Effects of protein kinase modulators on transferrin receptor expression in human leukaemic HL‐60 cells

Lok, Chun Nam; Chan, Kwok‐Ping; Loh, Tatt‐Tuck

doi:10.1016/0014-5793(95)00442-c

Cited by 5 publications

(3 citation statements)

References 23 publications

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“…From these observations, it seems that protein kinase C and cAMP signaling are involved in differentiation-associated downregulation of TfR gene expression. Recent experiments using specific inhibitors for protein kinase C and A, respectively, showed that activation of protein kinase C or A are required during the downregulation of TfR mRNA in the differentiating HL-60 cells [156]. As discussed before, the IRP binding activity can be modulated by protein phosphorylation, oxidative stress and nitric oxide, allowing a more precise control of the TfR expression at the level of mRNA stability in response to a variety of hormonal and inflammatory stimuli.…”

Section: Cellular Signalings Involved In the Tfr Expressionmentioning

confidence: 99%

Regulation of Transferrin Function and Expression: Review and Update

Lok¹,

Loh²

1998

Biol Signals Recept

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The cellular iron uptake is a precisely controlled process to fulfill the iron demand for the synthesis and functions of a variety of iron-containing proteins, and one of the main molecules involved is the transferrin receptor (TfR), which mediates the uptake process via the transferrin cycle. The TfR expression is tightly regulated by factors such as intracellular iron level, cell proliferation or erythropoiesis at levels of receptor recycling, transcriptional or posttranscriptional control. The iron-regulatory protein/iron-responsive element system has been widely used to explain changes in receptor expression during iron loading or depletion, oxidative stress and nitric oxide stimulation. On the other hand, transcriptional control of TfR expression appears to be more important in erythroid differentiation and general cell proliferation. There is also an increasing awareness of the clinical application and experimental therapeutics based on the TfR functioning and expression. In this review, we attempt to provide a concise account of the studies of TfR structure and function as well as those areas that have not been reviewed in depth, in particular, tissue-specific regulation of TfR, the molecular mechanisms of TfR expression, and the use of TfR as diagnostic and therapeutic tools. The regulation of TfR expression in various tissues is related to its specific cellular iron requirements. Hemoglobin-synthesizing cells exhibit distinct features of iron metabolism and TfR expression as compared to most non-erythroid cells which synthesize a much lower amount of heme. For most non-erythroid cells, iron can regulate the TfR expression in a reciprocal manner through modulating the stability of the receptor mRNA whereas in hemoglobin-synthesizing cells, the TfR expression is independent of the cellular iron loading. In spite of a wide heterogeneity in the way receptor redistribution is in response to various stimuli, regulation of the constitutive expression of TfR is one of the ways of regulating the cellular iron uptake. This expression operates on both transcriptional and posttranscriptional levels. In general, factors related to cell growth and differentiation operate on the gene transcription level, whereas iron regulates the fate of the mature mRNA.

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Section: Cellular Signalings Involved In the Tfr Expressionmentioning

confidence: 99%

Regulation of Transferrin Function and Expression: Review and Update

Lok¹,

Loh²

1998

Biol Signals Recept

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“…Second, as mentioned above, the biological effects of estrogens have also been linked to cell membrane receptor-coupled signal transduction systems involving conventional second messengers (Nabekura et al, 1986;Zakon, 1998;Falkenstein et al, 2000;Cato et al, 2002). Some of these second messenger systems, such as protein kinase A and mitogen-activated signal transduction pathways, were also reportedly responsible for the regulation of transferrin receptor expression (Ouyang et al, 1993;Lok et al, 1995).…”

Section: Sult1e1 Transfection and Signal Transductionmentioning

confidence: 99%

Transfection of Human Prostate Cancer CA-HPV-10 Cells with Cytosolic Sulfotransferase SULT1E1 Affects Estrogen Signaling and Gene Transcription

Kapoor

Sheng²

2007

Drug Metab Dispos

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ABSTRACT:Human cytosolic sulfotransferase SULT1E1 catalyzes the sulfation of estrogens and estrogenic drugs in human reproductive tissues. Logically, this estrogen-preferring sulfotransferase isoform could play a regulatory role in estrogen signaling activities in human reproductive cells, including the prostate cells. This hypothesis was tested using DNA microarray and real-time reverse transcription-polymerase chain reaction methods in the present work. Potential changes in the transcriptional expression of selected signal transduction-related genes in human prostate cancer CA-HPV-10 cell line after SULT1E1 transfection were examined by DNA microarray methods. Notable changes were observed in the mRNA expression levels of TFRC, a cell membrane transferrin receptor gene, and TMEPAI, a gene encoding a steroid-dependent mRNA product. Expression of TFRC was down-regulated, whereas expression of TMEPAI was up-regulated by SULT1E1 transfection in CA-HPV-10 cells. Data from the current studies also showed that the estrogen-induced estrogen response element activation in CA-HPV-10 cells was repressed after the cells were transfected with SULT1E1. These results indicate that SULT1E1 may function as a transcriptional mediator in human prostate cancer CA-HPV-10 cells.

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“…H-89 exhibits a potent and selective inhibitory action on PKA §. Many studies have used H-89 to assess the function of PKA in cells [2][3][4][5].…”

Section: H-89 N-[2-(p-bromocinnamylamino)ethyl]-5-isoquinolinementioning

confidence: 99%

Inhibition of 12-O-tetradecanoylphorbol-13-acetate induction of c-fos mRNA by the protein kinase A inhibitor N-[2-(p-bromocinnamylamino)ethyl]-5-isoquinoline sulfonamide

Huang

Lin

Lee

1999

Biochemical Pharmacology

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Effects of protein kinase modulators on transferrin receptor expression in human leukaemic HL‐60 cells

Cited by 5 publications

References 23 publications

Regulation of Transferrin Function and Expression: Review and Update

Regulation of Transferrin Function and Expression: Review and Update

Transfection of Human Prostate Cancer CA-HPV-10 Cells with Cytosolic Sulfotransferase SULT1E1 Affects Estrogen Signaling and Gene Transcription

Inhibition of 12-O-tetradecanoylphorbol-13-acetate induction of c-fos mRNA by the protein kinase A inhibitor N-[2-(p-bromocinnamylamino)ethyl]-5-isoquinoline sulfonamide

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