Effects of salvianolic acid B on L-type calcium channels and myocardial contractility in isolated rat ventricular myocytes and hERG K+ channels expressed in HEK293 cells

Abstract

Salvianolic acid B (Sal B), one of the chief water-soluble constituents in Radix Salviae Milthiorrhizae, has often been reported to possess considerable cardiovascular regulatory effects. However, the underlying biochemical and cellular mechanisms of its cardioprotection remain unclear. This study was designed to evaluate the role of Sal B regulation in L-type Ca channel currents (I) and cell contractility in rat cardiomyocytes and hERG K channels expressed in HEK293 cells with the patch-clamp and Ca imaging techniques to clarify its underlying cardioprotective mechanisms. Exposure to Sal B blocked I with IC of 2.07 × 10 M, shifted the curves of current and voltage upwards, shifted the curves of activation and inactivation to the left, and significantly inhibited the amplitude of the cell shortening, but the regulatory effects of Sal B on the expressed rapidly activating delayed rectifier potassium current (I) did not reach a significant level. These results indicate that the cardioprotective mechanisms of Sal B may be related to the attenuation of calcium overload by directly inhibiting I and consequently decreasing myocardial contractility without causing drug-induced long QT syndrome (LQTS).

Protective effects of salvianolic acid B against hydrogen peroxide‑induced apoptosis of human umbilical vein endothelial cells and underlying mechanisms

“…Therefore, the present study further examined the anti-atherosclerotic effects of Sal B and investigated the underlying mechanisms. Previous studies have confirmed that Sal B has anti-oxidative effects and eliminates superoxide anion radicals (O 2− ), thereby suppressing hydrogen peroxide (H 2 O 2 )-induced apoptosis (10,11). Another study indicated that Sal B promotes human umbilical vein endothelial cell (HUVEC) proliferation (12).…”

Salvianolic Acid B Protects Intervertebral Discs from Oxidative Stress‐Induced Degeneration via Activation of the JAK2/STAT3 Signaling Pathway

“…Salvianolic acid B (SAB) is the most abundant water-soluble compound extracted from Danshen. Previous studies have confirmed that SAB has antioxidative properties and eliminates superoxide anion radicals (·O 2 − ), thereby inhibiting hydrogen peroxide- (H 2 O 2 -) induced apoptosis [ 23 , 24 ]. It has also been reported that SAB can increase the proliferation of human umbilical vein endothelial cells [ 25 ].…”

Long chain n-3 polyunsaturated fatty acid intake across the life span for cardiovascular disease prevention in women

“…An abundance of evidence points to the beneficial effects of LCn-3 PUFA on the pathological pathways that underlie CVD. The mechanisms mediating cardioprotective effects include those related to their preferential incorporation into cellular membranes, which may reduce risk of sudden cardiac death by lowering heart rate (27) , improving cardiac autonomic function (28,29) and modulating ion channel function and cell signalling pathways (30) . Other pathways involved their direct effects on vasodilators in the vasculature (31) , inhibition of inflammatory molecules and conversion to less proinflammatory eicosanoids and pro-resolving mediators that dampen and resolve inflammation (32) , also by inhibiting platelet aggregation at higher doses (33) , and by increasing lipoprotein lipase expression and activity (34) , lipid oxidation (35) and decreasing de novo lipogenesis via modulation of liver transcription factors that regulate expression of lipogenic enzymes (36,37) , leading to a reduction in blood triglycerides (38) .…”