Effects of SB202190 on expression levels of IL-6 and NF-κB in flap ischemia-reperfusion injury

Abstract

The aim of the current study was to investigate the effect of SB202190, a specific inhibitor of p38 MAPK signaling pathway, on the expression levels of IL-6 and NF-κB in flap ischemia-reperfusion injury. Healthy Sprague-Dawley rats were randomly divided into four groups of 12 each. For the ischemia-reperfusion group, the flap was constructed and then sutured after 8 h of ischemia. For the saline group, rats were intraperitoneally infused with saline at regular intervals after flap ischemia-reperfusion. For the inhibitor group, rats were intraperitoneally infused with SB202190 at regular intervals after flap ischemia-reperfusion. For the control group, the flap was constructed and then sutured immediately. The flap survival rate of each group was measured after 7 days. The concentration of IL-6 in serum was measured by ELISA kit. The mRNA and protein expression levels of IL-6 and NF-κB in the flap were measured using RT-PCR and western blot analysis, respectively. In the ischemia-reperfusion group and the saline group, the flap survival rates were much lower than that in the control group (P<0.05). By contrast, the mRNA and protein expression levels of IL-6 and NF-κB in the flap and the concentration of IL-6 in serum were much higher (P<0.05). In the inhibitor group, the flap survival rate was significantly higher than those in the ischemia-reperfusion and saline groups (P<0.05). By contrast, the concentration of IL-6 in serum and the mRNA and protein expression levels of NF-κB and IL-6 in the flap were significantly decreased (P<0.05). The results show that, SB202190 played a role in the protection of the flap by reducing the inflammatory response in flap ischemia-reperfusion injury.

Detrimental Effect of Sitagliptin Induced Autophagy on Multiterritory Perforator Flap Survival

“…This delay phenomenon eventually disappeared at 1 st POD in the SCZ which means that IR injury did happen in the potential territory. IR injury is a leading cause of necrosis after reconstructive flap surgery ( Guo et al., 2018 ). Apoptosis and oxidative stress are principal causes of cell death after reperfusion ( Burns et al., 1998 ; Tanaka et al., 2009 ).…”

Cannabidiol Improves Random-Pattern Skin Flap Survival in Rats: Involvement of Cannabinoid Type-2 Receptors

“…(1) sham group, nontreated and nonoperated animals; (2) TNF-α concentrations decreased considerably after treatment with CBD of 1 mg/kg compared with the control (p <0.05 and <0.001, respectively). Administering SR144528 reversed the favorable effects of CBD of 1 mg/kg, both macroscopically and microscopically.…”

“…Animals were divided into seven different groups as follows: ( 1 ) sham group, nontreated and nonoperated animals; ( 2 ) control group, vehicle-treated (saline + DMSO) and operated animals; ( 3 4 5 6 ) CBD groups, rats received CBD at different doses (0.3, 1, 5, or 10 mg/kg) 60 minutes prior to cutting the main artery in four separate groups; and ( 6 ) SR144528 + CBD group, rats received SR144528 (2 mg/kg), a high affinity and selective CB2 inverse agonist, 60 minutes before the most effective dose of CBD (1 mg/kg) to investigate the possible involvement of CB2 receptors.…”

“…1 Surgeons are constantly dealing with the flap tissue design to reach a flap tissue with acceptable functional and esthetic outcomes and decrease the number of donor sites. 2 The random-pattern skin flap is a prevalent surgical method that covers skin deformities, mainly caused by burn injuries, tumor removal surgeries, or congenital defects. 3,4 One of the most concerning postoperative complications of such flaps is distal part necrosis, limiting its clinical application.…”