Effects of Salvia miltiorrhiza injection on apoptosis of Schwann cells induced by hydrogen peroxide

Abstract

Background: Salvia miltiorrhiza is a traditional Chinese medicine with remarkable antioxidant, antibacterial, and anticoagulant properties. In the present study, we investigated the effects of Salvia miltiorrhiza injection in protecting Schwann cells (SCs) from hydrogen peroxide (H 2 O 2 )-induced cell apoptosis.Methods: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and immunofluorescence staining were used to detect the establishment of the SC apoptosis model induced by H 2 O 2 . The effect of Salvia miltiorrhiza injection on injured cell morphology was observed, and the effect on cell apoptosis was determined by Annexin V-fluorescein isothiocyanate (FITC) apoptosis detection kit and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay. Western blotting analysis was used to detect the effect of Salvia miltiorrhiza injection on apoptosis-related protein expression. Results:The results of the MTT assay showed that cell activity significantly decreased after treatment with 1 mM H 2 O 2 , but different concentrations of Salvia miltiorrhiza injection could improve cell activity at different degrees. The number of cells increased significantly after treatment with Salvia miltiorrhiza injection. Annexin V-FITC/PI double staining and TUNEL results revealed that Salvia miltiorrhiza injection could significantly reduce apoptosis induced by H 2 O 2 . Western blotting analysis showed that the expression of Bcl-2 was significantly upregulated, while the expression level of Bax was significantly downregulated.Conclusions: Salvia miltiorrhiza injection can protect SCs from H 2 O 2 -induced cell apoptosis, and has potential therapeutic effects in neurological disease.

Effects of Moleac 901 after severe spinal cord injury on chronic phase in Wistar rats

“… MLC901 component Active compounds Effect Mechanism Astragali radix (Astragalus membrana-ceus) (AR) [ 45 , 46 ] Isoflavonoid Apoptosis, glia reactivity, inflammatory, neuroprotective, antioxidant Activation of the P13K/AKT pathway, MKP-1 activation simultaneously weakens P38 and ERK, decreases AQP4, TNF-α, inhibits the NF-κB pathway, decreased SOD, MDA and GFAP expression. Radix salviae miltorrhizae (RSM) [ 39 , 47 , 48 ] Salvianolic acid Inflammation, edema, oxidative stress Inhibits ERK, inhibits the NF-κB path, decreases MDA expression. Radix paeonieae rubra (RPR) [ 39 , 40 , [47] , [48] , [49] ] Paeoniflorin Apoptosis, inflammation Reducing Caspase-3, lowering TLR mediated signals.…”

“… Radix salviae miltorrhizae (RSM) [ 39 , 47 , 48 ] Salvianolic acid Inflammation, edema, oxidative stress Inhibits ERK, inhibits the NF-κB path, decreases MDA expression. Radix paeonieae rubra (RPR) [ 39 , 40 , [47] , [48] , [49] ] Paeoniflorin Apoptosis, inflammation Reducing Caspase-3, lowering TLR mediated signals. Rhizoma chuanxiong (RC) [ 39 , 42 ] Alkaloid Tetra-methylpyrazine , Ferulic acid, senkyunolide I, dan ligustilide Apoptosis, inflammation Increases BCL-2, decreases Caspase-3, activation of the P13K/AKT pathway, interacting with the ALB, AKT1, MAPK1, MMP9, and EGFR genes decreases proinflammatory cytokines (IL-1mit and TNF-α) and increases IL-10).…”

Effects and molecular mechanisms of Achyranthes bidentata polypeptide k on proliferation of Schwann cells

“…20190225-004]. The culture method used has been previously described (14). In brief, cells were cultured in DMEM medium (Corning, USA) supplemented with 10% of fetal bovine serum (FBS; Gibco, Grand Island, NY, USA), then anti-Thy1.1 antibody (Sigma, St. Louis, MO, USA) and rabbit complement (Invitrogen, Carlsbad, CA, USA) were added to remove fibroblasts.…”