Effects of Retinoic Acid on Proliferation and Differentiation of HepG2 Cells

Abstract

Hepatoma cell lines have characteristics derived from both their hepatic lineage and their transformation. We examined the potential of retinoic acid to enhance the function of a hepatoma cell line, HepG2, under conditions of reduced serum as are desired for bioreactor cultivation. We found that retinoic acid drastically slows the growth of HepG2 cells and induces a more spread morphology. Retinoic acid also augments the differentiated function of the cells, as measured by albumin and urea secretion rates. Expression levels of a panel of liver-enriched transcription factor were increased from retinoic acid exposure. Overall, we demonstrate that retinoic acid has significant effects on HepG2 growth and differentiated function. These results have implications for the use of retinoic acid both as a chemotherapeutic agent and as a medium component for cells of hepatic origin.

The Anti-Proliferative Effect of Hesperidin on Hepatocarcinoma Cells HepG2

“…HCC cells are useful in understet aling both phenotypes of liver cancer et al phenotypes of hepatocytes relevant to healthy et al diseased states. Among human HCC cell lines, HepG2 are probably the best characterized (Burley & Roth, 2007). HepG2 cells serve a variety of functions in the laboratory, including assessment of the toxicology of compounds, such as antiviral drugs, genotoxicants et al antigenotoxic agents (Hongo et al, 2005).…”