Effects of reactive oxygen species on sperm function

Guthrie, Helen A.; Welch, G. Rickey

doi:10.1016/j.theriogenology.2012.05.002

Cited by 232 publications

(159 citation statements)

References 42 publications

Supporting

Mentioning

143

Contrasting

Unclassified

Order By: Relevance

“…in the T100 group were lower than those reported by Guthrie and Welch (2007) [10]. These differences may be explained by the differences between the CellROX ® and BODIPY methods.…”

Section: Discussioncontrasting

confidence: 56%

“…Besides, sperm mitochondria produces the most ROS [4], which trigger lipid peroxidation of polyunsaturated fatty acids in the plasma membrane [3,5]. Lipid peroxidation, in turn, reduces plasma membrane fluidity and, as a result, it prejudices sperm function, culminating in decreased sperm motility [6][7][8] and increased rates of sperm DNA fragmentation [9], cellular apoptosis [2,8,10,11] and morphologically abnormal sperm [2,6].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

An Efficient Technique to Detect Sperm Reactive Oxygen Species: The CellRox Deep Red® Fluorescent Probe

Alves¹,

Andrade²,

Arruda³

et al. 2015

Biochem Physiol

View full text Add to dashboard Cite

“…in the T100 group were lower than those reported by Guthrie and Welch (2007) [10]. These differences may be explained by the differences between the CellROX ® and BODIPY methods.…”

Section: Discussioncontrasting

confidence: 56%

Section: Introductionmentioning

confidence: 99%

An Efficient Technique to Detect Sperm Reactive Oxygen Species: The CellRox Deep Red® Fluorescent Probe

Alves¹,

Andrade²,

Arruda³

et al. 2015

Biochem Physiol

View full text Add to dashboard Cite

“…Previous reports have indicated that there is a negative correlation between MDA production and sperm viability and sperm function (Aitken et al, 1989;Guthrie & Welch, 2012). The results of the present study showed that the addition of ROM and its combination with GSH probably protected the lipids, proteins and other ultra-structure compounds of the cell membrane against cryo-damage.…”

Section: Discussionsupporting

confidence: 58%

Effect of rosemary (Rosmarinus officinalis) extracts and glutathione antioxidants on bull semen quality after cryopreservation

Kia

Olfati-Karaji

Hoseinkhani

et al. 2014

Span. j. agric. res.

View full text Add to dashboard Cite

The present study determined the effects of the addition of rosemary extract (ROM), glutathione (GSH), and their combination (ROM + GSH) to freezing extender on the quality of bull semen after cryopreservation. Before cryoperservation, the samples were diluted in a tris-egg yolk (TEY) extender containing 5 mM GSH (treatment I), 5 or 10 g L–1 ROM (treatments II and III), and ROM with GSH (5 mM GSH with 5 or 10 g L–1 of ROM) (treatments IV and V). An extender containing no antioxidants (non-ROM/GSH-treated) served as control group. Kinematic parameters were evaluated by means of a computer-assisted semen analysis (CASA). The viability and membrane integrity of the sperm were assessed using eosin-nigrosin stain and the hypo-osmotic swelling test (HOST) at 0 and 2 h after freezethawing. Lipooxidative parameters, superoxide dismutase, and glutathione peroxidase (GPx) activity were assessed after thawing. Treatment III showed positive effects for total motility (TM) (p < 0.01), average path velocity (VAP) (p < 0.001), viability (p < 0.01) and HOST (p < 0.01); however, lipid peroxidation (LPO) decreased (p < 0.05) and GPx activity increased (p < 0.05) immediately after thawing compared to the control. The TM (p < 0.01), VAP (p < 0.01), viability (p < 0.01), HOST (p < 0.01) decreased in LPO (p < 0.01) and GPx activity (p < 0.05) for treatment V and the viability and GPx activity (p < 0.05) for treatment I were significantly higher than for the control group at 2 h after thawing. It was concluded that the inclusion of ROM and its combination with GSH improves the post-thaw quality of bull semen.

show abstract

“…In our study the SOD activity did not change depending on the extender, which may suggest that it is more resistant to cryogenic damage than GPx. Moreover, its low activity in the spermatozoa following thawing, as compared with that of the spermatozoa prior to freezing, may indicate a low level of ROS generation, which was previously suggested by Guthrie and Welch (2012).…”

Section: Discussionmentioning

confidence: 75%

“…Numerous studies have demonstrated that liquid storage or cryopreservation of boar semen induces marked changes in the structural and functional integrity of the sperm membranes (Roca et al 2006, Gączarzewicz et al 2010. During the cryopreservation procedure, damage to the sperm mitochondria occurs, which in turn leads to the disruption of the oxidative phosphorylation process, and a reduction in post-thaw sperm motility (Johnson et al 2000, Peñ a et al 2011, Guthrie and Welch 2006, 2012. Hence, in our study, irrespective of the extender used, a reduction in mitochondrial functionality was also observed, which was reflected in a reduction in mitochondrial membrane potential, ATP content and the motility of the thawed spermatozoa.…”

Section: Discussionmentioning

confidence: 99%

Effects of storage in different semen extenders on the pre-freezing and post-thawing quality of boar spermatozoa

Dziekońska¹,

Zasiadczyk²,

Lecewicz³

et al. 2015

Polish Journal of Veterinary Sciences

View full text Add to dashboard Cite

The aim of this study was to investigate the effects of storage of semen in different commercial extenders on the pre-freezing and post-thawing quality of boar spermatozoa. Semen was diluted in BTS, Androhep (AH) and Gedil (GD), stored for 24 h at 17 o C, and then frozen in accordance with the cryopreservation protocol. Analyses of the quality of spermatozoa included: motility, normal apical ridge (NAR) acrosome, plasma membrane integrity (PMI), mitochondrial membrane potential (MMP), measurements of ATP content and activity of superoxidase dismutase (SOD) and glutathione peroxidase (GPx). Prior to the freezing process, no significant effect of the extender on the sperm quality parameters was noted. After thawing the spermatozoa it was demonstrated that the type of extender used influenced PMI, MMP, ATP content and activity of GPx. In the AH extender the percentage of spermatozoa with PMI and ATP content in spermatozoa was significantly higher (P<0.05) as compared to the BTS or GD extenders. In addition, semen stored in the AH was characterised by a statistically higher (P<0.05) percentage of spermatozoa with MMP and increased activity of GPx as compared with the BTS. The results obtained indicate that for the cryopreservation process, boar spermatozoa stored for 24 hours in liquid state can be used. However, the type of extender used prior to freezing may have a significant effect on the post-thawing quality of the spermatozoa. The AH extender better secured the quality of thawed boar spermatozoa as compared with the BTS or GD.

show abstract

Effects of reactive oxygen species on sperm function

Cited by 232 publications

References 42 publications

An Efficient Technique to Detect Sperm Reactive Oxygen Species: The CellRox Deep Red® Fluorescent Probe

An Efficient Technique to Detect Sperm Reactive Oxygen Species: The CellRox Deep Red® Fluorescent Probe

Effect of rosemary (Rosmarinus officinalis) extracts and glutathione antioxidants on bull semen quality after cryopreservation

Effects of storage in different semen extenders on the pre-freezing and post-thawing quality of boar spermatozoa

Contact Info

Product

Resources

About