Effects of Salvia miltiorrhiza extract with supplemental liquefied calcium on osteoporosis in calcium-deficient ovariectomized mice

Park, Bong‐Kyun; Song, Hae Seong; Kwon, Jeong Eun; Cho, Se Min; Jang, Seon‐A; Kim, Mi Yeon; Kang, Se Chan

doi:10.1186/s12906-017-2047-y

Cited by 22 publications

(8 citation statements)

References 69 publications

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“…Studies have shown increased serum levels of ALP in OVX rats (Park et al. 2017 ); in our study, FAEE treatment tended to reduce ALP levels in serum. Those data indicate that FAEE can inhibit the bone turnover in OVX rats.…”

Section: Discussionsupporting

confidence: 64%

FAEE exerts a protective effect against osteoporosis by regulating the MAPK signalling pathway

Wang

Fan

et al. 2022

Pharmaceutical Biology

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Context Ferulic acid ethyl ester (FAEE) is abundant in Ligusticum chuanxiong Hort. (Apiaceae) and grains, and possesses diverse biological activities; but the effects of FAEE on osteoporosis has not been reported. Objective This study investigated whether FAEE can attenuate osteoclastogenesis and relieve ovariectomy-induced osteoporosis via attenuating mitogen-activated protein kinase (MAPK). Materials and methods We stimulated RAW 264.7 cells with receptor activator of NF-κB ligand (RANKL) followed by FAEE. The roles of FAEE in osteoclast production and osteogenic resorption of mature osteoclasts were evaluated by tartrate resistant acid phosphatase (TRAP) staining, expression of osteoclast-specific genes, proteins and MAPK. Ovariectomized (OVX) female Sprague-Dawley rats were administered FAEE (20 mg/kg/day) for 12 weeks to explore its potential in vivo , and then histology was undertaken in combination with cytokines analyses. Results FAEE suppressed RANKL-induced osteoclast formation (96 ± 0.88 vs. 15 ± 1.68) by suppressing the expression of osteoclast-specific genes, proteins and MAPK signalling pathway related proteins (p-ERK/ERK, p-JNK/JNK and p-P38/P38) in vitro . In addition, OVX rats exposed to FAEE maintained their normal calcium (Ca) (2.72 ± 0.02 vs. 2.63 ± 0.03, p < 0.05) balance, increased oestradiol levels (498.3 ± 9.43 vs. 398.7 ± 22.06, p < 0.05), simultaneously reduced levels of bone mineral density (BMD) (0.159 ± 0.0016 vs. 0.153 ± 0.0025, p < 0.05) and bone mineral content (BMC) (0.8 ± 0.0158 vs. 0.68 ± 0.0291, p < 0.01). Discussion and conclusions These findings suggested that FAEE could be used to ameliorate osteoporosis by the MAPK signalling pathway, suggesting that FAEE could be a potential therapeutic candidate for osteoporosis.

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Section: Discussionsupporting

confidence: 64%

FAEE exerts a protective effect against osteoporosis by regulating the MAPK signalling pathway

Wang

Fan

et al. 2022

Pharmaceutical Biology

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“…For our in vivo study, OVX-induced osteoporosis in mice was selected as a bone repair model because it has been widely used to evaluate the bone formation of bioactive molecules and biomaterials in bones (41, 42). After treatment with AG for 4 weeks, bone histomorphometric analysis and micro-CT analysis were used to measure the effect of AG on bone formation.…”

Section: Discussionmentioning

confidence: 99%

Astragalin Promotes Osteoblastic Differentiation in MC3T3-E1 Cells and Bone Formation in vivo

et al. 2019

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Astragalin (AG) is a biologically active flavonoid compound that can be extracted from a number of medicinal plants. However, the effects of AG on osteoblastic differentiation in mouse MC3T3-E1 cells and on bone formation in vivo have not been studied fully. In this study, we found that the activities of alkaline phosphatase (ALP) and mineralized nodules in MC3T3-E1 cells were both significantly increased after treatment with AG (5, 10, and 20 μM). Meanwhile, the mRNA and protein levels of osteoblastic marker genes in MC3T3-E1 cells after AG treatment were markedly increased compared with a control group. In addition, the levels of BMP-2, p-Smad1/5/9, and Runx2 were significantly elevated in AG-treated MC3T3-E1 cells. Moreover, we found that the protein levels of Erk1/2, p-Erk1/2, p38, p-p38, and p-JNK were also significantly increased in AG-treated MC3T3-E1 cells compared to those in the control group. Finally, in vivo experiments demonstrated that AG significantly promoted bone formation in an ovariectomized (OVX)-induced osteoporotic mouse model. This was evidenced by significant increases in the values of osteoblast-related parameters (BFR/BS, MAR, Ob.S/BS, and Ob.N/B.Pm) and bone histomorphometric parameters (BMD, BV/TV, Tb.Th, and Tb.N.) in OVX mice after AG treatment (5, 10, and 20 mg/kg). Collectively, these results demonstrated that AG may promote osteoblastic differentiation in MC3T3-E1 cells via the activation of the BMP and MAPK pathways and promote bone formation in vivo . These novel findings indicated that AG may be a useful bone anabolic agent for the prevention and treatment of osteoporosis.

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“…However, Nich et al () showed that OPG mRNA expression was not significantly different between OVX mice and OVX mice treated with E2 groups. Park et al () reported that Salvia miltiorrhiza extract increased the serum OPG levels without affecting serum E2 levels. Therefore, we suggest the mechanism responsible for the anti‐osteoporotic effects of PCE17 observed in OVX mice is partly different from that of estrogen.…”

Section: Discussionmentioning

confidence: 99%

PCE17 and its active compounds exert an anti-osteoporotic effect through the regulation of receptor activator of nuclear factor-κB ligand in ovariectomized mice

Jeong

Kim

et al. 2018

J Food Biochem

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PCE17 has been developed to prevent and treat osteoporosis in Korea. Here, we investigated the anti‐osteoporotic effects of PCE17 and its active compounds, a mixture of tectoridin, tectorigenin, and tectorigenin 7‐O‐xylosylglucoside (Tec) and hesperidin, in ovariectomized (OVX) mice. Serum levels of RANKL, TRAP, and Ntx1 were higher in OVX‐control mice than normal controls. However, these increased levels were decreased by PCE17, Tec, or hesperidin. In addition, serum levels of alkaline phosphatase, osteoprotegerin, and osteocalcin were higher in PCE17, Tec, or hesperidin‐fed mice than in OVX controls. PCE17 or hesperidin increased bone mineral density, trabecular number, and connectivity density, and decreased total porosity. Furthermore, no obvious hypercholesterolemia or liver toxicity developed in OVX‐treated mice at PCE17 dosages up to 460 mg/kg. In conclusion, PCE17 has an anti‐osteoporotic effect in OVX mice. These results suggest that PCE17 has potential use as an alternative therapeutic agent for the prevention and treatment of osteoporosis. Practical applications PCE17 has been developed to prevent osteoporosis as a health functional food. PCE17 has an anti‐osteoporotic effect through the maintenance bone homeostasis by redressing the balance between bone resorption and bone formation, and that PCE17 be considered potentially useful preventative or protective treatment for postmenopausal osteoporosis.

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Effects of Salvia miltiorrhiza extract with supplemental liquefied calcium on osteoporosis in calcium-deficient ovariectomized mice

Cited by 22 publications

References 69 publications

FAEE exerts a protective effect against osteoporosis by regulating the MAPK signalling pathway

FAEE exerts a protective effect against osteoporosis by regulating the MAPK signalling pathway

Astragalin Promotes Osteoblastic Differentiation in MC3T3-E1 Cells and Bone Formation in vivo

PCE17 and its active compounds exert an anti-osteoporotic effect through the regulation of receptor activator of nuclear factor-κB ligand in ovariectomized mice

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