Effects of Schizolobium parahyba Extract on Experimental Bothrops Venom-Induced Acute Kidney Injury

Martines, Monique Silva; Mendes, Mirian Machado; Shimizu, Maria Heloísa Massola; Rodrigues, Veridiana M.; Castro, Isac de; Filho, Sebastião Rodrigues Ferreira; Malheiros, Denise Maria Avancini Costa; Yu, Liang; Burdmann, Emmanuel A.

doi:10.1371/journal.pone.0086828

Cited by 8 publications

(10 citation statements)

References 40 publications

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“…Renal ischemia has been considered the hallmark of AKI following B . jararaca envenomation [ 6 , 34 , 35 ]. Accordingly, we observed severe renal vasoconstriction, associated with increased renal vascular resistance, in BJ animals at 40 and 70 minutes after venom infusion.…”

Section: Resultsmentioning

confidence: 99%

“…We administered intravenous BJ after the surgical procedure for the inulin clearance study, as previously reported by Martines et al [ 6 ]. Animals were allocated into four groups:…”

Section: Methodsmentioning

confidence: 99%

“…These accidents predominantly affect young adults, especially males, yet almost 1/3 of the cases occur in children [ 5 ]. Moreover, snake envenomation may also be seen as an occupational hazard whereas it has been associated with significant morbidity and disability mostly in agricultural workers (food producers) [ 4 – 6 ]. Thus, there is a great impact of venomous snakebites in the economy of a community or even a country.…”

Section: Introductionmentioning

confidence: 99%

“…However, there are several limitations of the antivenom therapy such as high cost, limited availability, (e.g. in remote areas), severe anaphylactic reactions, and inefficiency to treat local manifestations [ 6 , 20 – 22 ]. Therefore, the study of novel therapies in Bothrops envenomation is a fruitful area for future research.…”

Section: Introductionmentioning

confidence: 99%

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Allopurinol attenuates acute kidney injury following Bothrops jararaca envenomation

et al. 2017

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Snakebites have been recognized as a neglected public health problem in several tropical and subtropical countries. Bothrops snakebites frequently complicate with acute kidney injury (AKI) with relevant morbidity and mortality. To date, the only treatment available for Bothrops envenomation is the intravenous administration of antivenom despite its several limitations. Therefore, the study of novel therapies in Bothrops envenomation is compelling. The aim of this study was to evaluate the protective effect of Allopurinol (Allo) in an experimental model of Bothrops jararaca venom (BJ)-associated AKI. Five groups of Wistar rats were studied: Sham, Allo, BJ, BJ+Allo, BJ+ipAllo. BJ (0.25 mg/kg) was intravenously injected during 40’. Saline at same dose and infusion rate was administered to Sham and Allo groups. Allo and BJ+Allo groups received Allo (300 mg/L) in the drinking water 7 days prior to Saline or BJ infusion respectively. BJ+ipAllo rats received intraperitoneal Allo (25 mg/Kg) 40’ after BJ infusion. BJ rats showed markedly reduced glomerular filtration rate (GFR, inulin clearance) associated with intense renal vasoconstriction, hemolysis, hemoglobinuria, reduced glutathione and increased systemic and renal markers of nitro-oxidative stress (Nitrotyrosine). Allo ameliorated GFR, renal blood flow (RBF), renal vascular resistance and arterial lactate levels. In addition, Allo was associated with increased serum glutathione as well as reduced levels of plasma and renal Nitrotyrosine. Our data show that Allo attenuated BJ-associated AKI, reduced oxidative stress, improved renal hemodynamics and organ perfusion. It might represent a novel adjuvant approach for Bothrops envenomation, a new use for an old and widely available drug.

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Section: Resultsmentioning

confidence: 99%

“…We administered intravenous BJ after the surgical procedure for the inulin clearance study, as previously reported by Martines et al [ 6 ]. Animals were allocated into four groups:…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Allopurinol attenuates acute kidney injury following Bothrops jararaca envenomation

et al. 2017

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“…Acute renal failure is a common complication of Bothrops viper envenomation. High venom concentration at the renal tissue, direct action of venom components on renal tubules and renal epithelial cells, hemoglobinuria, vascular occlusion and oxidative stress are among factors incriminated in the pathogenesis of renal failure [ 12 ].…”

Section: Discussionmentioning

confidence: 99%

L-Aminoacid Oxidase from Bothrops leucurus Venom Induces Nephrotoxicity via Apoptosis and Necrosis

et al. 2015

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Acute renal failure is a common complication caused by Bothrops viper envenomation. In this study, the nefrotoxicity of a main component of B. leucurus venom called L-aminoacid oxidase (LAAO-Bl) was evaluated by using tubular epithelial cell lines MDCK and HK-2 and perfused kidney from rats. LAAO-Bl exhibited cytotoxicity, inducing apoptosis and necrosis in MDCK and HK-2 cell lines in a concentration-dependent manner. MDCK apoptosis induction was accompanied by Ca2+ release from the endoplasmic reticulum, reactive oxygen species (ROS) generation and mitochondrial dysfunction with enhanced expression of Bax protein levels. LAAO-Bl induced caspase-3 and caspase-7 activation in both cell lines. LAAO-Bl (10 μg/mL) exerts significant effects on the isolated kidney perfusion increasing perfusion pressure and urinary flow and decreasing the glomerular filtration rate and sodium, potassium and chloride tubular transport. Taken together our results suggest that LAAO-Bl is responsible for the nephrotoxicity observed in the envenomation by snakebites. Moreover, the cytotoxic of LAAO-Bl to renal epithelial cells might be responsible, at least in part, for the nephrotoxicity observed in isolated kidney.

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Acute kidney injury due to tropical infectious diseases and animal venoms: a tale of 2 continents

Burdmann

Jha

2017

Kidney International

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South and Southeast Asia and Latin American together comprise 46 countries and are home to approximately 40% of the world population. The sociopolitical and economic heterogeneity, tropical climate, and malady transitions characteristic of the region strongly influence disease behavior and health care delivery. Acute kidney injury epidemiology mirrors these inequalities. In addition to hospital-acquired acute kidney injury in tertiary care centers, these countries face a large preventable burden of community-acquired acute kidney injury secondary to tropical infectious diseases or animal venoms, affecting previously healthy young individuals. This article reviews the epidemiology, clinical picture, prevention, risk factors, and pathophysiology of acute kidney injury associated with tropical diseases (malaria, dengue, leptospirosis, scrub typhus, and yellow fever) and animal venom (snakes, bees, caterpillars, spiders, and scorpions) in tropical regions of Asia and Latin America, and discusses the potential future challenges due to emerging issues.

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Effects of Schizolobium parahyba Extract on Experimental Bothrops Venom-Induced Acute Kidney Injury

Cited by 8 publications

References 40 publications

Allopurinol attenuates acute kidney injury following Bothrops jararaca envenomation

Allopurinol attenuates acute kidney injury following Bothrops jararaca envenomation

L-Aminoacid Oxidase from Bothrops leucurus Venom Induces Nephrotoxicity via Apoptosis and Necrosis

Acute kidney injury due to tropical infectious diseases and animal venoms: a tale of 2 continents

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