Effects of rat and chicken calcitonin gene-related peptides (CGRP) upon calcium metabolism in chicks

Ancill, A.K.; Bascal, Zainab; Whitaker, G. W.; Dacke, C. G.

doi:10.1016/0167-0115(90)90098-h

Cited by 12 publications

(3 citation statements)

References 19 publications

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“…Recently, many studies have suggested that calcitonin gene-related peptide (CGRP)-positive synovial nerves increase during the late stage of rat adjuvant arthritis (Ahmed et al 1995;Imai et al 1997), and that CGRP stimulates the osteoblast activity in vitro and in vivo (Ancill 1990(Ancill , 1991Kawase et al 1996;Shih and Bernard 1997;Ballica et al 1999;Cornish et al 1999). It is very likely that the sensory fibers release CGRP from their terminals (Kruger et al 1989), and that the ankle joints are supplied with most, if not all, of their sensory fibers via the sciatic nerve.…”

Section: Introductionmentioning

confidence: 99%

Involvement of sensory nerves and immune cells in osteophyte formation in the ankle joint of adjuvant arthritic rats

Nagata

Iijima

2002

Histochem Cell Biol

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To study the mechanism of osteophyte formation in the ankle joints of adjuvant arthritic (AA) rats, the localization of peripheral nerves and immune cells in the synovia were investigated in both axotomized AA rats, whose sciatic nerves were resected before adjuvant injection, and sham-operated ones, using immunohistochemistry for low-affinity nerve growth factor receptor (p75NGFR), growth-associated protein (GAP)-43, calcitonin gene-related peptide (CGRP), helper T cell (W3/25), monocyte/macrophage (ED1), and transforming growth factor (TGF)-β1 and its receptor, TGF-βRII. In sham-operated AA rats, dense plexuses of CGRP-positive fibers were observed in the inflamed synovia close to the osteophytes. Most of the CGRP-positive fibers were also positive for p75NGFR and GAP-43. These fibers appeared to be newly sprouted sensory nerves. In axotomized AA rats, the synovia were supplied with no CGRP-positive fibers and the sizes of the osteophytes were smaller than those in sham-operated animals. The ratio of the number of both W3/25-and ED1-positive cells in the inflamed synovia of sham-operated rats peaked at weeks 2-3 after adjuvant injection. The peak, however, lasted until week 4 in axotomized ones. In both animal groups, the macrophages and the osteoblasts were stained for TGF-β1. The osteoblasts covering the osteophytes were also stained for TGF-βRII. The present findings suggest that the sensory nerves and the macrophages may be involved in osteophyte formation in the ankle joints of AA rats. * Significant difference between the axotomized AA rats and sham-operated AA rats (P<0.001)

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Section: Introductionmentioning

confidence: 99%

Involvement of sensory nerves and immune cells in osteophyte formation in the ankle joint of adjuvant arthritic rats

Nagata

Iijima

2002

Histochem Cell Biol

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“…Es erhöht die Knochenmineralisation in vivo [3,4] und potenziert die u a. durch Bradykinin und "platelet activated factor" induzierte Kapillarpermeabilität [8]. CGRP erhöht auch die c-AMP-Bildung in Osteoblasten bestimmter Spezies [15].…”

Section: Stereologische Berechnung Der Nervenfaserlängeunclassified

Vorkommen und Dynamik peptiderger Nervenfasern im Granulationsgewebe nach Distanzosteosynthese

Wolf

Höcherl

Farkas³

et al. 2001

Der Unfallchirurg

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The transmitters and/or modulators calcitonin gene-related peptide (CGRP), substance P (SP), neuropeptide Y (NPY) and vasointestinal polypeptide (VIP) are supposed to be involved in bone growth, fracture healing and internal remodeling. Immunohistochemical proof of neuropeptide positive fibers in normal bone let us assume that these substances effect the early phase of fracture healing. Exact time of appearance of neuropeptide positive fibers, localisation in the bone, chemospecifity and mode of genesis are unknown so fare. Research was done on a model of distance osteosynthesis of the rabbit tibia. Primary and secondary antibodies were used for indirect immunohistochemical technique. We put more strength on the concrete stereological calculation of length of the nerve fibers than on a conventional statistical evaluation. After histological preparation of tissue specimens from the interfragmental gap and the bone marrow beside the gap the neuropeptides CGRP, SP and NPY were immunohistochemically expressed. Sprouting of CGRP- and SP-positive nerve fibers has its origin in the bone marrow. A vascularisation in the early state of osteoneogenesis after fracture seems impossible without the nerval peptidergic influence and transmission.

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“…The protein binds to cells of the osteosarcoma cell line UMR 106, both through the cell line's endogenous calcitonin receptors and through independent receptors not binding calcitonin 36,37 . CGRP has been repeatedly shown to reduce plasma calcium concentrations in vivo 38–45 and to inhibit osteoclastic bone resorption in vitro 39,40,44‐46 …”

Section: Role Of Neuropeptidesmentioning

confidence: 99%

First Place — Student Research Award 1995: A Mechanism for Sympathectomy‐Induced Bone Resorption in the Middle Ear

Sherman

Chole

1995

Otolaryngol.--head neck surg.

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The results of the in vitro study revealed that substance P at 10(-8) mol/L (11.05 +/- 3.37 micrograms/ml) induced significant calcium release from cultured neonatal mouse calvaria when compared with control bone incubated in base media alone (0.92 +/- 2.85 micrograms/ml, p < 0.01). The process was completely inhibited by 5.0 x 10(-7) indomethacin. The results of the in vivo study showed 6-hydroxydopamine treatment significantly increased both the osteoclast number (NOc/TL = 3.14 +/- 1.33/mm) and the osteoclast surface (OcS/BS = 16.04% +/- 6.95%) of bone when compared with bone from saline-treated controls (NOc/TL = 1.77 +/- 0.79/mm, p < 0.01; OcS/BS = 8.88% +/- 4.15%, p < 0.01). These 6-hydroxydopamine-induced increases were eliminated, however, in animals pretreated with capsaicin before sympathectomy (NOc/TL = 1.86 +/- 0.68/mm, p > 0.05; OcS/BS = 9.92 +/- 3.73, p > 0.05), whereas treatment with capsaicin alone had no effect when compared with bone from saline-treated controls (NOc/TL = 2.02 +/- 0.50/mm, p > 0.05; OcS/BS = 10.28% +/- 2.62%, p > 0.05). Substance P has thus been shown to induce calcium release from membranous bone in vitro, whereas capsaicin, a substance P-specific sensory neurolytic chemical, eliminates the in vivo osteoclast-inductive effects of 6-hydroxydopamine when given 12 hours before treatment. The results indicate that substance P is capable of inducing resorption and that substance P-containing sensory ne

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Effects of rat and chicken calcitonin gene-related peptides (CGRP) upon calcium metabolism in chicks

Cited by 12 publications

References 19 publications

Involvement of sensory nerves and immune cells in osteophyte formation in the ankle joint of adjuvant arthritic rats

Involvement of sensory nerves and immune cells in osteophyte formation in the ankle joint of adjuvant arthritic rats

Vorkommen und Dynamik peptiderger Nervenfasern im Granulationsgewebe nach Distanzosteosynthese

First Place — Student Research Award 1995: A Mechanism for Sympathectomy‐Induced Bone Resorption in the Middle Ear

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