Effects of radiofrequency field exposure on proteotoxic-induced and heat-induced HSF1 response in live cells using the bioluminescence resonance energy transfer technique

Poque, Emmanuelle; Ruigrok, Hermanus; Arnaud‐Cormos, Delia; Habauzit, Denis; Chappe, Yann; Martin, Catherine; Gannes, F. Poulletier de; Hurtier, Annabelle; Garenne, André; Lagroye, I.; Dréan, Yves Le; Lévêque, Philippe; Percherancier, Yann

doi:10.1007/s12192-020-01172-3

Cited by 5 publications

(11 citation statements)

References 49 publications

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“…The approaches previously used to study the cellular and molecular effects of low-level RF exposure have produced at best either large-scale data (such as “omics”) [ 6 , 7 , 8 ] or data acquired in real time and live cells, but focusing only on one cellular or molecular mechanism (e.g., the BRET approach that we developed recently [ 9 , 10 , 11 ]). Unfortunately, large-scale data are obtained from cell extracts following RF exposure, using, in most cases, stringent preparative steps.…”

Section: Introductionmentioning

confidence: 99%

Label-Free Study of the Global Cell Behavior during Exposure to Environmental Radiofrequency Fields in the Presence or Absence of Pro-Apoptotic or Pro-Autophagic Treatments

Joushomme

Garenne

Dufossée

et al. 2022

IJMS

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It remains controversial whether exposure to environmental radiofrequency signals (RF) impacts cell status or response to cellular stress such as apoptosis or autophagy. We used two label-free techniques, cellular impedancemetry and Digital Holographic Microscopy (DHM), to assess the overall cellular response during RF exposure alone, or during co-exposure to RF and chemical treatments known to induce either apoptosis or autophagy. Two human cell lines (SH-SY5Y and HCT116) and two cultures of primary rat cortex cells (astrocytes and co-culture of neurons and glial cells) were exposed to RF using an 1800 MHz carrier wave modulated with various environmental signals (GSM: Global System for Mobile Communications, 2G signal), UMTS (Universal Mobile Telecommunications System, 3G signal), LTE (Long-Term Evolution, 4G signal, and Wi-Fi) or unmodulated RF (continuous wave, CW). The specific absorption rates (S.A.R.) used were 1.5 and 6 W/kg during DHM experiments and ranged from 5 to 24 W/kg during the recording of cellular impedance. Cells were continuously exposed for three to five consecutive days while the temporal phenotypic signature of cells behavior was recorded at constant temperature. Statistical analysis of the results does not indicate that RF-EMF exposure impacted the global behavior of healthy, apoptotic, or autophagic cells, even at S.A.R. levels higher than the guidelines, provided that the temperature was kept constant.

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Section: Introductionmentioning

confidence: 99%

Label-Free Study of the Global Cell Behavior during Exposure to Environmental Radiofrequency Fields in the Presence or Absence of Pro-Apoptotic or Pro-Autophagic Treatments

Joushomme

Garenne

Dufossée

et al. 2022

IJMS

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“…The radiations from cell phone affected the semen quality and fertility in males [33]. The exposure to radio frequency leads to proteotoxicity and cancer [34][35][36].…”

Section: Discussionmentioning

confidence: 99%

Electromagnetic Radiations on the Functional Potential of Spermatozoa

Dhasarathan¹,

Mg²,

Aj³

et al. 2021

austinjpublichealthepidemiol

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The growing exposure to digital communication system and tools leads to radiation toxicity to the users. Unaware of the safety measures even a kid at the age of one starts operating digital gadgets emitting Radio frequencyelectromagnetic radiations. Radiations from mobile phones laptops, note pad, Wi-Fi or other devices are reported to be harmful beyond the permissible limit. So there is a growing concern for the overall health, reproductive, and hormonal functions. Experimental studies were conducted by using a Wi-Fi network active laptop and live spermatozoa. The exposure of sperms to the source of EMF showed that the activity of the live sperms got reduced and mortality was observed depending on the exposure duration and the distance from EMF sources. After 5h of exposure to RF-EMF source the semen quality changed when compared with control. The vitality of sperm in the control was 95±1.0 after 2 hr and it was reduced to 60±1.5 percent at a distance of 1cm and 55±2.2 percent at a distance of 10 cm. After 5 hour exposure, the vitality was reduced to 63±1.86 at 1 cm distance and 70±1.42 at 10 cm distance. The reduction in vitality of sperm after exposure to RF-EMF source for 2 hour was 35% at 1cm and 40% at 10cm distance.

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“…The cDNA encoding the nLuc-HSF1 and mNeonG-HSF1 proteins were derived from rLuc-HSF1 and sYFP2-HSF1 expression vectors 13 , in which Renilla Luciferase II and sYFP2 groups were replaced by nLuc and mNeonG, respectively, between BamHI and EcoRI. The HSP90 expression vector was also described in Poque et al 13 .…”

Section: Methodsmentioning

confidence: 99%

“…Assessing whether new RF-EMF technologies induce a cellular stress response under well-controlled exposure conditions is thus one of the mandatory steps on the roadmap for health risk evaluation of 5G signals. In particular, at the molecular level, whether Heat-Shock Proteins (HSP) expression and RAS/MAPK signal transduction pathways are induced has been highly debated due to the central role played by these molecular systems stress responses of cells 13 , 14 . Questions also remain regarding the occurrence of oxidative stress in RF-EMF exposed cells 15 .…”

Section: Introductionmentioning

confidence: 99%

“…Our study used human skin fibroblasts and keratinocytes as cellular models as skin cells will become the primary target tissue of 5G exposure to consider for risk assessment 25 . These cells were continuously or intermittently (5 min ON/10 min OFF) exposed to the 5G-modulated 3.5 GHz RF-EMF signal at 0.25, 1, and 4 W/kg under isothermal conditions for 24 h before HSF1, RAS, ERK, and PML activities were assessed, thanks to well-worn Bioluminescence Resonance Energy Transfer (BRET)-based molecular probes 13 , 14 , 26 .…”

Section: Introductionmentioning

confidence: 99%

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Effects of 5G-modulated 3.5 GHz radiofrequency field exposures on HSF1, RAS, ERK, and PML activation in live fibroblasts and keratinocytes cells

Joushomme

Orlacchio

Patrignoni

et al. 2023

Sci Rep

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The potential health risks of exposure to radiofrequency electromagnetic fields from mobile communications technologies have raised societal concerns. Guidelines have been set to protect the population (e.g. non-specific heating above 1 °C under exposure to radiofrequency fields), but questions remain regarding the potential biological effects of non-thermal exposures. With the advent of the fifth generation (5G) of mobile communication, assessing whether exposure to this new signal induces a cellular stress response is one of the mandatory steps on the roadmap for a safe deployment and health risk evaluation. Using the BRET (Bioluminescence Resonance Energy-Transfer) technique, we assessed whether continuous or intermittent (5 min ON/ 10 min OFF) exposure of live human keratinocytes and fibroblasts cells to 5G 3.5 GHz signals at specific absorption rate (SAR) up to 4 W/kg for 24 h impact basal or chemically-induced activity of Heat Shock Factor (HSF), RAt Sarcoma virus (RAS) and Extracellular signal-Regulated Kinases (ERK) kinases, and Promyelocytic Leukemia Protein (PML), that are all molecular pathways involved in environmental cell-stress responses. The main results are (i), a decrease of the HSF1 basal BRET signal when fibroblasts cells were exposed at the lower SARs tested (0.25 and 1 W/kg), but not at the highest one (4 W/kg), and (ii) a slight decrease of As2O3 maximal efficacy to trigger PML SUMOylation when fibroblasts cells, but not keratinocytes, were continuously exposed to the 5G RF-EMF signal. Nevertheless, given the inconsistency of these effects in terms of impacted cell type, effective SAR, exposure mode, and molecular cell stress response, we concluded that our study show no conclusive evidence that molecular effects can arise when skin cells are exposed to the 5G RF-EMF alone or with a chemical stressor.

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Effects of radiofrequency field exposure on proteotoxic-induced and heat-induced HSF1 response in live cells using the bioluminescence resonance energy transfer technique

Cited by 5 publications

References 49 publications

Label-Free Study of the Global Cell Behavior during Exposure to Environmental Radiofrequency Fields in the Presence or Absence of Pro-Apoptotic or Pro-Autophagic Treatments

Label-Free Study of the Global Cell Behavior during Exposure to Environmental Radiofrequency Fields in the Presence or Absence of Pro-Apoptotic or Pro-Autophagic Treatments

Electromagnetic Radiations on the Functional Potential of Spermatozoa

Effects of 5G-modulated 3.5 GHz radiofrequency field exposures on HSF1, RAS, ERK, and PML activation in live fibroblasts and keratinocytes cells

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