Quarterly

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Healing of articular cartilage is a major clinical challenge as it also lacks a direct vasculature and nerves, and carries a limited number of resident chondrocytes that do not proliferate easily. Damaged articular cartilages are usually replaced by fibrocartilages, which are mechanically and structurally weaker and less resilient. Regenerative medicine involving stem cells is considered to have a definitive potential to overcome the limitations associated with the currently available surgical methods of cartilage repair. Among various stem cell types, mesenchymal stem cells (MSCs) are preferred for clinical applications. These cells can be readily derived from various sources and have the ability to trans-differentiate into various tissue-specific cells, including those of the cartilage by the process of chondrogenesis. Compared to embryonic or induced pluripotent stem cells (iPSCs), no ethical or teratogenic issues are associated with MSCs. These stem cells are being extensively evaluated for the treatment of joint affections and the results appear promising. Unlike human medicine, in veterinary medicine, the literature on stem cell research for cartilage regeneration is limited. This review, therefore, aims to comprehensively discuss the available literature and pinpoint the achievements and limitations associated with the use of MSCs for articular cartilage repair in animal species.

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Effects of pro-inflammatory cytokines on chondrogenesis of equine mesenchymal stromal cells derived from bone marrow or synovial fluid

Cited by 29 publications

References 53 publications

Mesenchymal stem cell basic research and applications in dog medicine

Mesenchymal stem cell basic research and applications in dog medicine

Therapeutic Potential of Dental Pulp Stem Cells and Leukocyte- and Platelet-Rich Fibrin for Osteoarthritis

Animal mesenchymal stem cell research in cartilage regenerative medicine – a review

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