Effects of Raw Potato Starch with High Resistant Starch Levels on Cecal Fermentation Properties in Rats

Nagata, Ryuji; Innami, Naoya; Pelpolage, Samanthi; Shimada, Kaoru; Koaze, Hiroshi; Tani, Masayuki; Han, Kyu‐Ho; Fukushima, Michihiro

doi:10.3177/jnsv.65.s192

Cited by 6 publications

(14 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In our experiment, the increased cecal amylose concentration in LRDS-fed goats is speculated to be related to the more uniform distribution at each site of the gastrointestinal tract (GIT). Previous studies show that high amylose increased cecal SCFAs concentrations in rats and piglets ( Nagata et al, 2019 ; Gao et al, 2020 ). Our study further verified that feeding diets with different RDS levels could alter the hindgut digesta composition of dairy goats thereby influencing the synthesis of SCFAs.…”

Section: Discussionmentioning

confidence: 88%

A Metagenomic Insight Into the Hindgut Microbiota and Their Metabolites for Dairy Goats Fed Different Rumen Degradable Starch

Han

Xuexin

et al. 2021

Front. Microbiol.

View full text Add to dashboard Cite

High starch diets have been proven to increase the risk of hindgut acidosis in high-yielding dairy animals. As an effective measurement of dietary carbohydrate for ruminants, studies on rumen degradable starch (RDS) and the effects on the gut microbiota diversity of carbohydrate-active enzymes (CAZymes), and Kyoto Encyclopedia of Genes and Genomes (KEGG) Orthology functional categories are helpful to understand the mechanisms between gut microbiota and carbohydrate metabolism in dairy goats. A total of 18 lactating goats (45.8 ± 1.54 kg) were randomly divided equally into three dietary treatments with low dietary RDS concentrations of 20.52% (LRDS), medium RDS of 22.15% (MRDS), and high RDS of 24.88% (HRDS) on a DM basis for 5 weeks. Compared with the LRDS and MRDS groups, HRDS increased acetate molar proportion in the cecum. For the HRDS group, the abundance of family Ruminococcaceae and genus Ruminococcaceae UCG-010 were significantly increased in the cecum. For the LRDS group, the butyrate molar proportion and the abundance of butyrate producer family Bacteroidale_S24-7, family Lachnospiraceae, and genus Bacteroidale_S24-7_group were significantly increased in the cecum. Based on the BugBase phenotypic prediction, the microbial oxidative stress tolerant and decreased potentially pathogenic in the LRDS group were increased in the cecum compared with the HRDS group. A metagenomic study on cecal bacteria revealed that dietary RDS level could affect carbohydrate metabolism by increasing the glycoside hydrolase 95 (GH95) family and cellulase enzyme (EC 3.2.1.4) in the HRDS group; increasing the GH13_20 family and isoamylase enzyme (EC 3.2.1.68) in the LRDS group. PROBIO probiotics database showed the relative gene abundance of cecal probiotics significantly decreased in the HRDS group. Furthermore, goats fed the HRDS diet had a lower protein expression of Muc2, and greater expression RNA of interleukin-1β and secretory immunoglobulin A in cecal mucosa than did goats fed the LRDS diet. Combined with the information from previous results from rumen, dietary RDS level altered the degradation position of carbohydrates in the gastrointestinal (GI) tract and increased the relative abundance of gene encoded enzymes degrading cellulose in the HRDS group in the cecum of dairy goats. This study revealed that the HRDS diet could bring disturbances to the microbial communities network containing taxa of the Lachnospiraceae and Ruminococcaceae and damage the mucus layer and inflammation in the cecum of dairy goats.

show abstract

Section: Discussionmentioning

confidence: 88%

A Metagenomic Insight Into the Hindgut Microbiota and Their Metabolites for Dairy Goats Fed Different Rumen Degradable Starch

Han

Xuexin

et al. 2021

Front. Microbiol.

View full text Add to dashboard Cite

show abstract

“…Similarly, we saw an increase in butyrate production and decreases in branched-chain SCFAs, indicative of a shift to saccharolytic fermentation and a decrease in proteolytic fermentation (branched-chain SCFAs) ( 53 , 54 ). Consumption of resistant starches has also been associated with a decrease in fecal pH ( 5 , 6 ) which can favor butyrate forming taxa ( 55 ). Others have also reported increased butyrate ( 5 , 6 , 56 ), and enlargement of the cecum in rodent studies ( 28 , 45 , 46 ).…”

Section: Discussionmentioning

confidence: 99%

“…Consumption of resistant starches has also been associated with a decrease in fecal pH ( 5 , 6 ) which can favor butyrate forming taxa ( 55 ). Others have also reported increased butyrate ( 5 , 6 , 56 ), and enlargement of the cecum in rodent studies ( 28 , 45 , 46 ). Decreased fecal pH ( 20 , 57 , 58 ), increased butyrate production, reduced levels of branched-chain SCFAs ( 9 , 11 , 58 – 60 ), and increased colon and cecum weight have also been observed in pigs fed with RS ( 11 , 57 ), likely the result of increased levels of undigested fiber/RS reaching the cecum.…”

Section: Discussionmentioning

confidence: 99%

Resistant Potato Starch Alters the Cecal Microbiome and Gene Expression in Mice Fed a Western Diet Based on NHANES Data

et al. 2022

View full text Add to dashboard Cite

Several studies indicate that the four major types of resistant starch (RS1-4) are fermented in the cecum and colon to produce short-chain fatty acids (SCFAs) and can alter the microbiome and host physiology. However, nearly all these studies were conducted in rodents fed with a diet that does not approximate what is typically consumed by humans. To address this, mice were fed a Total Western Diet (TWD) based on National Health and Nutrition Examination Survey (NHANES) data that mimics the macro and micronutrient composition of a typical American diet for 6 weeks and then supplemented with 0, 2, 5, or 10% of the RS2, resistant potato starch (RPS), for an additional 3 weeks. The cecal microbiome was analyzed by 16S sequencing. The alpha-diversity of the microbiome decreased with increasing consumption of RPS while a beta-diversity plot showed four discreet groupings based on the RPS level in the diet. The relative abundance of various genera was altered by feeding increasing levels of RPS. In particular, the genus Lachnospiraceae NK4A136 group was markedly increased. Cecal, proximal, and distal colon tissue mRNA abundance was analyzed by RNASeq. The cecal mRNA abundance principal component analysis showed clear segregation of the four dietary groups whose separation decreased in the proximal and distal colon. Differential expression of the genes was highest in the cecum, but substantially decreased in the proximal colon (PC) and distal colon (DC). Most differentially expressed genes were unique to each tissue with little overlap in between. The pattern of the observed gene expression suggests that RPS, likely through metabolic changes secondary to differences in microbial composition, appears to prime the host to respond to a range of pathogens, including viruses, bacteria, and parasites. In summary, consumption of dietary RPS led to significant changes to the microbiome and gene expression in the cecum and to a lesser extent in the proximal and distal colon.

show abstract

“…The dosages of PS and Ca‐PS in diets were determined in the authors' previous studies, in which rats were fed 30% PS in the diet. [ 19,20 ] Calorie content in the CON diet was described in a previous report. [ 21 ] The rats had access to food and water ad libitum.…”

Section: Methodsmentioning

confidence: 99%

Effect of Calcium‐Fortified Potato Starch on Cecal Fermentation and Fat Accumulation in Rats

et al. 2020

Self Cite

View full text Add to dashboard Cite

Calcium‐fortified potato starch (Ca‐PS) is fed to rats for four weeks, and the effects on cecal fermentation and lipid metabolism are evaluated. The relative abundance of Bifidobacterium pseudolongum in rat cecum is higher in the PS and Ca‐PS groups than in the control group. Cecal acetate content is higher in the Ca‐PS group than in the control, high‐amylose cornstarch (HAS), and PS groups, and is positively correlated with the relative abundance of B. pseudolongum. The relative abundance of the Clostridiaceae family is higher in the PS and Ca‐PS groups than in the control and HAS groups, and is positively correlated with the cecal n‐butyrate content and the molar proportion of n‐butyrate in total‐SCFA. Furthermore, the consumption of PS and Ca‐PS reduces the serum and hepatic triglyceride levels and mesenteric adipocyte area in rats. Therefore, Ca‐PS may have beneficial effects that enhance cecal fermentation, especially in terms of cecal acetate production, which further suppresses fat accumulation. This is the first study to report the physiological effects of Ca‐PS.

show abstract

Effects of Raw Potato Starch with High Resistant Starch Levels on Cecal Fermentation Properties in Rats

Cited by 6 publications

References 27 publications

A Metagenomic Insight Into the Hindgut Microbiota and Their Metabolites for Dairy Goats Fed Different Rumen Degradable Starch

A Metagenomic Insight Into the Hindgut Microbiota and Their Metabolites for Dairy Goats Fed Different Rumen Degradable Starch

Resistant Potato Starch Alters the Cecal Microbiome and Gene Expression in Mice Fed a Western Diet Based on NHANES Data

Effect of Calcium‐Fortified Potato Starch on Cecal Fermentation and Fat Accumulation in Rats

Contact Info

Product

Resources

About