Effects of salinity on embryos and larvae of tawny puffer Takifugu flavidus

Effects of water salinity (3.0-9.0 PSU) on the duration and outcome of embryogenesis in the lesser sandeel (Ammodytes tobianus (L.) as well as on survival, size, and malformation rate of the newly hatched larvae were followed, based on observations involving equipment consisting of a microscope and still and digital cameras. The images obtained and measurements of egg and larval dimensions were analyzed using the NIS elements Br. computer software package. The lowest salinity tested (3.0 PSU), similar to that in the inshore Baltic Sea waters native for the species studied, produced a lower fertilization rate and resulted in extended embryogenesis, reduced embryo survival, changes in the hatch size, and increased larval malformation rate. The high-end salinity (7.5-9.0 PSU), higher than that prevailing in the species's spawning grounds in the Baltic Sea, resulted -similarly to the low-end salinity -in disturbed development and growth of the embryos.

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“…Those findings are similar to effects reported by numerous workers studying other marine fish species [4,[8][9][10]12,34].…”

Section: Discussionsupporting

confidence: 81%

Water salinity effects on embryogenesis of the lesser sandeel, Ammodytes tobianus (Linnaeus, 1758)

et al. 2014

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“…The edible history of tiger puffer was very long in Japan, which is why it was also called “Japanese puffer”. On the contrary, the habitat range of tawny puffer is much narrower, which is only distributed in the East China Sea, the Yellow Sea and the Bohai Bay [3]. Tawny puffer maintains a smaller body size (max total length is 40 cm) and slower growth rate (reaching an average weight of 150 g after 18-month growth) than tiger puffer.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome Analysis of Artificial Hybrid Pufferfish Jiyan-1 and Its Parental Species: Implications for Pufferfish Heterosis

et al. 2013

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Jiyan-1 puffer, the F1 hybrid of Takifugu rubripes and Takifugu flavidus, displays obvious heterosis in the growth performance, flavor and stress tolerance. In the present study, comparative analysis for the transcriptomes of T. rubripes, T. flavidus and Jiyan-1 was performed aiming to reveal the possible mechanisms of heterosis in pufferfish. Whole transcriptomes were sequenced using the SOLiD4 platform, and a total of 44,305 transcripts corresponding to 18,164 genes were identified collectively. A total of 14,148 transcripts were differentially expressed. By comparing the gene expression patterns of the three samples, the coexistence of overdominance, dominance, underdominance and additivity was observed in the gene action modes of Jiyan-1. There were 2,237 transcripts in the intersection of the differentially expressed transcripts from Jiyan-1 versus T. rubripes and Jiyan-1 versus T. flavidus, among which 213 transcripts were also in the T. rubripes versus T. flavidus. The potential functions of the remaining 2,024 transcripts were mainly associated with metabolic process, nucleotide binding and catalytic activity. The enrichment results indicated metabolism was the most activated biological function in the heterosis. In addition, 35 KEGG pathways were retrieved as affiliated with more than three differentially expressed transcripts and 8,579 potentially novel transcript isoforms were identified for Jiyan-1. The present study revealed the coexistence of multiple gene actions in the hybrid puffer, indicated the importance of metabolism, ion binding function and kinase activity, as well as provided a list of candidate genes and pathways for heterosis. It could be helpful for the better understanding of the determination and regulation mechanisms of heterosis.

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“…Comparativamente, la mayoría de los embriones de peces de agua dulce son estenohalinos (Gbulubo & Erondu, 1998;Ekmekçi, 2002;FashinaBombata & Busari, 2003;Albert et al, 2004;Bhattacharya et al, 2006), no superando generalmente el umbral de salinidad de 10 por breves períodos de exposición. En cambio los peces eurihalinos soportan amplios gradientes salinos (Mihelakakis & Kitajima, 1994;Hart & Purser, 1995;Smith et al, 1999;Berlinsky et al, 2004;Yang & Chen, 2006;Zhang et al, 2010;Brown et al, 2011) por períodos prolongados de exposición. Una diferencia observada en el embrión de G. maculatus respecto de otros embriones de peces eurihalinos fue su capacidad para completar la ontogenia embrionaria en agua dulce, sin que la ausencia de sal influyera en los porcentajes de supervivencia embrionaria (entre los tratamientos a salinidades de 0, 4, 8, 12, 16 y 20 no hubo diferencias significativas).…”

Section: Discussionunclassified

Efecto de la salinidad en la supervivencia embrionaria de puye Galaxias maculatus (Jenyns, 1842)

Barile¹,

Escudero²,

Carreño³

et al. 2015

lajar

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RESUMEN.Se evaluó e l efecto de la salinidad en la supervivencia embrionaria, período embrionario y período de eclosión de puye, Galaxias maculatus. Se utilizaron ocho tratamientos, con salinidades de 0, 4, 8, 12, 16 y 20 obteniéndose supervivencias embrionarias promedios respectivas de 84,9; 84,0; 85,5; 86,1; 82,6 y 75,8%, sin diferencias significativas entre ellas, mientras que con salinidad de 24 se obtuvo sobrevivencia de 10,4%, significativamente diferente al resto de los tratamientos y con salinidad de 28 causó 100% de mortalidad. A diferencia del estado juvenil y adulto, el embrión es incapaz de resistir la salinidad marina y su umbral de tolerancia se encuentra entre salinidades de 24 y 28. Las mayores supervivencias promedios larvales a 10 días de vida fueron con salinidades intermedias de 8, 12 y 16 de 79,3; 80,3 y 74,6% respectivamente, sin diferencias significativas entre ellas, demostrando alta viabilidad del embrión post-eclosión. Los períodos embrionarios promedios (50%) más breves fueron a bajas salinidades 0, 4, 8, 12 con 28,7; 28,0; 29,7 y 29,7 días y los más extensos en los tratamientos con salinidades de 16 y 20, con 34,0 y 34,3 días, sin diferencias significativas entre ellos. Los períodos de eclosión promedio (50%) con salinidades de 0, 4, 8, 12, 16, 20 y 24 fueron de 5,0; 5,5; 7,5; 8,8; 13,0; 14,3 y 15,8 días respectivamente, sin diferencias significativas entre ellos. Palabras clave: Galaxias maculatus, puye, eclosión, incubación, tolerancia salina, acuicultura. Effect of salinity on survival of embryos of jollytailGalaxias maculatus (Jenyns, 1842)ABSTRACT. Eight different salinity effects on embryonic survival and embryonic and hatching phase time duration of jollytail (Galaxias maculatus) were assessed. Mean survival rates were higher at salinities values of 0, 4, 8, 12, 16 and 20 with 84.9, 84.0, 85.5, 86.1, 82.6 and 75.8% respectively with no significant differences between them; in contrast to salinity of 24 was obtained by 10.4%, significantly different from other treatments. Salinity of 28 caused 100% of mortality. According to results, the embryo is unable to resist ocean salinity and its tolerance limit is between 24 and 28. The highest larval survivals at 10 days after hatching occurred in those treatments in which embryos were performed to intermediate salinities of 8, 12 and 16 with 79.3, 80.3 and 74.6% respectively, with no significant differences between them. These results demonstrate high post-hatching viability. Embryonic time period (50%) were shorter at low salinities 0, 4, 8 and 12 with 28.7, 28.0, 29.7 and 29.7 days and the largest, in the treatments of salinities of 16 and 20 with 34.0 and 34.3 days. No significant differences were found between all of them. Regarding the average hatching time periods (50%) in the treatments of salinities of 0, 4, 8, 12, 16, 20 and 24 were 5.0, 5.5, 7.5, 8.8, 13, 0, 14.3 and 15.8 days respectively, without significant differences between them.

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Effects of salinity on embryos and larvae of tawny puffer Takifugu flavidus

Cited by 41 publications

References 25 publications

Water salinity effects on embryogenesis of the lesser sandeel, Ammodytes tobianus (Linnaeus, 1758)

Water salinity effects on embryogenesis of the lesser sandeel, Ammodytes tobianus (Linnaeus, 1758)

Transcriptome Analysis of Artificial Hybrid Pufferfish Jiyan-1 and Its Parental Species: Implications for Pufferfish Heterosis

Efecto de la salinidad en la supervivencia embrionaria de puye Galaxias maculatus (Jenyns, 1842)

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