This research was designed to determine the effects of rosemary (Rosmarinus officinalis) extract on growth, blood biochemistry, immunity, antioxidant, digestive enzymes and liver histopathology of rainbow trout. In a 40‐day diet experiment, rainbow trout was fed with the experimental diets containing 0.4, 0.7, 1 and 3 g/kg of rosemary. 0.4 and 0.7 g/kg rosemary extracts had no significant effect on all parameters studied (p > .05). The growth performance (SGR and WG) and feed utilization (FCR and FER) of the fish increased with the increase in the rate of rosemary in the feed (p < .001). Additionally, only the final weight levels were significant at the linear level (p < .05). Rosemary's 1 and 3 g/kg doses increased total protein, trypsin, amylase, lipase, superoxide dismutase (SOD), catalase (CAT), lysozyme, total immunoglobulin (IG) and white blood cell (WBC) levels when compared to control group. However, glucose, aspartate aminotransferase (AST), alanine aminotransferase (ALT), total cholesterol and triglyceride levels decreased significantly (p < .001). Moreover, these doses of rosemary caused a significant reduction in steatosis of liver hepatocytes compared to the control group. In conclusion, the present study showed that rosemary may be useful in reducing the stress effects by increasing the growth, antioxidant, digestive enzymes and immunity levels, as well as reducing the rate of steatosis in the liver of rainbow trout.
This study was to assess the effects of deltamethrin on some haematological parameters of brown trout (Salmo trutta fario). During the 4 day, 10 brown trout were exposed to two different concentrations of Deltamethrin which they were 20% (0.91 μg·L-1) and 40% (1.88 μg·L-1) respectively, (LC50=4.7 μg·L-1). The results obtained from this study showed that the White Blood Cell (WBC), Hemoglobin content (Hb), Hematocrit percentage (PCV), Mean Corpuscular Volume (MCV) and Mean Corpuscular Hemoglobin (MCH) were decreased in two treated groups when compared to the control (p
This study was conducted to investigate the effects of starvation for 8 days on the serum metabolites and antioxidant enzymes, thiobarbituric acid reacting substance levels, endogenous reserves in liver and muscle tissues of rainbow trout (Oncorhynchus mykiss). Eight-days of fasting caused a significant decrease in glucose, total protein, triglyceride, cholesterol, high-density lipoprotein and low-density lipoprotein levels as well as protein and lipid reserves in liver and muscle tissue of fish (p<0.05). The fasting period had no significant effect on the hepatic thiobarbituric acid reactive substances (TBARS), but, it caused a significant decrease in the hepatic catalase and glutathione peroxidase (p<0.05). In muscle tissue, the TBARS levels were significantly decreased (p<0.05). Whereas, antioxidant enzyme activities remained unchanged (p>0.05). The results obtained from this study showed that the short-term starvation leads to significant changes on serum metabolites, antioxidant enyzme activities and endogenous reserves of rainbow trout.
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