Effects of purified human native granulocyte colony‐stimulating factor (G‐CSF) on proliferation of blast progenitors in acute myeloblastic leukemia

Nara, Nobuo; Murohashi, Ikuo; Suzuki, Toshiya; Yamashita, Yoko; Maruyama, Yasuo; Aoki, Nobuo; Tanikawa, S; Onozawa, Y

doi:10.1002/ajh.2830270203

Cited by 12 publications

References 20 publications

(26 reference statements)

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Flow cytometric detection of recombinant human granulocyte‐colony stimulating factor binding to leukemic cells

Tatsumi

Yamane

Izumi

et al. 1993

Clinical Laboratory Analysis

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To examine binding of recombinant human granulocyte-colony stimulating factor to myeloid cells, the factor was labeled with fluorescein isothiocyanate, and incubated with blood specimens, which were then analyzed by flow cytometry. Neutrophils demonstrated an increased fluorescence, while lymphocytes were negative. These cell fractions were used as controls for cytometric binding assays of leukemic cells. Six patients with lymphocytic leukemia were negative in this assay. Ten of 15 patients with myelocytic leukemia were positive. All patients (n = 5) in myeloblastic crisis of chronic myelogenous leukemia were also positive. The flow cytometry results correlated well with the results of colony formation in response to granulocyte-colony stimulating factor. The results indicate that our method is useful in predicting the susceptibility of leukemic cells to recombinant growth factors.

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Flow cytometric detection of recombinant human granulocyte‐colony stimulating factor binding to leukemic cells

Tatsumi

Yamane

Izumi

et al. 1993

Clinical Laboratory Analysis

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Response of leukemic cells to the sequential combination of gm‐csf and g‐csf

Inoue

Murate

Hotta

et al. 1990

The International Journal of Cell Cloning

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We designed two experiments using delayed stimulation by granulocyte/macrophage colony-stimulating factor (GM-CSF) or granulocyte colony-stimulating factor (G-CSF) with colony-type analysis in order to elucidate the characteristics of factor-dependent proliferation and differentiation of leukemic progenitor cells. Eight patients with acute myelogenous leukemia showing non-autonomous colony growth were selected. Three of the six cases showed that delayed addition of G-CSF to agar culture initiated by GM-CSF had no influence on the type and number of colonies; whereas, delayed addition of GM-CSF to G-CSF resulted in additional GM colonies. These findings were the same for normal bone marrow progenitors. In two cases, granulocytic colonies markedly increased with delayed addition of G-CSF to GM-CSF. Synergism occurred in one case where GM-CSF was used first. In the next experiment, regardless of whether CSF was used during the 48 hour preincubation, subsequent colony types were affected by the CSF used in agar culture. But colony numbers increased 1.5 fold in two of six cases by preincubation with GM-CSF, and 1.2 to 1.7 fold in all normal cases by preincubation with G-CSF. These results suggest that although there is a great heterogeneity in the response to CSF, some leukemic progenitors act like normal progenitors.

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Zytokine in der klinischen Anwendung

Meyer

Wilms

1988

Der Internist

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Effects of purified human native granulocyte colony‐stimulating factor (G‐CSF) on proliferation of blast progenitors in acute myeloblastic leukemia

Cited by 12 publications

References 20 publications

Flow cytometric detection of recombinant human granulocyte‐colony stimulating factor binding to leukemic cells

Flow cytometric detection of recombinant human granulocyte‐colony stimulating factor binding to leukemic cells

Response of leukemic cells to the sequential combination of gm‐csf and g‐csf

Zytokine in der klinischen Anwendung

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