Effects of pulsed electromagnetic fields on lipid peroxidation and antioxidant levels in blood and liver of diabetic rats

Abstract

Objective: The present study investigated the protective effects of a pulsed electromagnetic field (PEMF) in a rat model of diabetes by analyzing oxidative/nitrosative stress parameters. Methods: Rats were randomly divided into four groups of eight each: a control group, a sham PEMF group, a diabetes group, and a diabetes+PEMF group. Diabetes was induced in the sham PEMF, diabetes, and diabetes+PEMF groups by treatment with 50 mg/ kg streptozotocin (STZ). Rats in the sham PEMF group were treated identically, without running the instrument. Following the development of diabetes, rats in the diabetes+PEMF group were treated with PEMF for 60 min/day for 4 weeks. Results: Levels of oxidants, such as malondialdehyde (MDA), nitric oxide (NO), and myeloperoxidase (MPO), and antioxidants, such as superoxide dismutase (SOD), glutathione (GSH), and catalase (CAT), were measured in blood and liver tissue samples. MPO, MDA, and NO levels were significantly higher, and SOD levels significantly lower, in the sham PEMF and diabetes groups than in the control group (p<0.05 each), whereas the levels of all these four (i.e.: MPO, MDA, NO, and SOD) in the diabetes+PEMF group were close to those in the control group. GSH levels were significantly lower in the sham PEMF, diabetes, and diabetes+PEMF groups than in the control group (p<0.05 each), whereas CAT levels were similar in all the four groups. Conclusion: Results indicate that PEMF affects MDA, NO, MPO, SOD, and GSH levels and regulates diabetes-associated damage by reducing oxidative stress and increasing the levels of antioxidants. PEMF may be a non-invasive treatment option for diabetes and associated complications.

In Vitro Antioxidant Activities and the Therapeutic Potential of Some Newly Synthesized Chalcones Against 4-Acetaminophenol Induced Hepatotoxicity in Rats

“…According to the literature, serum MDA levels were increased whereas CAT and SOD levels were decreased in hepatic damage. 27 In the present study, significant reduction in serum MDA and elevation in CAT and SOD levels followed by administration of the test compounds reflected their antioxidant activity that was comparable with standard hepatoprotective drug, Silymarin. In vitro antioxidant potential of test chalcones determined by DPPH free radical scavenging method, showed significant (p ≤ 0.05) antioxidant activities.…”

Effects of Emulsion Formulations of Oleuropein Isolated from Ethanol Extract of Olive Leaf in Diabetic Rats

“…Also, it is believed that reactive species in diabetes may increase due to a drastic change in SOD activities and GSH levels (Sugumar et al 2016). SOD and GSH are a crucial part of the antioxidant defense mechanism (Gozen et al 2017). In the present study, the SOD and GSH values of the diabetic rats are much lower when compared to those of the untreated rats.…”

“…It is used as an indicator of oxidative stress in tissues and cells. TBARS is a standard marker for screening and monitoring lipid peroxidation (Gozen et al 2017, Jemai et al 2009). In the current study, after the injection of STZ, TBARS levels of the diabetic rats were significantly increased.…”

“…Approximately 4 to 6 hours after the injection, 5% doses of dextrose solution were given to the rats for 24 hours due to lethal hypoglycemia resulting from massive beta cell destruction. Seventy two hours following the STZ injection, the animals with non-fasting blood glucose levels (BGLs) above 300 mg/dL and symptoms of polyuria and polydipsia were considered to be diabetic (Chattopadhyay 1999, Gozen et al 2017.…”

The Effects of Electromagnetic Fields on Human Health: Recent Advances and Future

“…PEMFs have been found to accelerate wound healing and improve the mechanical properties of soft tissue in diabetic rats [89,90]. Furthermore, it has been demonstrated that PEMFs can restore the normal antioxidative stress function in diabetic rats by reducing oxidant levels and increasing antioxidant levels [91]. Interestingly, it has also been reported that 1.5 mT PEMF at 1 Hz, 10 Hz, 20 Hz and 40 Hz can relieve other symptoms related to diabetes, such as hyperalgesia and allodynia [92].…”