Effects of replacing dietary starch with neutral detergent–soluble fibre on ruminal fermentation, microbial synthesis and populations of ruminal cellulolytic bacteria using the rumen simulation technique (<scp>RUSITEC</scp>)

Zhao, Xianghui; Liu, C. J.; Liu, Y.; Li, C. Y.; Yao, Junhu

doi:10.1111/jpn.12025

Cited by 32 publications

(34 citation statements)

References 38 publications

(52 reference statements)

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“…Mrazek et al (23), identificó que los aislamientos de Pseudobutyrivibrio ruminis y Butyrivibrio proteoclasticum encontrados en el rumen bovino, producen succinato y propanoato, pero no producen butirato y acetato, en contraste a la cepa tipo de B. fibrisolvens, el cual produce ácidos láctico y butírico y más baja cantidad de ácidos fórmico y succínico. Las especies de este género pertenecen al grupo XIVa de la Subphylum Clostridium y toman parte en funciones importantes del ecosistema ruminal, incluyendo el proceso de proteólisis para la degradación de la fibra y en la biohidrogenación de ácidos grasos (13,21,24). Estos también tienen la habilidad para hidrogenar los ácidos vaccénico y linoleico, mediante la actividad enzimática; el ácido linoleico isomerasa y el ácido linoleico reductasa confieren especificidad al uso del ácido linoleico en la formación del ácido linoleico conjugado (CLA) (6).…”

Section: Discussionunclassified

“…Su concentración se relaciona directamente con la dieta suministrada a los animales, la cual es rica en ácidos linoleicos y alfalinoléico, proteína y fibra y requiere activa participación de los microorganismos ruminales encargados de dicho proceso, como es el caso de la bacteria ruminal B. fibrisolvens (6,7,13,24). Aunque durante este estudio no se realizó una cuantificación directa de la producción del CLA (debido a la utilización del sistema de simulación ruminal in vitro) en carne y productos lácteos, fue posible observar que la concentración de ácido vaccénico en Rusitec, fue directamente proporcional a la densidad poblacional de B. fibrisolvens conforme a la suplementación del pasto kikuyo con harina dairy products, it was possible to observe that the vaccenic acid concentration in Rusitec was directly proportional to the population density of B. fibrisolvens under the supplementation of kikuyu grass with cassava flour and biomass.…”

Section: Discussionunclassified

“…Aunque durante este estudio no se realizó una cuantificación directa de la producción del CLA (debido a la utilización del sistema de simulación ruminal in vitro) en carne y productos lácteos, fue posible observar que la concentración de ácido vaccénico en Rusitec, fue directamente proporcional a la densidad poblacional de B. fibrisolvens conforme a la suplementación del pasto kikuyo con harina dairy products, it was possible to observe that the vaccenic acid concentration in Rusitec was directly proportional to the population density of B. fibrisolvens under the supplementation of kikuyu grass with cassava flour and biomass. These results suggest that the application of this type of supplementation in the field may cause a significant increase in the CLA concentration in milk and meat from bovines, if the role of vaccenic acid as one of its main originators is considered (6,24).…”

Section: Discussionunclassified

“…Its concentration is directly related with the diet supplied to animals, which is rich in linoleic, alphalinoleic acid, protein and fiber and requires active participation of the ruminal microorganisms in charge of such process, as it is the case for ruminal bacteria B. fibrisolvens (6,7,13,24). Although during this study a direct quantification of CLA production was not carried out, due to the use of a ruminal simulation system in vitro, instead of meat and influenciar la población de bacterias celulolíticas y B. fibrisolvens observada en este estudio en T2, incluyendo la suplementación de pasto de alta calidad con biomasa y harina de yuca.…”

Section: Discussionmentioning

confidence: 99%

“…Mrazek et al (23), identified that the Pseudobutyrivibrio ruminis and Butyrivibrio proteoclasticum isolations found in bovine rumen, produce succinate and propanoate, but they do not produce butyric and acetate, in contrast to the type strain for B. fibrisolvens, which produces butyric and lactic acids and formic and succinic acids at a lower quantity. The species of this genus belong to the cluster XIVa of the Subphylum Clostridium and take part in important functions of the ruminal ecosystem, including proteolysis process for fiber degradation and fatty acids biohydrogenation (13,21,24). They also have the ability to hydrogenate linoleic to vaccenic acid, through enzymatic activity, linoleic acid isomerase and linoleic acid reductase, conferring specificity to the use of linoleic acid for conjugated linoleic acid formation (6).…”

Section: Discussionmentioning

confidence: 99%

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Cellulolytic and Butyrivibrio fibrisolvens bacteria population density, after supplementing fodder diets (Pennisetum clandestinum)

Giraldo

et al. 2015

Rev MVZ Córdoba

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Objective. Determine the population density of cellulolytic bacteria, Butyrivibrio fibrisolvens and the concentration of vaccenic acid, by supplementing diets consisting of kikuyu grass (Pennisetum clandestinum Hoechst. Ex Chiov.) as base ingredient, together with cassava flour and biomass (effluent from ethanol production) in rumen simulator-Rusitec. Materials and methods. Four treatments (T) were evaluated, these were composed as: T1/Control 1: 100% kikuyu grass with a total protein intake of 23.9%, T2: a mixture of 70% kikuyu grass, 20% biomass and 10% cassava flour with a total protein intake of 19.4%; T3/Control 2: 100% kikuyu grass, with a 17.8% protein intake and T4: 70% kikuyu grass, 20% biomass and 10% cassava flour with a 15.3% protein intake. One and two-way variance analysis was made and the Pearson correlation coefficient was determined.Results. An increase was observed in the population density of viable cellulolytic bacteria (CFU/ml) and B. fibrisolvens statistically significant (p<0.005) with treatment T2, in contrast to T1, T3 and T4 treatments. In addition, there was a significant increase in the concentration of vaccenic acid (mg/L) in the ruminal content in Rusitec with the same treatment (T2). Conclusions. Results obtained in this ruminal simulation study are evidence to the benefits of kikuyu grass together with cassava flour and biomass diet implementation on the growth of ruminal cellulolytic and B. fibrisolvens bacteria, as well as on the production of vaccenic acid. The study also suggests the nutritional potential that such supplements could provide to grazing bovine feeding. RESUMENObjetivo. Determinar la densidad poblacional de bacterias celulolíticas, Butyrivibrio fibrisolvens y la concentración de ácido vaccénico, al suplementar dietas forrajeras de pasto kikuyo con harina de yuca y biomasa (resultante de la producción de etanol), en el simulador de rumen-Rusitec. Materiales 4948 REVISTA MVZ CÓRDOBA • Volumen 20(Supl) Diciembre 2015 y métodos. Se evaluaron cuatro tratamientos (T): T1/Control 1: 100% pasto kikuyo con un aporte total de 23.9% de proteína, T2: 70% pasto kikuyo, 20% biomasa y 10% harina de yuca con un aporte total de 19.4% proteína, T3/Control 2: 100% pasto kikuyo con un aporte total de 17.8% de proteína y T4: 70% pasto kikuyo, 20% biomasa y 10% harina de yuca con un aporte total de 15.3% de proteína. Se realizó un análisis de varianza (ANOVA) de una y dos vías y se determinó el coeficiente de correlación de Pearson. Resultados. Se observó un incremento en la densidad poblacional de bacterias celulolíticas viables (UFC/ml) y de B. fibrisolvens estadísticamente significativas (p<0.005) con el tratamiento T2, en comparación con los tratamientos T1, T3 y T4. Adicionalmente, se detectó un aumento significativo en la concentración de ácido vaccénico (mg/L) en el contenido ruminal del Rusitec con el mismo tratamiento (T2). Conclusiones. Los resultados obtenidos en este estudio de simulación ruminal indican que la suplementación del pasto kikuyo con harina de yuca y ...

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Section: Discussionunclassified

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Cellulolytic and Butyrivibrio fibrisolvens bacteria population density, after supplementing fodder diets (Pennisetum clandestinum)

Giraldo

et al. 2015

Rev MVZ Córdoba

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Graded replacement of maize grain with molassed sugar beet pulp modulated ruminal microbial community and fermentation profile in vitro

et al. 2017

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Influence of reducing starch in the diets with similar protein and energy contents on lactation performance, ruminal fermentation, digestibility, behaviour and blood metabolites in primiparous and multiparous dairy cows

Akhlaghi

Ghasemi

Alikhani

et al. 2022

Veterinary Medicine & Sci

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Background It is not clearly known whether parity can affect the outcomes of starch reduction in the diet of lactating dairy cows. Introduction A 2 × 2 factorial study was conducted to evaluate the effects of reducing starch in the diets with similar protein and energy contents on lactation performance, ruminal fermentation, nutrient digestibility, behaviour and blood metabolites in primiparous (PP) and multiparous (MP) dairy cows. Methods Twenty PP cows (DIM = 37 ± 10; 40 ± 5 kg/day of milk; mean ± SD) and 20 MP cows (DIM = 37 ± 9; 48 ± 5 kg/day of milk) were used in present study. Treatments were a factorial arrangement of two levels of starch (high vs. low) and two parity categories (PP vs. MP): (1) high‐starch diet (29.2% ± 0.70) and PP cows (HS‐PP); (2) low‐starch diet (22.3% ± 0.52) and PP cows (LS‐PP); (3) high‐starch diet and MP cows (HS‐MP) and (4) low‐starch diet and MP cows (LS‐MP). All diets were formulated to be similar in crude protein (16.1 % of dry matter) and NEL (1.60 Mcal/kg of dry matter) contents. The amount of metabolise protein was 2688 g/day in high‐starch diet and 2728 g/day in low‐starch diet. The experiment was conducted over two consecutive periods and included 4 weeks for adaptation and 3 weeks for data collection. Results Dry matter intake and the yield of milk true protein and lactose increased but milk fat: protein ratio and nutrient digestibility decreased for cows fed the HS diets compared with the LS diets. The ruminal proportion of propionate was greater but acetate, the acetate to propionate ratio and sorting against long particles (19 and 8 mm) were lower for cows fed the HS diets than the LS diets. Multiparous cows had a greater nutrient intake and milk yield, longer rumination meal length, greater BW, but lower plasma total antioxidant capacity, non‐esterified fatty acids, faeces pH compared with PP cows. An interaction between parity and the dietary level of starch was detected on feed efficiency measured as FCM yield/DMI in the way that only within PP cows low‐starch diet was more efficient than HS diets. We found another interaction effect of parity × starch on back fat thickens (BFT) change in the way that only within PP cows BFT change was greater for HS compared with LS diet. Conclusion Overall, regardless of the benefit derived from feeding a reduced‐starch diet by partially replacing grains with sugar beet pulp in the diets on nutrient digestibility, a reduced‐starch diet may be used more efficiently in PP than in MP cows but at expense of body reserves (i.e. BFT) loses.

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Effects of replacing dietary starch with neutral detergent–soluble fibre on ruminal fermentation, microbial synthesis and populations of ruminal cellulolytic bacteria using the rumen simulation technique (RUSITEC)

Cited by 32 publications

References 38 publications

Cellulolytic and Butyrivibrio fibrisolvens bacteria population density, after supplementing fodder diets (Pennisetum clandestinum)

Cellulolytic and Butyrivibrio fibrisolvens bacteria population density, after supplementing fodder diets (Pennisetum clandestinum)

Graded replacement of maize grain with molassed sugar beet pulp modulated ruminal microbial community and fermentation profile in vitro

Influence of reducing starch in the diets with similar protein and energy contents on lactation performance, ruminal fermentation, digestibility, behaviour and blood metabolites in primiparous and multiparous dairy cows

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