Effects of protein kinase inhibitors on pig oocyte maturation in vitro

Jung, Thomas; Lee, C.; Moor, R. M.

doi:10.1051/rnd:19920506

Cited by 14 publications

(10 citation statements)

References 27 publications

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“…In porcine oocytes, PKC was observed to participate in the regulation of the resumption of meiotic maturation (Jung et al, 1992;Coskun and Lin, 1995;Kim and Menino, 1995;Su et al, 1999;Shimada et al, 2001). Fan et al (2002b) demonstrated a change in the localization of PKC -α, -β I , -γ in maturing porcine oocytes and after their activation.…”

Section: Which Signalling Cascades Remained Silent In No-induced Oocymentioning

confidence: 99%

The role of nitric oxide in parthenogenetic activation of pig oocytes: A review

Petr¹,

Eva²,

Tůmová³

et al. 2007

CZECH J ANIM SCI

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Parthenogenetic activation of mammalian oocytes with artificial stimuli is commonly applied in various reproductive biotechniques, e.g. cloning using nuclear transfer. For this reason, many studies focus on oocyte activation in vitro. Recently we have described the activation of pig oocytes using nitric oxide. This activating stimulus is very specific in many aspects. However, it does not provide an adequate stimulus for parthenogenetic development. It was shown that nitric oxide stimulated some signalling pathways which are inactive in conventional treatments for parthenogenetic activation, e.g. the cGMP-dependent signalling cascade. On the other hand, nitric oxide does not stimulate certain signalling pathways involved in oocyte activation after calcium ionophore, e.g. the PKC signalling cascade. The aim of this review is to characterize the complex processes induced in oocytes after treatment with nitric oxide. Perspectives for further research and the application of nitric oxide for parthenogenetic activation of oocytes are outlined.

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Section: Which Signalling Cascades Remained Silent In No-induced Oocymentioning

confidence: 99%

The role of nitric oxide in parthenogenetic activation of pig oocytes: A review

Petr¹,

Eva²,

Tůmová³

et al. 2007

CZECH J ANIM SCI

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“…(Jung et al, 1992 (Besterman and Schultz, 1990 (Besterman and Schultz, 1990). Mouse oocyte matu¬ ration is inhibited by genistein in a dose-dependent manner (ED50: 20 pg ml"1).…”

Section: Introductionmentioning

confidence: 99%

Effects of the protein phosphorylation inhibitor genistein on maturation of pig oocytes in vitro

Jung¹,

Fulka²,

Lee³

et al. 1993

Reproduction

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“…In other experiments, however, genistein failed to prevent EGF [33]-and IGF-I [38]induced cumulus expansion and oocyte maturation, suggesting that TK does not mediate growth factor effects on meiosis. Protein kinase C is probably not involved in this process [17,37].…”

Section: Introductionmentioning

confidence: 99%

Evidence that growth factors IGF-I, IGF-II and EGF can stimulate nuclear maturation of porcine oocytes via intracellular protein kinase A

et al. 2000

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-The aim of our in vitro experiments was to study the role of growth factors and protein kinase A (PKA)-dependent intracellular mechanisms in the control of nuclear maturation of porcine oocytes. Oocytes were cultured with or without growth factors (IGF-I, IGF-II, EGF; 10 ng . mL -1 medium) and inhibitors of PKA (Rp-cAMPS or KT5720; 100 ng . mL -1 ). Stages of meiosis were determined from the structure of chromosomes after staining with Giemza. Intracellular levels of PKA were evaluated immunocytochemically using primary antisera against the PKA regulatory and catalytic subunits and by Western immunoblotting using primary antiserum against the PKA catalytic subunit. It was found that after 24 h culture the majority of oocytes had resumed nuclear maturation (they were at a stage of meiosis after diplotene) and that after 48 h culture the majority of cells had completed maturation (they had reached metaphase II of meiosis). Addition of IGF-I, IGF-II or EGF, or a combination of IGF-I and EGF, significantly increased the proportion of oocytes which resumed and completed meiosis. Immunocytochemistry demonstrated a significant increase in the proportion of cells containing catalytic and, in some cases, the regulatory subunits of PKA after addition of IGF-I, IGF-II and EGF. Immunoblotting showed the presence of 2 forms of the PKA catalytic subunit within the oocytes (MW approximately 52 and 40 kD). EGF, but not IGF-I or IGF-II, increased the content of both isoforms. Inhibitors of PKA, when given alone, did not substantially influence the proportion of oocytes which resumed or completed meiosis. However, Rp-cAMPS and KT5720 both prevented the stimulatory effects of IGF-I, IGF-II and EGF on the resumption and completion of oocyte maturation. The present observations suggest (1) that IGF-I, IGF-II and EGF are potent stimulators of both resumption and completion of porcine oocyte nuclear maturation, (2) that PKA is present in oocytes, and (3) that PKA-dependent intracellular mechanisms can mediate the action of growth factors on porcine oocytes.insulin-like growth factor / epidermal growth factor / protein kinase A / meiosis Reprod. Nutr. Dev. 40 (2000)

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Effects of protein kinase inhibitors on pig oocyte maturation in vitro

Abstract: porc / maturation des ovocytes / protéine kinase C / protéine kinase AMP! dépendante / calmoduline

Cited by 14 publications

References 27 publications

The role of nitric oxide in parthenogenetic activation of pig oocytes: A review

The role of nitric oxide in parthenogenetic activation of pig oocytes: A review

Effects of the protein phosphorylation inhibitor genistein on maturation of pig oocytes in vitro

Evidence that growth factors IGF-I, IGF-II and EGF can stimulate nuclear maturation of porcine oocytes via intracellular protein kinase A

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