Effects of resveratrol on bone‐healing capacity in the mouse tooth extraction socket

Abstract

Background and Objective After tooth extraction, the extraction socket undergoes several steps of soft and hard tissue healing. The healing process of the extraction socket is modulated by a range of signaling factors and biochemical agents. It has been reported that resveratrol, a polyphenolic compound, exhibits various biological effects, including anti‐inflammatory, anti‐carcinogenic, antioxidant, and anti‐aging effects, and protects cardiovascular and bone tissues. In this study, we examined the cellular effects of resveratrol on human periodontal ligament (hPDL) cells and osteoblast‐like (MC3T3‐E1) cells and evaluated the bone‐healing capacity of tooth extraction sockets in mice. Material and Methods Resveratrol was applied to hPDL and MC3T3‐E1 cells to detect cell proliferation and alkaline phosphatase (ALP) activity, and qPCR was employed to understand the gene expression level in vitro. For in vivo experiment, six‐week‐old C57BL/6 male mice were randomly divided into control (n = 15) and experimental (n = 15) groups and maxillary first molars were extracted by surgery. Experimental groups received 50‐µM resveratrol on extraction sockets and analyzed the degree of new bone formation. Results Treatment of hPDL and MC3T3‐E1 cells with resveratrol increased the cell proliferation and ALP activity and enhanced the expression of ALP, BMP‐2, BMP‐4, and OC genes. Resveratrol enhanced new bone formation in the lingual extraction socket in mice. Conclusion These results suggest that resveratrol increases the cellular physiology of PDL and osteoblast including their proliferation and differentiation and may play an important role in bone‐healing capacity after tooth extraction.

Resveratrol enhances bone formation by modulating inflammation in the mouse periodontitis model

“…The final concentration of resveratrol in the mixture was maintained at 50 μM. 30 To deliver the drug into the extraction socket, we used a gas-tight Hamilton syringe 84877 (Lot No. 541628, Hamilton Co.), after which fibrin sealant (Tisseel; Cat.…”

“…The effect of resveratrol on bone formation following traumatic injury has been evaluated 30 ; however, local delivery of resveratrol after periodontitis induction has not been examined. In this study, we used the local delivery method instead of systemic administration and examined the level of bone formation after tooth extraction followed by periodontitis induction.…”

Accelerating Socket Repair via WNT3A Curtails Alveolar Ridge Resorption

“…The first objective of our study was to determine the extent to which a rodent model mimics the dimensional changes that occur in the alveolar ridge after tooth extraction (Amler et al 1960;Amler 1969;Johnson 1969). The clinical literature clearly shows that socket healing and ridge resorption are related, but precisely how the bony filling of an extraction socket curtails erosion of the alveolar ridge is not fully understood (Lekovic et al 1997;Lekovic et al 1998;Min et al 2020;reviewed by MacBeth et al 2017). In part, this is due to the fact that most clinical studies simultaneously report alveolar ridge changes and the extent of extraction socket healing (Camargo et al 2000;Pelegrine et al 2010).…”

Bioactive natural compounds as potential medications for osteogenic effects in a molecular docking approach

“…Over recent years, the active ingredients extracted from medicinal herbs involved in traditional Chinese medicine have been studied for the potential application in osteogenic effects. The compounds include naringin ( Zhao et al, 2022 ), curcumin ( Sarkar and Bose, 2019 ), quercetin ( Ren et al, 2022 ), berberine ( Jia et al, 2019 ), resveratrol ( Min et al, 2020 ), and salvianolic acids ( Ji et al, 2019 ), which boost bone regeneration by promoting bone formation or inhibiting bone resorption.…”