Effects of reactive oxygen species generation induced by Wonju City particulate matter on mitochondrial dysfunction in human middle ear cell

Lee, Su Hoon; Ha, Sun Mok; Jeong, Min Chang; Polo, Carlos Noriega; Seo, Young Joon; Kim, Seong Heon

doi:10.1007/s11356-021-14216-7

Cited by 3 publications

(2 citation statements)

References 42 publications

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“…4A and 4B ), indicating that PM 10 depolarized the mitochondrial membrane. PM can alter mitochondrial membrane polarization by inducing ROS production ( Kyung et al, 2012 ; Lee et al, 2021 ). In mitochondrial damage by PM, NADH dehydrogenase gene expression levels were decreased dynamically following mitochondrial dysfunction ( Li et al, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

Particulate matter 10 induces oxidative stress and apoptosis in rhesus macaques skin fibroblast

Lee,

Kwon,

et al. 2023

PeerJ

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Background Particulate matter (PM) is a major air pollutant that affects human health worldwide. PM can pass through the skin barrier, thus causing skin diseases such as heat rash, allergic reaction, infection, or inflammation. However, only a few studies have been conducted on the cytotoxic effects of PM exposure on large-scale animals. Therefore, herein, we investigated whether and how PM affects rhesus macaque skin fibroblasts. Methods Rhesus macaque skin fibroblasts were treated with various concentrations of PM10 (1, 5, 10, 50, and 100 μg/mL) and incubated for 24, 48, and 72 h. Then, cell viability assay, TUNEL assay, and qRT-PCR were performed on the treated cells. Further, the reactive oxygen species, glutathione, and cathepsin B levels were determined. The MTT assay revealed that PM10 (>50 μg/mL) proportionately reduced the cell proliferation rate. Results PM10 treatment increased TUNEL-positive cell numbers, following the pro-apoptosis-associated genes (CASP3 and BAX) and tumor suppressor gene TP53 were significantly upregulated. PM10 treatment induced reactive oxidative stress. Cathepsin B intensity was increased, whereas GSH intensity was decreased. The mRNA expression levels of antioxidant enzyme-related genes (CAT, GPX1 and GPX3) were significantly upregulated. Furthermore, PM10 reduced the mitochondrial membrane potential. The mRNA expression of mitochondrial complex genes, such as NDUFA1, NDUFA2, NDUFAC2, NDUFS4, and ATP5H were also significantly upregulated. In conclusion, these results showed that PM10 triggers apoptosis and mitochondrial damage, thus inducing ROS accumulation. These findings provide potential information on the cytotoxic effects of PM10 treatment and help to understand the mechanism of air pollution-induced skin diseases.

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Section: Discussionmentioning

confidence: 99%

Particulate matter 10 induces oxidative stress and apoptosis in rhesus macaques skin fibroblast

Lee,

Kwon,

et al. 2023

PeerJ

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“…Otitis media has one of the highest disease prevalence rates in the world [1,2]. However, otoscopic screening is highly subspecialized, creating diagnostic difficulties for primary care providers who are relatively inaccurate in providing otologic diagnoses.…”

Section: Introductionmentioning

confidence: 99%

Deep Learning Techniques for Ear Diseases Based on Segmentation of the Normal Tympanic Membrane

Park¹,

Jeon

Kong

et al. 2023

Clin Exp Otorhinolaryngol

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Background: Otitis media is a common infection affecting people worldwide. Owing to the limited number of ear specialists and rapid development in telemedicine, several trials have been conducted for developing novel diagnostic strategies to improve the diagnostic accuracy and screening of patients afflicted with otologic diseases based on abnormal otoscopic findings. Although these strategies have demonstrated a high diagnostic accuracy for the tympanic membrane (TM), the insufficient explainability of such techniques limits their deployment in clinical practices. Methods: Herein, we used a deep convolutional neural network (CNN) model based on the segmentation of a normal TM into five substructures (malleus, umbo, cone of light, pars flaccida, and annulus) to identify abnormalities in otoscopic ear images. The Mask R-CNN algorithm learned the labeled images. Subsequently, the values were combined according to the five substructures using a three-layer fully connected neural network to determine whether an ear disease was present. Results: We obtained the receiver operating characteristic (ROC) curves of the optimal condition for the presence or absence of eardrum diseases according to each substructure or a combination of substructures. The results indicated that the highest area under the curve ranged from a 0.911 true-positive rate in the ROC curve combined with malleus, cone of light, and umbo, compared with the corresponding range of 0.737–0.873 in each substructure. Thus, an algorithm using these five important normal anatomical structures could prove to be explainable and effective in screening abnormal TMs.Conclusion: This automated algorithm can improve the diagnostic accuracy to discriminate between normal and abnormal TMs and facilitate appropriate and timely referral consultations to improve the quality of life in public healthcare.

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Integrated mitoepigenetic signalling mechanisms associated with airborne particulate matter exposure: A cross-sectional pilot study

Mishra

Bhargava

Kumari

et al. 2022

Atmospheric Pollution Research

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Effects of reactive oxygen species generation induced by Wonju City particulate matter on mitochondrial dysfunction in human middle ear cell

Cited by 3 publications

References 42 publications

Particulate matter 10 induces oxidative stress and apoptosis in rhesus macaques skin fibroblast

Particulate matter 10 induces oxidative stress and apoptosis in rhesus macaques skin fibroblast

Deep Learning Techniques for Ear Diseases Based on Segmentation of the Normal Tympanic Membrane

Integrated mitoepigenetic signalling mechanisms associated with airborne particulate matter exposure: A cross-sectional pilot study

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