Effects of Pseudomonas aeruginosa on Immune Functions

Campa, Mario; Marelli, P; Lupetti, Antonella

doi:10.1007/978-1-4615-3036-7_11

Cited by 8 publications

(6 citation statements)

References 100 publications

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“…The Gram negative bacterium Pseudomonas aeruginosa , an opportunistic pathogen (Whiteley et al 2001; Campa et al 2012; Oberhardt et al 2008), is one of the most prevalent colonizer of medical devices in immuno-compromised patients (Kiehn and Armstrong 1990; Roy-Burman et al 2001; Falkinham et al 2015; Waldvogel and Bisno 2000). The objectives of the study were to evaluate the proneness of the surfaces of two widely used biomaterials toward biofilm formation, and identify the roles of implant surface and conditioning layer in modulating the biofilm architecture.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the role of substrate and albumin on Pseudomonas aeruginosa biofilm morphology through FESEM and FTIR studies on polymeric biomaterials

et al. 2017

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Bacterial biofilms pose the greatest challenge to implant surgeries leading to device-related infections and implant failure. Our present study aims at monitoring the variation in the biofilm architecture of a clinically isolated strain and ATCC 27853 strain of Pseudomonas aeruginosa on two polymeric biomaterials, used in implants. The perspective of our study is to recognize the potential of these two biomaterials to create biofilm infections and develop the understanding regarding their limitations of use and handle patients with this deeper insight. The final goal, however, is an accurate interpretation of substrate-microbe interactions in the two biomaterials, which will provide us the knowledge of possible surface modifications to develop of an efficacious anti-biofilm therapy for deterring implant infections. The reference strain ATCC 27853 and a clinical isolate of P. aeruginosa collected from urinary catheters of patients suffering from urinary tract infections, have been used as microbes while clinical grades of polypropylene and high density polyethylene, have been used as ‘substrates’ for biofilm growth. The variation in the nature of the ‘substrate’ and ‘conditioning layer’ of BSA have been found to affect the biofilm architecture as well as the physiology of the biofilm-forming bacteria, accompanied by an alteration in the nature and volume of EPS (extracellular polysaccharide) matrices.Graphical Abstract Electronic supplementary materialThe online version of this article (doi:10.1007/s40204-017-0061-2) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

Evaluation of the role of substrate and albumin on Pseudomonas aeruginosa biofilm morphology through FESEM and FTIR studies on polymeric biomaterials

et al. 2017

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“…Pseudomonas aeruginosa is a versatile Gram-negative pathogenic bacterium, that can cause various infections in human ( de Bentzmann and Plésiat, 2011 ; Campa et al, 2012 ). During infection, P. aeruginosa produces multiple virulence factors to facilitate colonization ( Sadikot et al, 2005 ; Kipnis et al, 2006 ; Hauser, 2009 ; Liu et al, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

PA3297 Counteracts Antimicrobial Effects of Azithromycin in Pseudomonas aeruginosa

et al. 2016

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Pseudomonas aeruginosa causes acute and chronic infections in human. Its increasing resistance to antibiotics requires alternative treatments that are more effective than available strategies. Among the alternatives is the unconventional usage of conventional antibiotics, of which the macrolide antibiotic azithromycin (AZM) provides a paradigmatic example. AZM therapy is associated with a small but consistent improvement in respiratory function of cystic fibrosis patients suffering from chronic P. aeruginosa infection. Besides immunomodulating activities, AZM represses bacterial genes involved in virulence, quorum sensing, biofilm formation, and motility, all of which are due to stalling of ribosome and depletion of cellular tRNA pool. However, how P. aeruginosa responds to and counteracts the effects of AZM remain elusive. Here, we found that deficiency of PA3297, a gene encoding a DEAH-box helicase, intensified AZM-mediated bacterial killing, suppression of pyocyanin production and swarming motility, and hypersusceptibility to hydrogen peroxide. We demonstrated that expression of PA3297 is induced by the interaction between AZM and ribosome. Importantly, mutation of PA3297 resulted in elevated levels of unprocessed 23S-5S rRNA in the presence of AZM, which might lead to increased susceptibility to AZM-mediated effects. Our results revealed one of the bacterial responses in counteracting the detrimental effects of AZM.

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“…It is frequently involved in ear and eye infections (Fleiszig et al, 1994) and could be considered as the main pathogen responsible for chronic pneumonia in patients developing cystic fibrosis (Doring, 1993; Romling et al, 1994). Among the multiple factors recognized to be involved in the virulence of P. aerugiosa (for details see Campa et al, 1993), nutritional factors should play a primary role for the development of bacteria in vivo. A particularly crucial factor is bacterial iron nutrition, this element being absolutely necessary for most micro-organisms, especially for aerobic bacteria such as the pseudo-monads.…”

Section: Introductionmentioning

confidence: 99%

Use of Siderophores to Type Pseudomonads: The Three Pseudomonas Aeruginosa Pyoverdine Systems

et al. 1997

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Eighty-eight Pseudomonas aenrginosa isolates, most of them from the Collection of Bacterial Strains of the lnstitut Pasteur, Paris, were analysed for their pyoverdine-mediated iron incorporation system by different methods, including pyoverdine isoelectrofocusing analysis, pyoverdine-mediated growth stimulation, immunoblot detection of (ferri)pyoverdine outer-membrane receptor and pyoverdine-facilitated iron uptake. The same grouping of the strains was reached by each of these methods, resulting in the classification of the P. aenrginosa isolates, even those which were devoid of pyoverdine production, into three different siderophore types. Forty-two percent of the strains were identified with the --strain P. aeruginosa ATCC 15692 (group I), 42% were identical with the second type-strain P. aenrginosa ATCC 27853 (group II) and 16% reacted identically with the clinical isolate P. aenrginosa Pa6, whose pyoverdine was recognized in this study to be identical in structure to the pyoverdine produced by a natural isolate, P. aeruginosa strain R. No new pyoverdine species was detected among these strains.

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Effects of Pseudomonas aeruginosa on Immune Functions

Cited by 8 publications

References 100 publications

Evaluation of the role of substrate and albumin on Pseudomonas aeruginosa biofilm morphology through FESEM and FTIR studies on polymeric biomaterials

Evaluation of the role of substrate and albumin on Pseudomonas aeruginosa biofilm morphology through FESEM and FTIR studies on polymeric biomaterials

PA3297 Counteracts Antimicrobial Effects of Azithromycin in Pseudomonas aeruginosa

Use of Siderophores to Type Pseudomonads: The Three Pseudomonas Aeruginosa Pyoverdine Systems

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