Tawny pu¡er Takifugu £avidus is a species found in China considered to have potential for aquaculture. Experiments were conducted to determine the optimal temperature for its incubation and larval culture. Fertilized eggs collected from cultured broodstocks that were induced to ovulate with a [D-Ala 6 -Pro 9 -Net]-luteinizing hormone-releasing hormone analogue were inseminated. The e¡ect of temperature (19, 20, 23, 26 and 29 1C) on the hatch rate, incubation period, viability of 24 h post-hatch larvae and total mortality rate was assessed. The effect of temperature (20, 23, 26 and 29 1C) on the growth and survival of larvae from 3 to 19 days after hatching (dah) was also assessed. The results showed that the optimal temperature for successful development of fertilized eggs ranged from 23 to 26 1C, and the highest hatch rate, the optimal viability of 24 h post-hatch larvae and the lowest total mortality rate were all predicted using quadratic equations. The relationship between temperature and the incubation period of tawny pu¡er eggs was determined using the e¡ective degree-day model. The temperature at developmental zero (t 0 ) was 11.34 1C, and the sum of e¡ective degree-days (k) was 52.356. The survival rate of tawny pu¡er larvae at 20 1C was signi¢cantly lower than among 23, 26 and 29 1C, whereas the survival rate was not signi¢cantly di¡erent from that at 23, 26 and 29 1C. The larval growth rate increased rapidly as the temperature increased, showing a linear relationship in the range of temperatures investigated. The optimal temperature for larval culture ranged from 23 to 29 1C.
The respiratory rates of Tawny puffer Takifugu flavidus juvenile were measured at four temperatures (20, 23, 26 and 29 °C) and seven salinities (5, 10, 15, 20, 25, 30 and 35 g L−1). The results showed that both temperature and salinity significantly affected the oxygen consumption of tawny puffer juvenile. The oxygen consumption rate (OCR) increased significantly with an increase in the temperature from 20 to 29 °C. Over the entire experimental temperature range (20–29 °C), the Q10 value was 1.59, and the lowest Q10 value was found between 23 and 26 °C. The optimal temperature for the juvenile lies between 23 °C and 26 °C. The OCR at 25 g L−1 was the highest among all salinity treatments. The OCRs show a parabolic relationship with salinity (5–35 g L−1). From the quadratic relationship, the highest OCR was predicted to occur at 23.56 g L−1. The optimal salinity range for the juvenile is from 23 to 25 g L−1. The results of this study are useful towards facilitating an increase in the production of the species juvenile culture.
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