Effects of Prostaglandin E
            <sub>2</sub>
            and Progesterone on Rat Brain Synaptosomal Plasma Membranes

Deliconstantinos, George

doi:10.1002/9780470513989.ch11

Ciba Foundation Symposium 153 ‐ Steroids and Neuronal Activity

2007

DOI: 10.1002/9780470513989.ch11

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Effects of Prostaglandin E ₂ and Progesterone on Rat Brain Synaptosomal Plasma Membranes

George Deliconstantinos

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2008

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“…Namely, progesterone increases nerve conduction speed by reducing Na + /K + ATPase [20], and high estrogen and progesterone levels post ovulation induce Th2 anti-inflammatory cytokines [21]. Consequently, a quick drop of such hormones in premenstrual periods might cause transitory worsening of pre-existing symptoms or trigger clinical exacerbations via a Th2/Th1 shift [22].…”

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confidence: 98%

Female-specific issues in multiple sclerosis

Ghezzi¹,

Zaffaroni

2008

Expert Review of Neurotherapeutics

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Complex neuroendocrine mechanisms regulating the immune response can be recognized in all autoimmune diseases. Such mechanisms develop through endocrine loops and feedback processes along the hypothalamus-pituitary-gonads axis and the hypothalamus-pituitary- adrenal gland axis. Females are not only more susceptible to autoimmune diseases, but are also more exposed to relevant variations of hormonal levels that physiologically go along with women's life. This paper reviews female-specific issues in multiple sclerosis and how treatments must be considered accordingly. In particular, aspects related to puberty, menses, fertility, pregnancy, lactation and menopause are considered in addition to epidemiological and clinical issues.

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mentioning

confidence: 98%

Female-specific issues in multiple sclerosis

Ghezzi¹,

Zaffaroni

2008

Expert Review of Neurotherapeutics

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Met-enkephalin receptor-mediated increase of membrane fluidity modulates nitric oxide (NO) and cGMP production in rat brain synaptosomes

1995

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The association of [3H]-Met-enkephalin with synaptosomes isolated from rat brain cortex, when incubated for 30 min at 25 degrees C follows a sigmoid path with a Hill coefficient h = 1.25 +/- 0.04. Binding of Met-enkephalin into synaptosomes was saturable, with an apparent binding constant of 8.33 +/- 0.48 nM. At saturation, Met-enkephalin specific receptors corresponded to 65.5 +/- 7.2 nmol/mg synaptosomal protein. The Hill plot in combination with the biphasic nature of the curve to obtain the equilibrium constant, showed a moderate degree of positive cooperativity in the binding of Met-enkephalin into synaptosomes of at least one class of high affinity specific receptors. Met-enkephalin increased the lipid fluidity of synaptosomal membranes labelled with 1,6-diphenyl-1,3,5-hexatriene (DPH), as indicated by the steady-state fluorescence anisotropy [(ro/r)-1]-1. Arrhenius-type plots of [(ro/r)-1]-1 indicated that the lipid separation of the synaptosomal membranes at 23.4 +/- 1.2 degrees C was perturbed by Met-enkephalin such that the temperature was reduced to 15.8 +/- 0.8 degrees C. Naloxone reversed the fluidizing effect of Met-enkephalin, consistent with the receptor-mediated modulation of membrane fluidity. Naloxone alone had no effect on membrane fluidity. NO release and cGMP production by NO-synthase (NOS) and soluble guanylate cyclase (sGC), both located in the soluble fraction of synaptosomes (synaptosol) were decreased by 82% and 80% respectively, after treatment of synaptosomes with Met-enkephalin (10(-10)-10(-4) M). These effects were reversed by naloxone (10(-4) M) which alone was ineffective in changing NO and cGMP production.(ABSTRACT TRUNCATED AT 250 WORDS)

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Effects of Prostaglandin E ₂ and Progesterone on Rat Brain Synaptosomal Plasma Membranes

Cited by 2 publications

References 32 publications

Female-specific issues in multiple sclerosis

Female-specific issues in multiple sclerosis

Met-enkephalin receptor-mediated increase of membrane fluidity modulates nitric oxide (NO) and cGMP production in rat brain synaptosomes

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Effects of Prostaglandin E 2 and Progesterone on Rat Brain Synaptosomal Plasma Membranes

Cited by 2 publications

References 32 publications

Female-specific issues in multiple sclerosis

Female-specific issues in multiple sclerosis

Met-enkephalin receptor-mediated increase of membrane fluidity modulates nitric oxide (NO) and cGMP production in rat brain synaptosomes

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Effects of Prostaglandin E ₂ and Progesterone on Rat Brain Synaptosomal Plasma Membranes