Effects of prolonged fasting on levels of metabolites, oxidative stress, immune-related gene expression, histopathology, and DNA damage in the liver and muscle tissues of rainbow trout (Oncorhynchus mykiss)

Starvation Affects the Muscular Morphology, Antioxidant Enzyme Activity, Expression of Lipid Metabolism-Related Genes, and Transcriptomic Profile of Javelin Goby (Synechogobius hasta)

“…Synechogobius hasta. Similar to the muscle morphological alterations reported in several fish species under food deprivation [40,42,43], the histological appearance of muscle was evidently affected after 7 and 14 days of starvation (Figure 4), with muscle tissues exhibiting larger interlobular intervals and atrophy of some muscle trabeculae. Moreover, these findings were consistent with the dynamic decline in the myofiber dimension of starved S. hasta with extended fasting time (Figure 4).…”

“…Moreover, these findings were consistent with the dynamic decline in the myofiber dimension of starved S. hasta with extended fasting time ( Figure 4 ). Given that decreases in energy anabolism and increases in catabolism have been confirmed to weaken muscle function and myofiber activity, resulting in a reduction in muscle size [ 42 , 44 ], the abnormal muscle structure in S. hasta subjected to more than 7 days of fasting was suggestive of a reduced biosynthesis of endogenous reserves, impaired contractility, and growth retardation in S. hasta muscle. This assumption was partly supported by the transcriptional expression of lipid metabolism-related genes observed in our study.…”

Exploring the impacts of different fasting and refeeding regimes on Nile tilapia (Oreochromis niloticus L.): growth performance, histopathological study, and expression levels of some muscle growth-related genes

“…Several studies reported that fasting caused liver degeneration, decreased hepatocyte size, and enlargement of the sinus in Chanos Chanos juveniles , Amphiprion melanopus , and green sturgeon ( Acipenser medirostris ) (Storch and Juario 1983 ; Green and McCormick 1999 ). Moreover, steatosis, inflammation, degeneration, necrosis, and hyperemia with reduced liver tissue were all observed in rainbow trout ( Oncorhynchus mykiss ) (Karatas et al 2021 ), which could be related to impaired lipid transport, lipid biosynthesis, or both (Colakoglu and Donmez 2012 ), although the elevated blood flow, inflammation, necrosis, and enlargement of arterioles were all documented to be suggested reasons of hyperemia (Karataş et al 2019a , b ).…”

Potencial Antioxidante Em Tilápias Submetidas a Desafio Sanitário E Nutricional Simultâneo

“…Uma forma eficiente de avaliar o dano à proteína de animais sob condições de estresse oxidativo, é a avaliação de proteína carbonilada (Buss et al, 1997), que estará elevada quando o dano for causado. Em nosso estudo, a maior concentração de proteína carbonilada foi encontrada no fígado de peixes submetidos ao jejum intermitente, e uma vez que o fígado é um potencial alvo dos efeitos da relacionado ao estresse energético (Karatas et al 2021) 1997). A mensuração das enzimas antioxidantes tem sido realizada na tentativa de decifrar os eventos fisiológicos associados a esse processo, pois a atividade dessas enzimas muda em resposta ao estresse oxidativo (ABDALLA, 2015).…”