Effects of Protease-resistant Antimicrobial Substances Produced by Lactic Acid Bacteria on Rumen Methanogenesis

Asa, Reina; Tanaka, Akihiro; Uehara, Akiko; Shinzato, I.; Toride, Yasuhiko; Usui, N.; Hirakawa, Kozue; Takahashi, J.

doi:10.5713/ajas.2010.90444

Cited by 19 publications

(13 citation statements)

References 38 publications

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“…The effect of the LP80 supernatant in combination with P. productus was also studied in vivo using two rams and it was concluded that inhibition of methanogenesis (80% decrease; mmol/6 h) occurred during the first 3 days but the effect did not persist. Compounds (PRA1) produced by L. plantarum TUA1490L were tested in vitro and found to decrease methanogenesis by 90% (Asa et al, 2010). Further work with PRA1 confirmed its ability to maintain an antimicrobial effect even after incubation with proteases but the hypothesis that the inhibition mechanism of PRA1 may relate to the production of hydrogen peroxide has not been proven (Takahashi, 2013).…”

Section: Resultsmentioning

confidence: 99%

Use of Lactic Acid Bacteria to Reduce Methane Production in Ruminants, a Critical Review

Doyle

Mbandlwa

Kelly³

et al. 2019

Front. Microbiol.

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Enteric fermentation in ruminants is the single largest anthropogenic source of agricultural methane and has a significant role in global warming. Consequently, innovative solutions to reduce methane emissions from livestock farming are required to ensure future sustainable food production. One possible approach is the use of lactic acid bacteria (LAB), Gram positive bacteria that produce lactic acid as a major end product of carbohydrate fermentation. LAB are natural inhabitants of the intestinal tract of mammals and are among the most important groups of microorganisms used in food fermentations. LAB can be readily isolated from ruminant animals and are currently used on-farm as direct-fed microbials (DFMs) and as silage inoculants. While it has been proposed that LAB can be used to reduce methane production in ruminant livestock, so far research has been limited, and convincing animal data to support the concept are lacking. This review has critically evaluated the current literature and provided a comprehensive analysis and summary of the potential use and mechanisms of LAB as a methane mitigation strategy. It is clear that although there are some promising results, more research is needed to identify whether the use of LAB can be an effective methane mitigation option for ruminant livestock.

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Section: Resultsmentioning

confidence: 99%

Use of Lactic Acid Bacteria to Reduce Methane Production in Ruminants, a Critical Review

Doyle

Mbandlwa

Kelly³

et al. 2019

Front. Microbiol.

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show abstract

“…In addition, a protease‐resistant antimicrobial compound (PRA‐1) is produced by Lactobacillus . Lactobacillus plantarum TUA1490L is capable of inhibiting or reducing methane production (Takahashi et al ; Asa et al ). However, there is little or no information on the use of Lactobacillus farciminis on ruminant fermentation activity, especially their impacts on production of biogases.…”

Section: Introductionmentioning

confidence: 99%

Role of dose‐dependent Lactobacillus farciminis on ruminal microflora biogases and fermentation activities of three silage‐based rations

et al. 2019

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Aims The influence of Lactobacillus farciminis on ruminal fermentation characteristics was elucidated in this study. Methods and Results Ruminal fermentation was conducted using maize silage ration (R) and concentrate (C) as 75R:25C, 50R:50C and 25R:75C, supplemented with lactic acid bacteria (LB) at 0, 20 and 30 mg g−1 dry matter substrate and their interaction (1st experiment). The same LB product was used at 0, 20, 40 and 60 mg g−1 dry matter of the mixture (1 : 1) of oat straw and concentrate for 48 h of incubation (2nd experiment). At 24 and 48 h of incubation, LB0 produced the highest biogas and LB20 produced the lowest, whereas at 48 h of incubation LB40 produced the lowest. In ration x LB, LB40 resulted in the highest biogas production, while LB0 had the lowest (P < 0·001) at 8, 10 and 12 h of incubation. Inclusions of LB0, 20, 40 and 60 mg g−1 dry matter resulted in a linear increase (P < 0·003) in the asymptotic biogas production and fermentation parameters in a dose‐dependent manner, except in pH which decreased (P = 0·029). Conclusions The use of L. farciminis in diet with high level of concentrate without any adverse effect on the pH of rumen fluid to the point of acidosis. Furthermore, in high forage diet, the use of L. farciminis would help to improve the ruminal fermentation digestibility and mitigate ruminal biogas production. Significance and Impact of the Study Using Lactobacillus as a feed additive can improve ruminal fermentation activities by maintaining the stability of pH in the rumen and improving the feed utilization through manipulation of the microbial ecosystem.

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“…Nisin obtained from Lactobacillus lactis ssp. Recently, highly specifi c antibacterial activity of PRA-1 produced by Lactobacillus plantarum TUA1490L against methanogens was reported by Asa et al ( 2010 ). It is nearly as potent a methane inhibitor as monensin, and it was just as effective in decreasing the acetate to propionate ratio and has been reported that 36 % methanogenesis was reduced by the use of nisin (Callaway et al 1997 ).…”

Section: Use Of Bacteriocinsmentioning

confidence: 99%

Climate Change Impact on Livestock: Adaptation and Mitigation

Sejian¹,

Gaughan²,

Baumgard³

et al. 2015

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Effects of Protease-resistant Antimicrobial Substances Produced by Lactic Acid Bacteria on Rumen Methanogenesis

Cited by 19 publications

References 38 publications

Use of Lactic Acid Bacteria to Reduce Methane Production in Ruminants, a Critical Review

Use of Lactic Acid Bacteria to Reduce Methane Production in Ruminants, a Critical Review

Role of dose‐dependent Lactobacillus farciminis on ruminal microflora biogases and fermentation activities of three silage‐based rations

Climate Change Impact on Livestock: Adaptation and Mitigation

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