Effects of prostaglandin F2 treatment on semen characteristics of crossbred rams in the non-breeding season

Abstract

The aim of the present study was to evaluate the effects of administration of PGF 2 α on semen characteristics in crossbred rams in the non-breeding season. Twenty crossbred rams (Arkhar-Merino × Moghani and Arkhar-Merino × Ghezel) were randomly allocated into two equal groups. The experimental group received 7.5 mg IM of Estroplan (PGF2α analogue) and the control group received 1 ml of water soluble. A total number of eighteen ejaculates per ram were collected by artificial vagina twice a week 30 min following IM administration. Semen ejaculates were evaluated for volume, sperm concentration, total sperm output, mass and individual motility, methylene blue reduction time-test (MBRT), percentage of live sperm and sperm abnormality rates. The results of the current study shows that PGF 2 α treatment on crossbreed rams improved most semen characteristics including volume, sperm concentration, total sperm output and MBRT in comparison to control group (P < 0.01). However, other semen characteristics show similar values. We concluded that PGF 2 α actually improved semen output without any negative effect on sperm qualities.

Concentration and motility of spermatozoa and testosterone level of kacang goat after seminal vesicle extract administration

“…Decreased motility after administration of PGF2α not only occurs in kacang goat but could also occur in other animals. This is in accordance with the statement of Capitan et al (1990), Masoumi et al (2011), and Olfati et al (2013). Capitan et al (1990) reported the motility of buffalo spermatozoa pretreatment, in treatment, and post-treatment were 43.19%; 45.46%; and 49.06%.…”

“…Failure in increasing spermatozoa concentrations in this study was likely due to the long interval (two days) between PGF2α administration and spermatozoa collection. In other studies, semen collection was carried out 30 minutes after PGF2α administration significantly improve the spermatozoa concentration (Kreider et al, 1981;Hashizume and Niwa, 1984;Mekonnen et al, 1989;Estienne and Harper, 2004;Olfati et al, 2013). However, in study conducted by Capitan et al (1990) who performed semen collection one hour after PGF2α administration and seven days after the last PGF2α injection (post treatment) did not able to increase spermatozoa concentration.…”

“…Masoumi et al (2011) reported that mean spermatozoa motility induced with PGF2α vs control were 53.9±1.6 vs 51.9±1.6% and 36.1±0.6 vs 35.6±0.7%, respectively. Olfati et al (2013) reported a comparison of sheep spermatozoa motility between groups given PGF2α with controls were 67.8±11.3 and 65.1±10.6%, respectively. Decline in sperm motility after PGF2α administration was not only observed in vivo but also reported in vitro.…”

The IMPROVEMENT OF SEMEN QUALITY AND TESTOSTERONE LEVEL OF BALI CATTLE AFTER PROSTAGLANDIN F2α ADMINISTRATION

“…The optimal interval between prostaglandin PGF2α injection and ejaculation is around 30-60 minutes. This confirmed the report of Olfati et al (2013) which concluded that the best administration interval was 30 minutes while Capitan et al (1990) showed that some semen characteristics of the treatment group and posttreatment group (1 mg prostaglandin PGF2α) increased significantly at 1 hour compared to the control group.…”

The Pengaruh Pemberian PGF2α terhadap Peningkatan Kualitas Spermatozoa Kambing Boerka

“…Interval antar perlakuan adalah satu minggu. Koleksi semen dilakukan 30 menit setelah penyuntikan PGF2α sesuai petunjuk Olfati et al (2013). Pola penyuntikan sampel disajikan pada Tabel 1.…”