Evaluation of the role of substrate and albumin on Pseudomonas aeruginosa biofilm morphology through FESEM and FTIR studies on polymeric biomaterials

Sinha, Suparna; Chatterjee, Susmita; Maiti, Prabal K.; Tarafdar, Sujata; Moulik, Satya P.

doi:10.1007/s40204-017-0061-2

Cited by 18 publications

(8 citation statements)

References 84 publications

(86 reference statements)

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“…The protocol for biofilm formation however remanins the same throughout our experiments for all substrates and all conditioning layers for all strains of the microbes. Hence the experimental results can be considered to be quite robust as also reported previously in the work of Dutta Sinha et al The calculation of different statistical features were done on each set of data separately (also by resampling each image) and their variability has also been reported in the final results.…”

Section: Description Of the Statistical Features Of Biofilm Imagessupporting

confidence: 62%

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Monitoring of Wild Pseudomonas Biofilm Strain Conditions Using Statistical Characterization of Scanning Electron Microscopy Images

Sinha

Das

Tarafdar

et al. 2017

Ind. Eng. Chem. Res.

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Abstract:The present paper proposes a novel method of quantification of the variation in biofilm architecture, in correlation with the alteration of growth conditions that include, variations of substrate and conditioning layer. The polymeric biomaterial serving as substrates are widely used in implants and indwelling medical devices, while the plasma proteins serve as the conditioning layer. The present method uses descriptive statistics of FESEM images of biofilms obtained during a variety of growth conditions. We aim to explore here the texture and fractal analysis techniques, to identify the most discriminatory features which are capable of predicting the difference in biofilm growth conditions. We initially extract some statistical features of biofilm images on bare polymer surfaces, followed by those on the same substrates adsorbed with two different types of plasma proteins, viz. Bovine serum albumin (BSA) and Fibronectin (FN), for two different adsorption times. The present analysis has the potential to act as a futuristic technology for developing a computerized monitoring system in hospitals with automated image analysis and feature extraction, which may be used to predict the growth profile of an emerging biofilm on surgical implants or similar medical applications.

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Section: Description Of the Statistical Features Of Biofilm Imagessupporting

confidence: 62%

“…The strain was previously reported to be a strong biofilm former by 96 well microtiter plate assay. The goal was to prove experimentally that the pathogenic strain responsible for urinary infections (forming biofilm on silicon rubber) is very much liable to affect orthopedic implants.…”

Section: Methodsmentioning

confidence: 99%

Monitoring of Wild Pseudomonas Biofilm Strain Conditions Using Statistical Characterization of Scanning Electron Microscopy Images

Sinha

Das

Tarafdar

et al. 2017

Ind. Eng. Chem. Res.

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“…Tris buffer was obtained from Sigma-Aldrich, while BSA was from MP Biomedical Ltd. The polymers chips were initially cleaned in an ultrasonic cleaner, rinsed with water, blow-dried, and preserved in a vacuum desiccator, ready for carrying out adsorption . BSA was mixed in the proportion 0.5 mg/mL with buffer solutions of pH 7.4 and left for 1 week with intermittent mixing to dissolve the BSA completely.…”

Section: Bovine Serum Albumin-coated Polymer Substratesmentioning

confidence: 99%

“…The protocol for biofilm growth was followed with the four sets of polymer chips with BSA adsorbed on them for 24 h, and three samples of each type were placed in separate wells of a 24 well tissue culture plate with identical growth conditions. Biofilms were simultaneously grown on three samples of each type of bare polymer (without BSA), following the same protocol.…”

Section: Bacterial Biofilms On Bsa-coated Polymer Substratesmentioning

confidence: 99%

Decisive Role of Polymer–Bovine Serum Albumin Interactions in Biofilm Substrates on “Philicity” and Extracellular Polymeric Substances Composition

et al. 2022

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Formation of extracellular polymeric substances (EPS) is a crucial step for bacterial biofilm growth. The dependence of EPS composition on growth substrate and conditioning of the latter is thus of primary importance. We present results of studies on the growth of biofilms of two different strains each, of the Gram-negative bacteria Escherichia coli and Klebsiella pneumoniae, on four polymers used commonly in indwelling medical devices polyethene, polypropylene, polycarbonate, and polytetrafluoroethyleneimmersed in bovine serum albumin (BSA) for 24 h. The polymer substrates are studied before and after immersing in BSA for 9 and 24 h, using contact angle measurement (CAM) and field emission scanning electron microscopy (FE-SEM) to extract, respectively, the "philicity" φ (defined as −cos θ, where θ is the contact angle of the liquid on the solid at a particular temperature and ambient pressure) and spatial Hirsch parameter H (defined from the relation F(r) ∼ r 2H , where F(r) is the mean squared density fluctuation at the sample surface). H = 0.5, <0.5, or >0.5 signifies no correlation, anticorrelation, and correlation, respectively. The substrates are seen to transform from large hydrophobicity to near amphiphilicity with the formation of a BSA conditioning surface layer, and the H-values distinguish the length scales of 100, 500, and 2000 nm, with the anticorrelation increasing with length scale. Biofilms of E. coli did not grow on bare PTFE and HDPE substrates. Biofilms grown on BSA-covered surfaces are studied with CAM, FE-SEM, Fourier transform infrared (FTIR), and surface-enhanced Raman spectroscopy (SERS). Both spectra and φ-values were independent of bacterial species but dependent on the polymer, while H-values show some bacterial variation. Thus, EPS composition and wetting properties of the corresponding bacterial biofilms seem to be decided by the interaction of the conditioning BSA layer with the specific polymer substrate.

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“…In phase I the bacteria get attached to the surface reversibly over the first 1–2 hr, which is mediated through weak and mostly dispersive forces. Phase II begins approximately 2–3 hr later and is characterized by irreversible adhesion between the bacteria and the surface which is mediated through adhesive molecules secreted by the bacteria [3]. In phases III and IV, biofilm matures through the processes of chemical cross talk, production of Extracellular Poly-saccharides (EPS), and micro-colony formation.…”

Section: Introductionmentioning

confidence: 99%

Periodicities in the roughness and biofilm growth on glass substrate with etching time: Hydrofluoric acid etchant

et al. 2019

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Adherence of the microorganism to submerged solid surfaces leads to biofilm formation. Biofilm formation modifies the surfaces in favor of bacteria facilitating the survival of the bacteria under different stressed conditions. On the other hand, the formation of biofilm has a direct adverse economic impact in various industries and more importantly in medical practices. This adherence is the reason for the failure of many indwelling medical devices. Surface biofilm adhesion is the key to biofilm growth and stability. Hence this adhesion needs to be substantially lowered to inhibit biofilm stability. Both chemical and physical properties of the surface influence biofilm formation and modulating these properties can control this formation. In this study, we have investigated the effect of Hydrofluoric acid (HF), at a specific concentration as an etchant, on the surface morphology of substrates and the growth of biofilms of Pseudomonas aeruginosa . and Staphylococcus aureus . We find that the bacterial counts on the etched surfaces undergo a periodic increase and decrease. This, on one hand, shows the close correlation between the biofilm growth and the particular roughness scale, and on the other hand, explains the existing contradictory results regarding the effects of etching on substrate roughness and biofilm growth. We propose a simple model of a sequence of hole formation, hole expansion and etching away of the hole walls to form a new, comparatively smooth surface, coupled with the preferential accumulation of bacteria at the hole edges, to explain these periodicities.

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Evaluation of the role of substrate and albumin on Pseudomonas aeruginosa biofilm morphology through FESEM and FTIR studies on polymeric biomaterials

Cited by 18 publications

References 84 publications

Monitoring of Wild Pseudomonas Biofilm Strain Conditions Using Statistical Characterization of Scanning Electron Microscopy Images

Monitoring of Wild Pseudomonas Biofilm Strain Conditions Using Statistical Characterization of Scanning Electron Microscopy Images

Decisive Role of Polymer–Bovine Serum Albumin Interactions in Biofilm Substrates on “Philicity” and Extracellular Polymeric Substances Composition

Periodicities in the roughness and biofilm growth on glass substrate with etching time: Hydrofluoric acid etchant

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