Effects of Selenium and Light Wavelengths on Liquid Culture of Cordyceps militaris Link

Abstract

To investigate the effects of selenium and light wavelengths on the growth of liquid-cultured Cordyceps militaris and the main active components' accumulation, culture conditions as selenium selenite concentrations and light of different wavelengths were studied. The results are: adenosine accumulation proved to be significantly selenium dependent (R(2) = 0.9403) and cordycepin contents were determined to be not significantly selenium dependent (R(2) = 0.3845) but significantly enhanced by selenium except for 20 ppm; there were significant differences in cordycepin contents, adenosine contents, and mycelium growth caused by light wavelengths: cordycepin, blue light > pink light > daylight, darkness, red light; adenosine, red light > pink light, darkness, daylight, blue light; and mycelium growth, red light > pink light, darkness, daylight > blue light. In conclusion, light wavelength had a significant influence on production of mycelia, adenosine, and cordycepin, so lightening wavelength should be changed according to target products in the liquid culture of C. militaris.

Metabolomic profile and nucleoside composition of Cordyceps nidus sp. nov. (Cordycipitaceae): A new source of active compounds

“…Curiously, we found that the presence of sodium selenite did not induce the production of adenosine and cordycepin, which is in disagreement with the promoting activity of selenium on nucleosides produced by C . militaris [15]. Selenium complexes can promote the activity of several enzymes involved in cordycepin synthesis [60].…”

“…These may be extracted from fruiting bodies or mycelia and are currently studied as therapeutic agents [7–8, 10, 12–13]. Nucleosides can be differentially induced by intrinsic or environmental factors, such as the developmental stage of the fungus, light exposure and wavelength, the composition of the culture media, among other factors [7–8], [12–15]. Traditionally, the nucleosides cordycepin (3’-deoxyadenosine), adenosine, inosine, guanosine, and uridine have been used as markers to assess the quality of natural and cultured species of Cordyceps [4, 16–17].…”

Direct Biotransformation of Dioscin into Diosgenin in Rhizome of Dioscorea zingiberensis by Penicillium dioscin

“…Except for the traditional cultivation factors such as culture medium components and temperature, other new physical factors as selenium and lightening of special wavelengths [17] were reported to significantly enhance activity of fungus species as Cordyceps militaris. So new cultivation methods to promote hydrolysis activity of P. dioscin spawn deserves further research.…”

NMR and GC-MS Based Metabolic Profiling and Free-Radical Scavenging Activities of Cordyceps pruinosa Mycelia Cultivated under Different Media and Light Conditions

“…However, it is known that metabolite production is affected by environmental factors such as light exposure and culture medium components. Many previous studies have investigated the effects of environmental changes on the production of secondary metabolites in Cordyceps species [9]–[12]. In addition, there have been many studies of antioxidants from other Cordyceps species, including C. militaris and C. sinensis [13], [14].…”