Effects of renal denervation on the expression profile of circular RNA in the serum of patients with resistant hypertension

Cai, Wenqin; Li, Jing-Ting; Su, Jinzi

doi:10.1016/j.hjc.2021.06.007

“…In addition to participating in the regulation of sympathetic inflammation, miRNAs participate in the cholinergic anti-inflammatory pathway [ 40 , 55 ]. CircRNAs are also regulated by sympathetic nerves; Cai W et al found that renal SD in patients with persistent hypertension affects the expression of circRNAs [ 56 ]. Interestingly, it seems that ncRNAs also participate in the activity of nerves.…”

Section: Discussionmentioning

confidence: 99%

Deregulated RNAs involved in sympathetic regulation of sepsis-induced acute lung injury based on whole transcriptome sequencing

Zhang

¹

,

Zhang

²

,

Nie

³

et al. 2022

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Sympathetic nerves play essential roles in the regulation of lung inflammation, and we investigated the effect of sympathetic denervation (SD) on sepsis-induced acute lung injury (ALI) in mice. Mice were randomized to the control, SD, ALI and SD + ALI, groups. SD and ALI were established through intratracheal 6-hydroxydopamine and intraperitoneal lipopolysaccharide, respectively. Models and gene expressions levels were evaluated by HE staining, ELISA, Western blotting and RT-qPCR. RNA extraction, whole transcriptome sequencing and subsequent biostatistical analysis were performed. Sympathetic denervation in the lungs significantly attenuated lung TNF-ɑ and norepinephrine expression, alleviated sepsis-induced acute lung injury and inhibited NF-κB signaling. Compared with the ALI group, the SD + ALI group exhibited 629 DE circRNAs, 269 DE lncRNAs,7 DE miRNAs and 186 DE mRNAs, respectively. Some DE RNAs were validated by RT-qPCR. CircRNA–miRNA–mRNA regulatory networks in the SD + ALI group revealed enrichment of the B-cell receptor signaling pathway, IL-17 signaling pathway, neuroactive ligand–receptor interaction, CAM, primary immunodeficiency, and cytokine–cytokine receptor interaction terms. The lncRNA-miRNA-mRNA network also revealed inflammation–related signaling pathways. Taken together, based on the successfully established models of SD and ALI, we show here that sympathetic nerves may regulate sepsis-induced ALI supposedly by affecting the expression of circRNAs, lncRNAs, miRNAs, and mRNAs in the lungs. These results may allow for further exploration of the roles of pulmonary sympathetic nerves in sepsis-induced ALI.

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“…The immune and the nervous systems intensely interact and mutually control each other [24]. In peripheral tissues, the sympathetic nervous system has been demonstrated to regulate key processes of immune cell function, such as the release of monocytes from the bone marrow compartment [25] tissue programming of macrophages [27], the cytokine expression profile of various immune cells [28], and the antibody production of B cells [29]. A detailed picture of the signalling pathways and molecules involved that execute these diverse functions is only gradually evolving.…”

Section: Discussionmentioning

confidence: 99%

Sequencing of RNAs involved in sepsis-induced myocardial injury: role of Local sympathetic denervation

Alcantara¹

2022

AJBM

0

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Sepsis has been a serious public health concern for a long time and is characterized by disrupted development of specific diagnostic criteria and novel therapies for sepsis is critically dependent on an understanding of the basic mechanisms immune homeostasis accompanied by infection and multiple organ dysfunction as determined assessed by the Sequential Organ Failure. Adult (4 to 6 months) male C57BL/6 mice, animal set in three groups, control group, Cecal Ligation and Puncture (CLP) group, CLP+ Local sympathetic denervation group 8 mice in each group. CLP model for created sepsis and local sympathetic denervation procedure for alleviated the effects of sepsis. Assessment of cardiac function by Millar Instruments, immunofluorescence staining, enzyme-linked immunosorbent assay (ELISA) and RNA extraction and sequencing. Our findings showed that the myocardial injury score and TNF-α level in both tissue and serum were significantly increased in the CLP group. CLP with local sympathetic denervation group mice had lower scores of myocardial injuries. Sepsis augments MCP-1 and KC level in myocardium. After 48 hours after CLP, the myocardial injury score. CLP with local sympathetic denervation alleviates sepsis-induced myocardial injury by inhibiting the NF-κB signaling pathway. Moreover, compared with the Ctrl group, the CLP with local sympathetic denervation group obtained fewer ncRNAs and mRNAs. Inconclusions, the NF-κB signaling pathway is associated with sepsis-induced myocardial injury, and local sympathetic denervation can relieve sepsis-induced myocardial injury by regulating the ncRNAs and mRNAs.

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“…The immune and the nervous systems intensely interact and mutually control each other [24]. In peripheral tissues, the sympathetic nervous system has been demonstrated to regulate key processes of immune cell function, such as the release of monocytes from the bone marrow compartment [25] tissue programming of macrophages [27], the cytokine expression profile of various immune cells [28], and the antibody production of B cells [29]. A detailed picture of the signalling pathways and molecules involved that execute these diverse functions is only gradually evolving.…”

Section: Discussionmentioning

confidence: 99%

Untitled

2022

AJBM

0

View full text Add to dashboard Cite

Sepsis has been a serious public health concern for a long time and is characterized by disrupted development of specific diagnostic criteria and novel therapies for sepsis is critically dependent on an understanding of the basic mechanisms immune homeostasis accompanied by infection and multiple organ dysfunction as determined assessed by the Sequential Organ Failure. Adult (4 to 6 months) male C57BL/6 mice, animal set in three groups, control group, Cecal Ligation and Puncture (CLP) group, CLP+ Local sympathetic denervation group 8 mice in each group. CLP model for created sepsis and local sympathetic denervation procedure for alleviated the effects of sepsis. Assessment of cardiac function by Millar Instruments, immunofluorescence staining, enzyme-linked immunosorbent assay (ELISA) and RNA extraction and sequencing. Our findings showed that the myocardial injury score and TNF-α level in both tissue and serum were significantly increased in the CLP group. CLP with local sympathetic denervation group mice had lower scores of myocardial injuries. Sepsis augments MCP-1 and KC level in myocardium. After 48 hours after CLP, the myocardial injury score. CLP with local sympathetic denervation alleviates sepsis-induced myocardial injury by inhibiting the NF-κB signaling pathway. Moreover, compared with the Ctrl group, the CLP with local sympathetic denervation group obtained fewer ncRNAs and mRNAs. Inconclusions, the NF-κB signaling pathway is associated with sepsis-induced myocardial injury, and local sympathetic denervation can relieve sepsis-induced myocardial injury by regulating the ncRNAs and mRNAs.

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Effects of renal denervation on the expression profile of circular RNA in the serum of patients with resistant hypertension

Cited by 5 publications

References 31 publications

Deregulated RNAs involved in sympathetic regulation of sepsis-induced acute lung injury based on whole transcriptome sequencing

Deregulated RNAs involved in sympathetic regulation of sepsis-induced acute lung injury based on whole transcriptome sequencing

Sequencing of RNAs involved in sepsis-induced myocardial injury: role of Local sympathetic denervation

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