Effects of selected chelating agents and metals on the stability of liver lysosomes

Progesterone-induced lysis of rat kidney lysosomes as studied by changes in light-absorbance

“…Stabilizing effect of Zn2+ on pH-induced and progesterone-induced lysis Low concentrations of Zn2+ have been reported to stabilize rat liver lysosomes against spontaneous thermal and pH-induced lysis (Chrapil et al, 1972). We suspended the primary granule fraction in 0.25M-sucrose containing 0-lOmM-ZnSO4 (ZnSO4 did not appreciably alter the pH ofthe medium) and measured the rate of absorbance decrease either on addition Vol.…”

Inhibition of Lethality in Endotoxin Challenged Mice Treated With Zinc Chloride

“…There is evidence that the zinc ion (Zn2+) can participate in the stabilization of membranes (14). Zinc is known to facilitate the isolation of intact plasma membranes (15); it can inhibit the spontaneous hemolysis of erythrocytes when given orally to rats (3); and, finally, zinc inhibits in vitro the release of enzymes from lysosomes obtained from rat liver (4,8). In addition, the zinc ion can strongly inhibit cathepsin B (10), an acid protease which can catalyze the formation of bradykinin from kininogen (1 It is significant that the degree of protection was dependent on the amount of zinc administered (Table 1).…”

Effect of heavy metal cations on the activity of cathepsin D (in vitro study)

“…The metal binding exogenous compounds include ethylenodiaminotetraacetate acid, diethylenotriaminopentaacetic acid, N-acetylocystein, penicillamine and deferoxamine [43][44][45]. The concentrations of heavy metal cations used in the current experiment resembled those in the blood and tissues of intoxicated subjects [46].…”