Effects of prostaglandin E2 and D2 on cell proliferation and osteogenic capacity of human mesenchymal stem cells

Ern, Christina; Frasheri, Iris; Berger, T.; Kirchner, H.; Heym, Richard; Hickel, Reinhard; Folwaczny, Matthias

doi:10.1016/j.plefa.2019.09.005

Cited by 4 publications

(7 citation statements)

References 41 publications

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“…Further researches are needed to elucidate the mechanisms and speci c roles played by IGFBP2 in promoting osteogenesis or adipogenesis in MSCs. PTGER2 acts as a main receptor of prostaglandin E2, which has been shown to encourage osteogenic differentiation of MSCs, and stimulate the resorption and formation of bones (20)(21)(22). It is suggested in several current studies that local application of PTGER2 agonists to fractured rats in vitro can effectively stimulate the formation of bone marrow and periosteum, thus promoting fracture repairing (23)(24)(25).…”

Section: Discussionmentioning

confidence: 99%

Identification of Biomarkers Modulating Osteogenic Differentiation With Immune and Inflammation Landscapes in Mesenchymal Stem Cells: A Bioinformatics-based Comprehensive Study

Feng

Wang

et al. 2022

Preprint

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Background: Inducing osteogenic mesenchymal stem cell (MSC) differentiation brings a huge potential for clinical bone defect repair. This present study is aimed to identify novel key biomarkers for osteogenesis of MSCs, and analyze their possible regulation roles on immune and inflammation during this process.Results: Seven datasets (GSE159137, GSE159138, GSE114117, GSE88865, GSE153829, GSE63754, GSE73087) were obtained from the gene expression omnibus database, and 200 differentially expressed genes (DEGs) were identified from the train datasets. Biomarkers were screened through Logical regression of the selection operator algorithm, whose expressions and diagnostic performances were verified in the test datasets. FKBP prolyl isomerase 5 (FKBP5), insulin like growth factor binding protein 2, prostaglandin E receptor 2, SAM and HD domain containing deoxynucleoside triphosphate triphosphohydrolase 1 (SAMHD1) and transmembrane O-mannosyltransferase targeting cadherins 1 were established to be osteogenesis-related biomarkers. Finally, the DEGs of immune and inflammation in osteogenesis were detected, and their correlation with the hub biomarkers were analyzed. The five selected biomarkers are strongly associated with immune and inflammation related genes.Conclusion: Taken together, our manuscript identified five biomarkers related to stem cells osteogenesis and discussed their potential perspectives in immunoregulation, inflammation and tissue engineering during the process.

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Section: Discussionmentioning

confidence: 99%

Identification of Biomarkers Modulating Osteogenic Differentiation With Immune and Inflammation Landscapes in Mesenchymal Stem Cells: A Bioinformatics-based Comprehensive Study

Feng

Wang

et al. 2022

Preprint

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“…Lipid metabolism plays a pivotal role in stem cells physiopathology [ 108 , 109 , 110 ]. However, at the moment the number of studies about the lipidome of MSCs is limited, and mainly focused on variations in lipid composition during stem cell proliferation and differentiation [ 111 , 112 , 113 , 114 , 115 , 116 , 117 , 118 , 119 , 120 , 121 , 122 , 123 , 124 , 125 , 126 , 127 , 128 , 129 , 130 ] ( Figure 2 ).…”

Section: Bioactive Lipids In Mscsmentioning

confidence: 99%

“…In particular, PGE 2 increases human umbilical cord blood-derived MSCs (UCMSCs) proliferation through β-catenin-mediated c-Myc and vascular endothelial growth factor expression via exchange protein directly activated by cAMP (Epac1)/Ras-related protein 1 (Rap1)/Akt and PKA cooperation [ 116 ], and through interaction of profilin-1 (Pfn-1) and filamentous-actin (F-actin) via EP2 receptor-dependent β-arrestin-1/JNK signaling pathways [ 117 ]. On the contrary, the investigation of PGE 2 and prostaglandin D2 (PGD 2 ) effects on MSCs proliferation and osteogenic differentiation suggests that both their receptors are highly expressed in these cells and both prostaglandins seem to have a negative impact [ 118 ]. In detail, PGE 2 firstly enhances the MSCs growth-rate, while longer stimulation leads to a growth-inhibitory effect.…”

Section: Bioactive Lipids In Mscsmentioning

confidence: 99%

“…Contrarily, PGD 2 inhibits MSCs growth regardless of the duration of the exposure. Moreover, their inhibitory effect on calcium deposition also suggests a negative impact on MSCs osteogenic differentiation [ 118 ]. Moreover, TXs class has been investigated for its effect on MSCs proliferation, suggesting the role of TXA 2 as potent modulator of ASCs migration and proliferation through ERK and p38 MAPK signaling mechanisms [ 119 ].…”

Section: Bioactive Lipids In Mscsmentioning

confidence: 99%

“…In the presence of PGE 2 , also a higher expression of EP3, which is involved in the stimulation of angiogenesis, was obtained in MSCs suggesting a possible correlation with the early phases of inflammation [ 118 ].…”

Section: Bioactive Lipids In Mscsmentioning

confidence: 99%

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Bioactive Lipids in MSCs Biology: State of the Art and Role in Inflammation

Casati

Giannasi

Niada

et al. 2021

IJMS

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Lipidomics is a lipid-targeted metabolomics approach that aims to the comprehensive analysis of lipids in biological systems in order to highlight the specific functions of lipid species in health and disease. Lipids play pivotal roles as they are major structural components of the cellular membranes and energy storage molecules but also, as most recently shown, they act as functional and regulatory components of intra- and intercellular signaling. Herein, emphasis is given to the recently highlighted roles of specific bioactive lipids species, as polyunsaturated fatty acids (PUFA)-derived mediators (generally known as eicosanoids), endocannabinoids (eCBs), and lysophospholipids (LPLs), and their involvement in the mesenchymal stem cells (MSCs)-related inflammatory scenario. Indeed, MSCs are a heterogenous population of multipotent cells that have attracted much attention for their potential in regulating inflammation, immunomodulatory capabilities, and reparative roles. The lipidomics of the inflammatory disease osteoarthritis (OA) and the influence of MSCs-derived lipids have also been addressed.

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New Perspectives on the Associations between Blood Fatty Acids, Growth Parameters, and Cognitive Development in Global Child Populations

et al. 2023

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Malnutrition is prevalent in low-middle-income countries (LMICs), but it is usually clinically diagnosed through abnormal anthropometric parameters characteristic of protein energy malnutrition (PEM). In doing so, other contributors or byproducts of malnutrition, notably essential fatty acid deficiency (EFAD), are overlooked. Previous research performed mainly in high-income countries (HICs) shows that deficiencies in essential fatty acids (EFAs) and their n-3 and n-6 polyunsaturated fatty acid (PUFA) byproducts (also known as highly unsaturated fatty acids or HUFAs) lead to both abnormal linear growth and impaired cognitive development. These adverse developmental outcomes remain an important public health issue in LMICs. To identify EFAD before severe malnutrition develops, clinicians should perform blood fatty acid panels to measure levels of fatty acids associated with EFAD, notably Mead acid and HUFAs. This review demonstrates the importance of measuring endogenous fatty acid levels for measuring fatty acid intake in various child populations in LMICs. Featured topics include a comparison of fatty acid levels between global child populations, the relationships between growth and cognition and PUFAs and the possible mechanisms driving these relationships, and the potential importance of EFAD and HUFA scores as biomarkers of overall health and normal development.

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Effects of prostaglandin E2 and D2 on cell proliferation and osteogenic capacity of human mesenchymal stem cells

Cited by 4 publications

References 41 publications

Identification of Biomarkers Modulating Osteogenic Differentiation With Immune and Inflammation Landscapes in Mesenchymal Stem Cells: A Bioinformatics-based Comprehensive Study

Identification of Biomarkers Modulating Osteogenic Differentiation With Immune and Inflammation Landscapes in Mesenchymal Stem Cells: A Bioinformatics-based Comprehensive Study

Bioactive Lipids in MSCs Biology: State of the Art and Role in Inflammation

New Perspectives on the Associations between Blood Fatty Acids, Growth Parameters, and Cognitive Development in Global Child Populations

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