Effects of Reagent and Instrument on Prothrombin Times, Activated Partial Thromboplastin Times and Patient/Control Ratios

Naghibi, F; Han, Yangsook; Dodds, W J; Lawrence, Charles E.

doi:10.1055/s-0038-1647515

Cited by 21 publications

(14 citation statements)

References 8 publications

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“…First, the relationship between coagulation factors and PT or aPTT is nonlinear, such that there is an exponential relationship between clotting factor concentrations and test results . Next, mildly abnormal test results can occur in patients with biologically normal coagulation . Also, PT and aPTT tests will overestimate deficiencies in the upper limb of the cascade and underestimate deficiencies in the lower limb.…”

Section: Coagulopathy and The Risk Of Bleedingmentioning

confidence: 99%

Controversies in the use of fresh frozen plasma in critically ill small animal patients

Beer

Silverstein

2015

J Vet Emergen Crit Care

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The use of FFP in critically ill patients remains controversial. In the absence of clinical bleeding or a risk for clinical bleeding associated with a planned procedure, treatment use of FFP is not recommended in human patients. There are insufficient data in critically ill animals to enable formulation of recommendations. Further research is warranted in dogs and cats to establish evidence-based guidelines.

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Section: Coagulopathy and The Risk Of Bleedingmentioning

confidence: 99%

Controversies in the use of fresh frozen plasma in critically ill small animal patients

Beer

Silverstein

2015

J Vet Emergen Crit Care

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“…EVANS & FLYNN (1992), utilizando diferentes tempos de incubação (10 e 2 minutos) e diferentes métodos automáticos ("Coagulyser" e "KC 10 Coagulometer"), obtiveram valores máximos e mínimos (Tabela 1) inferiores aos encontrados neste estudo. BADYLAK & VAN VLEET (1981), DUNCAN & PRASSE (1982), DUNCAN et al (1994), DODDS (1997) GREENE et al (1981), DUNCAN et al (1994), MONCE et al (1995) e TSENG et al (2001, uma vez que estes valores estão relacionados a vários fatores, como: reagentes, temperatura e método utilizado (NAGHIBI et al, 1988;HASSOUNA, 1993;MONCE et al, 1995). Também os reagentes comerciais utilizados para os testes de coagulação foram desenvolvidos em condições ótimas para o plasma humano.…”

Section: Resultsunclassified

“…Os "kits" permitem a determinação quantitativa manual ou automática do TP e do TTPa. Estudos recentes vêm utilizando métodos automáticos e comparando-os com os métodos manuais já utilizados (MISCHKE & NOLTE, 1997;MISCHKE, 2001;TSENG et al, 2001) (NAGHIBI et al, 1988;HASSOUNA, 1993;MONCE et al, 1995). Além destas variações, existem aquelas relacionadas a espécie, raça, sexo, idade, dieta, estado de nutrição entre outros que podem influenciar nas determinações (DUNCAN et al, 1994).…”

Section: Os Fatores De Coagulação São Parte Integrante Da Hemostasia unclassified

Valores de referência do tempo de protrombina (TP) e tempo de tromboplastina parcial ativada (TTPa) em cães

et al. 2005

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Os fatores de coagulação são parte integrante da hemostasia normal, e tanto as coagulopatias hereditárias como adquiridas que envolvem este sistema são de grande importância veterinária, geralmente evidenciadas por manifestações clínicas, tais como, petéquias, equimoses, hematomas e sangramentos tardios. O presente trabalho teve por objetivo a determinação dos valores de referência do tempo de protrombina (TP) e tempo de tromboplastina parcial ativada (TTPa), por métodos manuais. Para tanto, utilizou-se "kits" para dosagens humanas, devido à inexistência de produtos similares de uso específico veterinário. Foram utilizados 40 cães clinicamente sadios, sem raça definida, machos ou fêmeas, de diferentes idades. As amostras sanguíneas foram de 2,5ml cada, colhidas por venopunção cefálica e acondicionadas em tubos de centrífuga contendo 0,25ml de citrato de sódio a 3,8%. O plasma foi imediatamente separado por centrifugação e as determinações de TP e TTPa foram realizadas utilizando-se "kits" comerciais "HemoStat Thromboplastin-SIª" e "HemoStat aPTT-El b", respectivamente. Os resultados obtidos foram de 6,87 ± 1,4 segundos para o TP com valores mínimo e máximo de 4,07 e 9,67, respectivamente, e de 15,10 ± 1,6 segundos para TTPa com valores mínimo e máximo de 11,9 e 18,3, respectivamente. Conclui-se, que os valores obtidos neste trabalho podem ser utilizados como referência. Os reagentes utilizados para plasma humano podem ser empregados para o plasma de cães.

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“…One concern often expressed with POC devices is the lack of quality standards (Oral Anticoagulation Monitoring Study Group, 2001). Quality control is part of the regulatory requirements in a laboratory and medical and laboratory instrumentation are enrolled in quality assurance programmes (Naghibi et al, 1988). One way of overcoming this may be to have regular QC on the POC device performed by the laboratory staff on a permanent basis.…”

Section: Discussionmentioning

confidence: 99%

Point-of-care testing of neonatal coagulation

Tan¹,

Booth²,

Newell³

et al. 2006

Clin Lab Haematol

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The aim of this validation study was to compare prothrombin time (PT) and activated partial thromboplastin time (APTT) results from a point-of-care testing (POCT) device (Rapidpoint Coag) with those from standard laboratory tests. The subjects were newborn infants needing coagulation screen for any clinical indications within a regional neonatal intensive care unit. The level of agreement between POCT and laboratory measurements of PT and APTT was determined. For PT: the bias was from -7.6 to 12.4 s and precision was 5.0 s. For the detection of prolonged PT at a level of 16 s, the sensitivity was 0.70, specificity was 0.57 and the positive predictive value (PPV) was 0.62. For APTT: the bias was from -39.1 to 23.7 s, and precision was 15.7 s. For the detection of prolonged APTT at a level of 55 s, the sensitivity was 0.80, specificity was 0.95 and the PPV was 0.80. The POCT device tested has limited utility as a cot-side device for screening for a prolongation of the APTT in the newborn but is not sensitive for screening for prolongation of the PT.

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Effects of Reagent and Instrument on Prothrombin Times, Activated Partial Thromboplastin Times and Patient/Control Ratios

Cited by 21 publications

References 8 publications

Controversies in the use of fresh frozen plasma in critically ill small animal patients

Controversies in the use of fresh frozen plasma in critically ill small animal patients

Valores de referência do tempo de protrombina (TP) e tempo de tromboplastina parcial ativada (TTPa) em cães

Point-of-care testing of neonatal coagulation

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