Effects of Propofol on Electrocardiogram Measures in Mice

Abstract

Abstract.We investigated the anesthetic effects of propofol on the electrocardiogram (ECG) in mice. We also compared the effects of isoflurane (2%) inhalation anesthesia, intraperitoneal propofol (50 or 100 mg/kg), and pentobarbital (50 mg/kg) on ECG in mice. Isoflurane inhalation and pentobarbital anesthesia were both associated with an acceptable heart rate (HR) range (ca. 450 -500 bpm). In contrast, high-dose propofol anesthesia significantly decreased the HR. Importantly, propofol anesthesia led to significantly reduced responses to propranolol, a b-blocker, suggesting that it affects sympathetic tonus and is not suitable for the evaluation of cardiovascular or sympathetic function. Propofol also reduced the response to atropine, indicative of suppression of mouse parasympathetic nerve activity. Our data suggest that propofol anesthesia should not be the first choice for cardiovascular analysis in mice.

Non-invasive ECG recording and QT interval correction assessment in anesthetized rats and mice

“…Mice mean HR of the lineages study was about 450bpm, similar to previous studies (Shintaku et al 2014). It is noteworthy that HR is affected by the type of anesthesia used in all rodent species.…”

“…PR interval is also affected by anesthesia and was reported in Wistar rats to be 39 to 78ms and of 35.7±0.9ms in mice (Shintaku et al 2014). The present study revealed similar mean values of 34.4 for rats and 35.7 for mice.…”

Nanoformulation of Premixing Propofol Lipid Emulsion and Fentanyl Citrate and Their Effects on Acute Toxicity, Sedation, and Analgesia

“…The absence of particles >5 m might explain why the LD 50 values and HE-stained histopathological test results did not significantly change in these two administration methods. Furthermore, the cardiovascular or respiratory depression might be the main reason for death [18,19]. The intravenous injection LD 50 values of propofol and fentanyl were 53 and 11.2 mg/kg in mice according to the drug instructions, respectively.…”

“…In the main test for each group, one female mouse was randomly selected and intravenously administered with a preliminary dose of 17.84 mg/kg propofol + 8.92 g/kg fentanyl. Single animals were dosed in sequence at 1 h intervals for the rapid cardiovascular and respiratory depression of propofol [18,19]. The subsequent ascending or descending doses were determined depending on the survival from the preceding dose of the animals with a dose progression factor of 1.25.…”

Rifaximin, a pregnane X receptor (PXR) activator regulates apoptosis in a murine model of breast cancer

“…Following the same, time and frequency domain parameters of HRV were calculated using the Lab chart Pro-8 (AD Instruments, Australia). 19 Carmine staining of whole mounts mammary gland Mammary gland tissues from each group were assessed for their morphological changes using carmine staining. The tissues were xed by putting the slides into Carney's xative solution for two days.…”