Effects of progesterone and estradiol benzoate on superoxide dismutase activity in the brain of male rats

Pajović, Snežana B.; Saičić, Zorica S.; Spasić, Mihajlo; Petrović, Vojislav M.; Martinović, Jelena

doi:10.1007/bf01920710

Cited by 24 publications

(28 citation statements)

References 15 publications

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“…We found no differences in levels of SOD1 protein expression between males and females for either brain region. Similarly, SOD1 activity in cytosolic fractions of whole brain homogenates was not affected by gonadectomy or sex hormone therapy (Pajović et al, 1993(Pajović et al, , 1996. Thus, it appears that sexassociated differences in ischemic cerebral susceptibility are not mediated by different basal expression levels of SOD1.…”

Section: Discussionmentioning

confidence: 92%

SOD1 Overexpression and Female Sex Exhibit Region-Specific Neuroprotection after Global Cerebral Ischemia Due to Cardiac Arrest

Kofler

Hurn

Traystman

2005

J Cereb Blood Flow Metab

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Cardiac arrest is often associated with poor neurologic outcome since therapeutic options are limited. We tested the hypothesis that overexpression of CuZn superoxide dismutase (SOD þ /À) is neuroprotective in a new murine model of cardiac arrest and cardiopulmonary resuscitation (CPR). Second, we investigated if female and male mice sustain similar injury and if sex-specific outcomes are altered by SOD overexpression. Neuronal injury was quantified 3 days after 8 mins of KClinduced cardiac arrest by calculating the percentage of ischemic neurons for caudoputamen and hippocampal CA1 region. In rostral caudoputamen, less neuronal cell loss was found for SOD þ /À mice (31%722%) when compared with wild-type (WT) mice (47%731%, Po0.05). Superoxide dismutase overexpression did not reduce injury in the caudal caudoputamen. No sex-linked protection was evident in either genotype in the caudoputamen. Female WT mice had less CA1 injury than male WT mice (26%731% versus 54%730%, Po0.05), whereas no sex difference was found in SOD þ /À mice (female: 42%729%; male: 37%737%). Comparison of hippocampal injury between genotypes revealed no differences for either males or females. In conclusion, SOD1 overexpression and female sex were associated with significant neuroprotection in this murine cardiac arrest model. However, no additive neuroprotection was observed, and these beneficial effects were restricted to specific brain regions.

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Section: Discussionmentioning

confidence: 92%

SOD1 Overexpression and Female Sex Exhibit Region-Specific Neuroprotection after Global Cerebral Ischemia Due to Cardiac Arrest

Kofler

Hurn

Traystman

2005

J Cereb Blood Flow Metab

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“…Yet, we found no data concerning hormonal modulation of antioxidant enzyme activities during developmental processes. Our previous studies have shown that estradiol benzoate (EB) and progesterone modulate the activities of SOD, GPx, CAT, and glutathione reductase in brain of adult rats (16)(17)(18). The inhibition of brain mitochondrial manganese-SOD (MnSOD) activity by EB without affecting cytosol copper,zinc-SOD (CuZnSOD) activity was a response of rats of both sexes.…”

Section: Introductionmentioning

confidence: 99%

The modulatory effect of estradiol benzoate on superoxide dismutase activity in the developing rat brain

Pejić

Kasapović

Cvetković

et al. 2003

Braz J Med Biol Res

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The sensitivity of copper,zinc (CuZn)-and manganese (Mn)-superoxide dismutase (SOD) to exogenous estradiol benzoate (EB) was investigated in Wistar rats during postnatal brain development. Enzyme activities were measured in samples prepared from brains of rats of both sexes and various ages between 0 and 75 days, treated sc with 0.5 µg EB/100 g body weight in 0.1 ml olive oil/100 g body weight, 48 and 24 h before sacrifice. In females, EB treatment stimulated MnSOD activity on days 0 (66.1%), 8 (72.7%) and 15 (81.7%). In males, the stimulatory effect of EB on MnSOD activity on day 0 (113.6%) disappeared on day 8 and on days 15 and 45 it became inhibitory (40.3 and 30.5%, respectively). EB had no effect on the other age groups. The stimulatory effect of EB on CuZnSOD activity in newborn females (51.8%) changed to an inhibitory effect on day 8 (38.4%) and disappeared by day 45 when inhibition was detected again (48.7%). In males, the inhibitory effect on this enzyme was observed on days 0 (45.0%) and 15 (28.9%), and then disappeared until day 60 when a stimulatory effect was observed (38.4%). EB treatment had no effect on the other age groups. The sensitivity of MnSOD to estradiol differed significantly between sexes during the neonatal and prepubertal period, whereas it followed a similar pattern thereafter. The sensitivity of CuZnSOD to estradiol differed significantly between sexes during most of the study period. Regression analysis showed that the sensitivity of MnSOD to this estrogen tended to decrease similarly in both sexes, whereas the sensitivity of CuZnSOD showed a significantly different opposite tendency in female and male rats. These are the first reports indicating hormonal modulation of antioxidant enzyme activities related to the developmental process. Correspondence

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“…The antioxidant effect of estrogen has been regarded as the main mechanism for this hormone to protect skeletal and cardiac muscles (12), uterus (13), and liver (9) from damage. In brain, progesterone instead of estrogen exerts a significant antioxidant effect (14). Contrary to female steroids, testosterone has been shown to decrease the activities of SOD, CAT, and GSH-Px, leading to lipid peroxidation (15).…”

mentioning

confidence: 99%

Regulation of antioxidant enzyme activities in male and female rat macrophages by sex steroids

Azevedo

Lacava

Miyasaka

et al. 2001

Braz J Med Biol Res

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Human and animal immune functions present sex dimorphism that seems to be mainly regulated by sex hormones. In the present study, the activities of the antioxidant enzymes total superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) were measured in intraperitoneal resident macrophages from adult male and female rats. In addition to comparing males and females, we also examined the regulation of these enzyme activities in macrophages by sex steroids. GSH-Px activity did not differ between male and female macrophages. However, both total SOD and CAT activities were markedly higher in females than in males (83 and 180%). Removal of the gonads in both males and females (comparison between castrated groups) increased the difference in SOD activity from 83 to 138% and reduced the difference in CAT activity from 180 to 86%. Castration and testosterone administration did not significantly modify the activities of the antioxidant enzymes in male macrophages. Ovariectomy did not affect SOD or GSH-Px activity but markedly reduced (48%) CAT activity. This latter change was fully reversed by estrogen administration, whereas progesterone had a smaller effect. These results led us to conclude that differences in the SOD and CAT activities may partially explain some of the differences in immune function reported for males and females. Also, estrogen is a potent regulator of CAT in macrophages and therefore this enzyme activity in macrophages may vary considerably during the menstrual cycle.

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Effects of progesterone and estradiol benzoate on superoxide dismutase activity in the brain of male rats

Cited by 24 publications

References 15 publications

SOD1 Overexpression and Female Sex Exhibit Region-Specific Neuroprotection after Global Cerebral Ischemia Due to Cardiac Arrest

SOD1 Overexpression and Female Sex Exhibit Region-Specific Neuroprotection after Global Cerebral Ischemia Due to Cardiac Arrest

The modulatory effect of estradiol benzoate on superoxide dismutase activity in the developing rat brain

Regulation of antioxidant enzyme activities in male and female rat macrophages by sex steroids

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